دورية أكاديمية
Selective expression of light-harvesting complexes alters phospholipid composition in the intracytoplasmic membrane and core complex of purple phototrophic bacteria.
| العنوان: | Selective expression of light-harvesting complexes alters phospholipid composition in the intracytoplasmic membrane and core complex of purple phototrophic bacteria. |
|---|---|
| المؤلفون: | Satoh I; Faculty of Science, Ibaraki University, Mito 310-8512, Japan., Gotou K; Faculty of Science, Ibaraki University, Mito 310-8512, Japan., Nagatsuma S; Faculty of Science, Ibaraki University, Mito 310-8512, Japan., Nagashima KVP; Research Institute for Integrated Science, Kanagawa University, 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama, Kanagawa 221-8686, Japan., Kobayashi M; National Institute of Technology, Ariake College, Omuta, Fukuoka 836-8585, Japan., Yu LJ; Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China., Madigan MT; School of Biological Sciences, Department of Microbiology, Southern Illinois University, Carbondale, IL 62901, USA., Kimura Y; Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Nada, Kobe 657-8501, Japan., Wang-Otomo ZY; Faculty of Science, Ibaraki University, Mito 310-8512, Japan. Electronic address: wang@ml.ibaraki.ac.jp. |
| المصدر: | Biochimica et biophysica acta. Bioenergetics [Biochim Biophys Acta Bioenerg] 2023 Nov 01; Vol. 1864 (4), pp. 149001. Date of Electronic Publication: 2023 Jul 30. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101731706 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-2650 (Electronic) Linking ISSN: 00052728 NLM ISO Abbreviation: Biochim Biophys Acta Bioenerg Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier |
| مواضيع طبية MeSH: | Proteobacteria*/metabolism , Rhodobacter sphaeroides*/genetics , Rhodobacter sphaeroides*/metabolism, Phospholipids/metabolism ; Light-Harvesting Protein Complexes/genetics ; Light-Harvesting Protein Complexes/metabolism ; Cardiolipins/metabolism ; Carotenoids/metabolism |
| مستخلص: | Phospholipid-protein interactions play important roles in regulating the function and morphology of photosynthetic membranes in purple phototrophic bacteria. Here, we characterize the phospholipid composition of intracytoplasmic membrane (ICM) from Rhodobacter (Rba.) sphaeroides that has been genetically altered to selectively express light-harvesting (LH) complexes. In the mutant strain (DP2) that lacks a peripheral light-harvesting (LH2) complex, the phospholipid composition was significantly different from that of the wild-type strain; strain DP2 showed a marked decrease in phosphatidylglycerol (PG) and large increases in cardiolipin (CL) and phosphatidylcholine (PC) indicating preferential interactions between the complexes and specific phospholipids. Substitution of the core light-harvesting (LH1) complex of Rba. sphaeroides strain DP2 with that from the purple sulfur bacterium Thermochromatium tepidum further altered the phospholipid composition, with substantial increases in PG and PE and decreases in CL and PC, indicating that the phospholipids incorporated into the ICM depend on the nature of the LH1 complex expressed. Purified LH1-reaction center core complexes (LH1-RC) from the selectively expressing strains also contained different phospholipid compositions than did core complexes from their corresponding wild-type strains, suggesting different patterns of phospholipid association between the selectively expressed LH1-RC complexes and those purified from native strains. Effects of carotenoids on the phospholipid composition were also investigated using carotenoid-suppressed cells and carotenoid-deficient species. The findings are discussed in relation to ICM morphology and specific LH complex-phospholipid interactions. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Carotenoid; Light-harvesting; Membrane morphology; Reaction center; Rhodobacter sphaeroides |
| المشرفين على المادة: | 0 (Phospholipids) 0 (Light-Harvesting Protein Complexes) 0 (Cardiolipins) 36-88-4 (Carotenoids) |
| تواريخ الأحداث: | Date Created: 20230801 Date Completed: 20230911 Latest Revision: 20230925 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.bbabio.2023.149001 |
| PMID: | 37527691 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-2650 |
|---|---|
| DOI: | 10.1016/j.bbabio.2023.149001 |