دورية أكاديمية
Knockout of all ErbB-family genes delineates their roles in proliferation, survival and migration.
| العنوان: | Knockout of all ErbB-family genes delineates their roles in proliferation, survival and migration. |
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| المؤلفون: | Matsuda K; Research Center for Dynamic Living Systems, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan., Hirayama D; Research Center for Dynamic Living Systems, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan., Hino N; Research Center for Dynamic Living Systems, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan., Kuno S; Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan., Sakaue-Sawano A; Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan., Miyawaki A; Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan., Matsuda M; Research Center for Dynamic Living Systems, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.; Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.; Institute for Integrated Cell-Material Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan., Terai K; Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan. |
| المصدر: | Journal of cell science [J Cell Sci] 2023 Aug 15; Vol. 136 (16). Date of Electronic Publication: 2023 Aug 21. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company of Biologists Country of Publication: England NLM ID: 0052457 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9137 (Electronic) Linking ISSN: 00219533 NLM ISO Abbreviation: J Cell Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge : Company of Biologists Original Publication: London. |
| مواضيع طبية MeSH: | Signal Transduction* , Receptor, ErbB-2*/genetics , Receptor, ErbB-2*/metabolism, Animals ; Dogs ; Ligands ; Phosphorylation ; Genes, erbB ; Cell Proliferation/genetics ; Receptor, ErbB-3/genetics ; Receptor, ErbB-3/metabolism |
| مستخلص: | The ErbB-family receptors play pivotal roles in the proliferation, migration and survival of epithelial cells. Because our knowledge on the ErbB-family receptors has been largely obtained by the exogenous application of their ligands, it remains unknown to what extent each of the ErbB members contributes to these outputs. We here knocked out each ErbB gene, various combinations of ErbB genes or all ErbB genes in Madin-Darby canine kidney cells to delineate the contribution of each gene. ERK1 and ERK2 (ERK1/2, also known as MAPK3 and MAPK1, respectively) activation waves during collective cell migration were mediated primarily by ErbB1 and secondarily by the ErbB2 and ErbB3 heterodimer. Either ErbB1 or the ErbB2 and ErbB3 complex was sufficient for the G1/S progression. The saturation cell density was markedly reduced in cells deficient in all ErbB proteins, but not in cells retaining only ErbB2, which cannot bind to ligands. Thus, a ligand-independent ErbB2 activity is sufficient for preventing apoptosis at high cell density. In short, systematic knockout of ErbB-family genes has delineated the roles of each ErbB receptor. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2023. Published by The Company of Biologists Ltd.) |
| فهرسة مساهمة: | Keywords: Cell proliferation; Contact inhibition; EGF; ErbB; Saturation cell density |
| المشرفين على المادة: | 0 (Ligands) EC 2.7.10.1 (Receptor, ErbB-2) EC 2.7.10.1 (Receptor, ErbB-3) |
| تواريخ الأحداث: | Date Created: 20230731 Date Completed: 20230822 Latest Revision: 20230822 |
| رمز التحديث: | 20230822 |
| DOI: | 10.1242/jcs.261199 |
| PMID: | 37519219 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9137 |
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| DOI: | 10.1242/jcs.261199 |