دورية أكاديمية
أعرض في EDS

Prevalence of blood and skin trypanosomes in domestic and wild fauna from two sleeping sickness foci in Southern Cameroon.

التفاصيل البيبلوغرافية
العنوان: Prevalence of blood and skin trypanosomes in domestic and wild fauna from two sleeping sickness foci in Southern Cameroon.
المؤلفون: Magang EMK; Molecular Parasitology & Entomology Sub-unit, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.; Univ. Montpellier, CIRAD, IRD, Intertryp, Montpellier, France., Kamga RMN; Molecular Parasitology & Entomology Sub-unit, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon., Telleria J; Univ. Montpellier, CIRAD, IRD, Intertryp, Montpellier, France., Tichit M; Histopathology Platform, Institut Pasteur, Paris, France., Crouzols A; Trypanosome Transmission Group, Trypanosome Cell Biology Unit, INSERM U1201, Department of Parasites and Insect Vectors, Institut Pasteur Paris, Université Paris Cité, Paris, France., Kaboré J; Centre International de Recherche-Développement sur l'Elevage en zone Subhumide, Unité de recherche sur les maladies à vecteurs et biodiversité, Bobo-Dioulasso, Burkina Faso., Hardy D; Histopathology Platform, Institut Pasteur, Paris, France., Bouaka CUT; Centre for research in infectious disease, Yaoundé, Cameroon., Jamonneau V; Univ. Montpellier, CIRAD, IRD, Intertryp, Montpellier, France.; Unité de Recherche « Trypanosomoses », Institut Pierre Richet, Bouaké, Côte d'Ivoire., Rotureau B; Trypanosome Transmission Group, Trypanosome Cell Biology Unit, INSERM U1201, Department of Parasites and Insect Vectors, Institut Pasteur Paris, Université Paris Cité, Paris, France.; Parasitology Unit, Institut Pasteur of Guinea, Conakry, Guinea., Kuete V; Research Unit of Microbiology and Antimicrobial Substances, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon., Bart JM; Univ. Montpellier, CIRAD, IRD, Intertryp, Montpellier, France., Simo G; Molecular Parasitology & Entomology Sub-unit, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
المصدر: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2023 Jul 27; Vol. 17 (7), pp. e0011528. Date of Electronic Publication: 2023 Jul 27 (Print Publication: 2023).
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Trypanosomiasis, African*/epidemiology , Trypanosomiasis, African*/veterinary , Trypanosoma*/genetics , Tsetse Flies*/genetics, Humans ; Animals ; Swine ; Sheep ; Cameroon/epidemiology ; Prevalence ; DNA, Protozoan/genetics ; DNA, Protozoan/chemistry ; Trypanosoma brucei gambiense/genetics ; Animals, Wild ; Goats
مستخلص: Although studies on African Trypanosomiases revealed a variety of trypanosome species in the blood of various animal taxa, animal reservoirs of Trypanosoma brucei gambiense and anatomical niches such as skin have been overlooked in most epidemiological settings. This study aims to update epidemiological data on trypanosome infections in animals from human African trypanosomiasis (HAT) foci of Cameroon. Blood and skin snips were collected from 291 domestic and wild animals. DNA was extracted from blood and skin snips and molecular approaches were used to identify different trypanosomes species. Immunohistochemical analyses were used to confirm trypanosome infections in skin snips. PCR revealed 137 animals (47.1%) with at least one trypanosome species in the blood and/or in the skin. Of these 137 animals, 90 (65.7%) and 32 (23.4%) had trypanosome infections respectively in the blood and skin. Fifteen (10.9%) animals had trypanosome infections in both blood and skin snip. Animals from the Campo HAT focus (55.0%) were significantly (X2 = 17.6; P< 0.0001) more infected than those (29.7%) from Bipindi. Trypanosomes of the subgenus Trypanozoon were present in 27.8% of animals while T. vivax, T. congolense forest type and savannah type were detected in 16.5%, 10.3% and 1.4% of animals respectively. Trypanosoma b. gambiense infections were detected in the blood of 7.6% (22/291) of animals. No T. b. gambiense infection was detected in skin. This study highlights the presence of several trypanosome species in the blood and skin of various wild and domestic animals. Skin appeared as an anatomical reservoir for trypanosomes in animals. Despite methodological limitations, pigs, sheep, goats and wild animals were confirmed as potential reservoirs of T. b. gambiense. These animal reservoirs must be considered for the designing of control strategies that will lead to sustainable elimination of HAT.
Competing Interests: The authors have declared that they have no competing interests.
(Copyright: © 2023 Magang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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معلومات مُعتمدة: United Kingdom DH_ Department of Health; United Kingdom WT_ Wellcome Trust
المشرفين على المادة: 0 (DNA, Protozoan)
تواريخ الأحداث: Date Created: 20230727 Date Completed: 20230809 Latest Revision: 20230811
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10411957
DOI: 10.1371/journal.pntd.0011528
PMID: 37498955
قاعدة البيانات: MEDLINE
الوصف
تدمد:1935-2735
DOI:10.1371/journal.pntd.0011528