دورية أكاديمية

Collagen and Beyond: A Comprehensive Comparison of Human ECM Properties Derived from Various Tissue Sources for Regenerative Medicine Applications.

التفاصيل البيبلوغرافية
العنوان: Collagen and Beyond: A Comprehensive Comparison of Human ECM Properties Derived from Various Tissue Sources for Regenerative Medicine Applications.
المؤلفون: Patrawalla NY; Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology, Melbourne, FL 32901, USA., Kajave NS; Humabiologics® Inc., Phoenix, AZ 85034, USA., Albanna MZ; Humabiologics® Inc., Phoenix, AZ 85034, USA.; Department of General Surgery, Atrium Health Wake Forest Baptist, Winston-Salem, NC 27101, USA., Kishore V; Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology, Melbourne, FL 32901, USA.
المصدر: Journal of functional biomaterials [J Funct Biomater] 2023 Jul 11; Vol. 14 (7). Date of Electronic Publication: 2023 Jul 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101570734 Publication Model: Electronic Cited Medium: Print ISSN: 2079-4983 (Print) Linking ISSN: 20794983 NLM ISO Abbreviation: J Funct Biomater Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI AG
مستخلص: Collagen, along with proteoglycans, glycosaminoglycans, glycoproteins, and various growth factors, forms the extracellular matrix (ECM) and contributes to the complexity and diversity of different tissues. Herein, we compared the physicochemical and biological properties of ECM hydrogels derived from four different human tissues: skin, bone, fat, and birth. Pure human collagen type I hydrogels were used as control. Physical characterization of ECM hydrogels and assessment of cell response of cord-tissue mesenchymal stem cells (CMSCs) were performed. Decellularization efficiency was found to be >90% for all ECM. Hydroxyproline quantification assay showed that collagen content in birth ECM was comparable to collagen control and significantly greater than other sources of ECM. Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed the presence of γ, β, α 1 and α 2 collagen chains in all ECMs. Gelation kinetics of ECM hydrogels was significantly slower than collagen control. Compressive modulus of skin ECM was the highest and birth ECM was the lowest. Skin and birth ECM hydrogels were more stable than bone and fat ECM hydrogels. CMSCs encapsulated in birth ECM hydrogels exhibited the highest metabolic activity. Rheological characterization revealed that all ECM-derived inks exhibited shear thinning properties, and skin-derived ECM inks were most suitable for extrusion-based bioprinting for the concentration and printing conditions used in this study. Overall, results demonstrate that the physicochemical and biological properties of ECM hydrogels vary significantly depending on the tissue source. Therefore, careful selection of tissue source is important for development of ECM-based biomimetic tissue constructs for regenerative medicine applications.
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فهرسة مساهمة: Keywords: ECM; collagen; decellularization; human tissue; hydrogels; mesenchymal stem cells
تواريخ الأحداث: Date Created: 20230728 Latest Revision: 20230801
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10381652
DOI: 10.3390/jfb14070363
PMID: 37504858
قاعدة البيانات: MEDLINE