دورية أكاديمية
Maternal Toxoplasma gondii infection affects proliferation, differentiation and cell cycle regulation of retinal neural progenitor cells in mouse embryo.
| العنوان: | Maternal Toxoplasma gondii infection affects proliferation, differentiation and cell cycle regulation of retinal neural progenitor cells in mouse embryo. |
|---|---|
| المؤلفون: | de Campos VS; Laboratório de Neurobiologia da Retina, Instituto de Biologia, Universidade Federal Fluminense, Niterói, Brazil.; Laboratório de Biologia Estrutural, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil., Magalhães CF; Laboratório de Neurobiologia da Retina, Instituto de Biologia, Universidade Federal Fluminense, Niterói, Brazil., da Rosa BG; Laboratório de Biologia Estrutural, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil., Dos Santos CM; Laboratório de Biologia Estrutural, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil., Fragel-Madeira L; Laboratório de Desenvolvimento e Regeneração Neural, Instituto de Biologia, Universidade Federal Fluminense, Niterói, Brazil., Figueiredo DP; Laboratório de Neurobiologia da Retina, Instituto de Biologia, Universidade Federal Fluminense, Niterói, Brazil., Calaza KC; Laboratório de Neurobiologia da Retina, Instituto de Biologia, Universidade Federal Fluminense, Niterói, Brazil., Adesse D; Laboratório de Biologia Estrutural, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil.; Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL, United States. |
| المصدر: | Frontiers in cellular neuroscience [Front Cell Neurosci] 2023 Jul 21; Vol. 17, pp. 1211446. Date of Electronic Publication: 2023 Jul 21 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101477935 Publication Model: eCollection Cited Medium: Print ISSN: 1662-5102 (Print) Linking ISSN: 16625102 NLM ISO Abbreviation: Front Cell Neurosci Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne, Switzerland : Frontiers Research Foundation, 2007- |
| مستخلص: | Background: Toxoplasmosis affects one third of the world population and has the protozoan Toxoplasma gondii as etiological agent. Congenital toxoplasmosis (CT) can cause severe damage to the fetus, including miscarriages, intracranial calcification, hydrocephalus and retinochoroiditis. Severity of CT depends on the gestational period in which infection occurs, and alterations at the cellular level during retinal development have been reported. In this study, we proposed a mouse CT model to investigate the impact of infection on retinal development. Methods: Pregnant females of pigmented C57BL/6 strain mice were infected intragastrically with two T. gondii cysts (ME49 strain) at embryonic day 10 (E10), and the offspring were analyzed at E18. Results: Infected embryos had significantly smaller body sizes and weights than the PBS-treated controls, indicating that embryonic development was affected. In the retina, a significant increase in the number of Ki-67-positive cells (marker of proliferating cells) was found in the apical region of the NBL of infected mice compared to the control. Supporting this, cell cycle proteins Cyclin D3, Cdk6 and pChK2 were significantly altered in infected retinas. Interestingly, the immunohistochemical analysis showed a significant increase in the population of β-III-tubulin-positive cells, one of the earliest markers of neuronal differentiation. Conclusions: Our data suggests that CT affects cell cycle progression in retinal progenitor cells, possibly inducing the arrest of these cells at G2/M phase. Such alterations could influence the differentiation, anticipating/increasing neuronal maturation, and therefore leading to abnormal retinal formation. Our model mimics important events observed in ocular CT. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Campos, Magalhães, da Rosa, dos Santos, Fragel-Madeira, Figueiredo, Calaza and Adesse.) |
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| فهرسة مساهمة: | Keywords: TORCH infections; Toxoplasma gondii; cell cycle proteins; congenital toxoplasmosis; cyclins; retinal neurogenesis; retinal progenitor cells |
| تواريخ الأحداث: | Date Created: 20230807 Latest Revision: 20231102 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10400775 |
| DOI: | 10.3389/fncel.2023.1211446 |
| PMID: | 37545879 |
| قاعدة البيانات: | MEDLINE |
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