دورية أكاديمية
Staining Properties of Selected Commercial Fluorescent Dyes Toward B- and Z-DNA.
| العنوان: | Staining Properties of Selected Commercial Fluorescent Dyes Toward B- and Z-DNA. |
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| المؤلفون: | Bennett HA; Department of Chemistry and Centre for Biotechnology, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, Ontario, L2S 3A1, Canada., McAdorey A; Department of Chemistry and Centre for Biotechnology, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, Ontario, L2S 3A1, Canada., Yan H; Department of Chemistry and Centre for Biotechnology, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, Ontario, L2S 3A1, Canada. hyan@brocku.ca. |
| المصدر: | Journal of fluorescence [J Fluoresc] 2024 May; Vol. 34 (3), pp. 1193-1205. Date of Electronic Publication: 2023 Jul 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer- Country of Publication: Netherlands NLM ID: 9201341 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4994 (Electronic) Linking ISSN: 10530509 NLM ISO Abbreviation: J Fluoresc Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Springer- Original Publication: New York : Plenum Press, c1991- |
| مواضيع طبية MeSH: | Fluorescent Dyes*/chemistry , DNA, Z-Form*/chemistry , DNA, B-Form*/chemistry, Quinolines/chemistry ; Staining and Labeling ; Benzothiazoles/chemistry ; Diamines/chemistry ; Circular Dichroism ; Organic Chemicals/chemistry ; Spectrometry, Fluorescence ; DNA/chemistry |
| مستخلص: | The properties of six commonly used, commercially available, fluorescent dyes were compared in staining right-handed B-DNA and left-handed Z-DNA. All showed different degree of fluorescence turn-on in the presence of B-DNA, but very little in the presence of Z-DNA. The optimal range of dye-DNA ratios of DNA was determined. While these dyes do not provide a turn-on type probe for Z-DNA, staining between B- and Z-DNA using dyes such as SYBR Green I was shown to be useful in tracking the kinetics of conformational changes between these two forms of DNA. Finally, SYBR Green I showed unique circular dichroism patterns in 4 M NaCl that change in the presence of double stranded DNA, both in the visible and UV range. (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: DAPI; DNA staining dyes; Ethidium bromide; Eva Green; Propidium iodide; SYBR green I; SYBR safe |
| المشرفين على المادة: | 0 (Fluorescent Dyes) 0 (DNA, Z-Form) 0 (DNA, B-Form) 0 (Quinolines) 0 (Benzothiazoles) 163795-75-3 (SYBR Green I) 0 (Diamines) 0 (Organic Chemicals) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20230728 Date Completed: 20240501 Latest Revision: 20240501 |
| رمز التحديث: | 20240502 |
| DOI: | 10.1007/s10895-023-03343-8 |
| PMID: | 37505363 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1573-4994 |
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| DOI: | 10.1007/s10895-023-03343-8 |