دورية أكاديمية
أعرض في EDS

Near chromosome-level genome assembly of the microsporidium Hamiltosporidium tvaerminnensis.

التفاصيل البيبلوغرافية
العنوان: Near chromosome-level genome assembly of the microsporidium Hamiltosporidium tvaerminnensis.
المؤلفون: Angst P; Department of Environmental Sciences, Zoology, University of Basel, Basel 4051, Switzerland., Pombert JF; Department of Biology, Illinois Institute of Technology, Chicago, IL 60616, USA., Ebert D; Department of Environmental Sciences, Zoology, University of Basel, Basel 4051, Switzerland., Fields PD; Department of Environmental Sciences, Zoology, University of Basel, Basel 4051, Switzerland.
المصدر: G3 (Bethesda, Md.) [G3 (Bethesda)] 2023 Sep 30; Vol. 13 (10).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 101566598 Publication Model: Print Cited Medium: Internet ISSN: 2160-1836 (Electronic) Linking ISSN: 21601836 NLM ISO Abbreviation: G3 (Bethesda) Subsets: MEDLINE
أسماء مطبوعة: Publication: 2021- : [Oxford] : Oxford University Press
Original Publication: Bethesda, MD : Genetics Society of America, 2011-
مواضيع طبية MeSH: Microsporidia, Unclassified*/genetics , Microsporidia*/genetics, Chromosomes ; Genomics ; Molecular Sequence Annotation
مستخلص: Microsporidia are intracellular parasitic fungi whose genomes rank among the smallest of all known eukaryotes. A number of outstanding questions remain concerning the evolution of their large-scale variation in genome architecture, responsible for genome size variation of more than an order of magnitude. This genome report presents the first near-chromosomal assembly of a large-genome microsporidium, Hamiltosporidium tvaerminnensis. Combined Oxford Nanopore, Pacific Biosciences (PacBio), and Illumina sequencing led to a genome assembly of 17 contigs, 11 of which represent complete chromosomes. Our assembly is 21.64 Mb in length, has an N50 of 1.44 Mb, and consists of 39.56% interspersed repeats. We introduce a novel approach in microsporidia, PacBio Iso-Seq, as part of a larger annotation pipeline for obtaining high-quality annotations of 3,573 protein-coding genes. Based on direct evidence from the full-length Iso-Seq transcripts, we present evidence for alternative polyadenylation and variation in splicing efficiency, which are potential regulation mechanisms for gene expression in microsporidia. The generated high-quality genome assembly is a necessary resource for comparative genomics that will help elucidate the evolution of genome architecture in response to intracellular parasitism.
Competing Interests: Conflicts of interest The authors declare no conflict of interest.
(© The Author(s) 2023. Published by Oxford University Press on behalf of The Genetics Society of America.)
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فهرسة مساهمة: Keywords: Hamiltosporidium tvaerminnensis; Iso-Seq; alternative polyadenylation; fungi; microsporidia; splicing efficiency; telomere-to-telomere
سلسلة جزيئية: figshare 10.6084/m9.figshare.22794887
SCR Organism: Hamiltosporidium tvaerminnensis
تواريخ الأحداث: Date Created: 20230811 Date Completed: 20231002 Latest Revision: 20231004
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10542269
DOI: 10.1093/g3journal/jkad185
PMID: 37565496
قاعدة البيانات: MEDLINE
الوصف
تدمد:2160-1836
DOI:10.1093/g3journal/jkad185