دورية أكاديمية
Physicochemical and immunological effects of adjuvant formulations with snake venom antigens for immunization of horses for antivenom production.
| العنوان: | Physicochemical and immunological effects of adjuvant formulations with snake venom antigens for immunization of horses for antivenom production. |
|---|---|
| المؤلفون: | Fox CB; Access to Advanced Health Institute (formerly Infectious Disease Research Institute), Seattle, WA, USA; Department of Global Health, University of Washington, Seattle, WA, USA. Electronic address: christopher.fox@aahi.org., Khandhar AP; Access to Advanced Health Institute (formerly Infectious Disease Research Institute), Seattle, WA, USA., Khuu L; Access to Advanced Health Institute (formerly Infectious Disease Research Institute), Seattle, WA, USA., Phan T; Access to Advanced Health Institute (formerly Infectious Disease Research Institute), Seattle, WA, USA., Kinsey R; Access to Advanced Health Institute (formerly Infectious Disease Research Institute), Seattle, WA, USA., Cordero D; Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica., Gutiérrez JM; Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica., León G; Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica. |
| المصدر: | Toxicon : official journal of the International Society on Toxinology [Toxicon] 2023 Aug 15; Vol. 232, pp. 107229. Date of Electronic Publication: 2023 Jul 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 1307333 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3150 (Electronic) Linking ISSN: 00410101 NLM ISO Abbreviation: Toxicon Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford ; New York : Pergamon Press, c1962- |
| مواضيع طبية MeSH: | Antivenins*/pharmacology , Viperidae*, Horses ; Animals ; Emulsions ; Squalene ; Snake Venoms ; Adjuvants, Immunologic/pharmacology ; Immunization |
| مستخلص: | Enhancement of antivenom immune responses in horses through adjuvant technology improves antivenom production efficiency, but substantial local reactogenicity associated with some traditional veterinary adjuvants limits their usability. To explore modern adjuvant systems suitable for generating antivenom responses in horses, we first assessed their physicochemical compatibility with Bothrops asper snake venom. Liposome and nanoparticle aluminum adjuvants exhibited changes in particle size and phospholipid content after mixing with venom, whereas squalene emulsion-based adjuvants remained stable. Next, we evaluated serum antibody response magnitude and neutralization capacity in horses immunized with adjuvant-containing Echis ocellatus, Bitis arietans, Naja nigricollis, and Dendroaspis polylepis venom preparations. Whereas all tested adjuvants elicited significant neutralization capacity against the viperid venoms, the greatest antibody responses were generated by a squalene-in-water emulsion, thus representing a promising novel alternative for antivenom production. Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Authors declare no Competing Non-Financial Interests but the following Competing Financial Interests. CBF and APK are inventors on patents and/or patent applications involving adjuvant formulations and methods featured here. All other authors declare they have no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Adjuvant formulation; Adjuvant-antigen compatibility; Antivenom; Horse immunization; Snake venom; Snakebite |
| المشرفين على المادة: | 0 (Antivenins) 0 (Emulsions) 7QWM220FJH (Squalene) 0 (Snake Venoms) 0 (Adjuvants, Immunologic) |
| تواريخ الأحداث: | Date Created: 20230726 Date Completed: 20230808 Latest Revision: 20230808 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.toxicon.2023.107229 |
| PMID: | 37495191 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-3150 |
|---|---|
| DOI: | 10.1016/j.toxicon.2023.107229 |