دورية أكاديمية
Effects of BXSMD on ESR1 and ESR2 expression in CSD female mice.
| العنوان: | Effects of BXSMD on ESR1 and ESR2 expression in CSD female mice. |
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| المؤلفون: | Wang S; Shandong University of Traditional Chinese Medicine, Jinan, 250355, Shandong Province, PR China., Liu L; Shandong University of Traditional Chinese Medicine, Jinan, 250355, Shandong Province, PR China., Liang S; Shandong University of Traditional Chinese Medicine, Jinan, 250355, Shandong Province, PR China., Yang J; Shandong University of Traditional Chinese Medicine, Jinan, 250355, Shandong Province, PR China., Zhang Y; Shandong University of Traditional Chinese Medicine, Jinan, 250355, Shandong Province, PR China. Electronic address: zhangyan@sdutcm.edu.cn., Liu X; Shandong University of Traditional Chinese Medicine, Jinan, 250355, Shandong Province, PR China. Electronic address: 60020069@sdutcm.edu.cn. |
| المصدر: | Journal of ethnopharmacology [J Ethnopharmacol] 2024 Jan 10; Vol. 318 (Pt B), pp. 116973. Date of Electronic Publication: 2023 Jul 28. |
| نوع المنشور: | Journal Article; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Sequoia Original Publication: Lausanne, Elsevier Sequoia. |
| مواضيع طبية MeSH: | Estrogen Receptor beta*/genetics , Estrogen Receptor beta*/metabolism , Sleep Initiation and Maintenance Disorders*, Mice ; Female ; Animals ; Estrogen Receptor alpha/genetics ; Sleep Deprivation ; Serotonin ; Tandem Mass Spectrometry ; RNA, Messenger/metabolism ; gamma-Aminobutyric Acid |
| مستخلص: | Ethnic Pharmacological Relevance: Due to the rapid pace of modern society, chronic insomnia has become universal phenomenon. In China, Banxia Shumi Decoction (BXSMD) has been used in treating chronic insomnia for thousands of years, but its chemical composition and action mechanism are still unknown. Aim of the Study: This study aims to explore the chemical composition of BXSMD and its effects on Estrogen receptor 1 (ESR1) and Estrogen receptor 2 (ESR2) in mice with chronic sleep deprivation (CSD). Materials and Methods: UHPLC-Q-Orbitrap-MS/MS was applied in determining the chemical composition of BXSMD. After 21-day sleep deprivation (SD) in platform water environment, CSD mice model was prepared. By conducting open field test, 24-h autonomic diurnal and nocturnal activity of mice in each group was detected. ELISA was employed to measure the contents of 5-HT, DA, NE, GABA, Glu, and MT. With RT-PCR, Western blot (WB), and immunohistochemistry (IHC), mRNA and protein expressions of ESR1 and ESR2 in the hypothalamus and hippocampus were tested. Results: BXSMD included ferulic acid, kaverol, daidzein, apigenin, berberine, adenosine, aesculin, vanillin, naringin, and glycine, which might constitute the material basis forthe improvement of chronic insomnia. With BXSMD, the total moving distance and the rest time in dark period of CSD mice were shortened, while its rest time in light period was increased. Besides, BXSMD enhanced the contents of 5-HT, GABA, and MT in CSD mice, and decreased the contents of Glu, NE, and DA. BXSMD elevated the mRNA of Esr1 and Esr2, and elevated the protein expressions of ESR1 and ESR2 in the hypothalamus and hippocampus of CSD mice. Conclusions: BXSMD contains various chemical components for sleep-wake regulation, with the mechanism of stimulating estrogen signaling pathway by regulating the expressions of ESR1 and ESR2, ultimately realizing the regulation to sleep-wake disorder caused by CSD. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Banxia shumi decoction; Chronic sleep deprivation; Circadian rhythm; ESR1; ESR2; Sleep homeostasis |
| المشرفين على المادة: | 0 (Estrogen Receptor beta) 0 (Estrogen Receptor alpha) 333DO1RDJY (Serotonin) 0 (RNA, Messenger) 56-12-2 (gamma-Aminobutyric Acid) |
| تواريخ الأحداث: | Date Created: 20230730 Date Completed: 20230918 Latest Revision: 20230918 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.jep.2023.116973 |
| PMID: | 37517566 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7573 |
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| DOI: | 10.1016/j.jep.2023.116973 |