دورية أكاديمية
Development of a Standardized Soybean Cyst Nematode Screening Assay in Pennycress and Identification of Resistant Germplasm.
| العنوان: | Development of a Standardized Soybean Cyst Nematode Screening Assay in Pennycress and Identification of Resistant Germplasm. |
|---|---|
| المؤلفون: | Basnet P; Department of Plant and Agroecosystem Sciences, University of Wisconsin, Madison, WI.; Division of Plant Science and Technology, University of Missouri, Columbia, MO., Meinhardt CG; Division of Plant Science and Technology, University of Missouri, Columbia, MO., Dhital B; Division of Plant Science and Technology, University of Missouri, Columbia, MO., Nguyen A; Division of Plant Science and Technology, University of Missouri, Columbia, MO., Gillman JD; Plant Genetics Research Unit, USDA-ARS, Columbia, MO., Joshi T; Department of Health Management and Informatics, MUIDSI, and Bond Life Sciences Center, University of Missouri, Columbia, MO., Mitchum MG; Department of Plant Pathology and Institute of Plant Breeding, Genetics, and Genomics, University of Georgia, Athens, GA., Scaboo AM; Division of Plant Science and Technology, University of Missouri, Columbia, MO. |
| المصدر: | Plant disease [Plant Dis] 2024 Feb; Vol. 108 (2), pp. 359-364. Date of Electronic Publication: 2024 Feb 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Phytopathological Society Country of Publication: United States NLM ID: 9882809 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0191-2917 (Print) Linking ISSN: 01912917 NLM ISO Abbreviation: Plant Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: St. Paul, Minn. : American Phytopathological Society, [1980]- |
| مواضيع طبية MeSH: | Nematoda* , Cysts*, Animals ; Glycine max ; Soil ; Seeds |
| مستخلص: | The prospect of incorporating pennycress as an oilseed cover crop in the Midwest's corn-soybean rotation system has drawn researcher and farmer attention. The inclusion of pennycress will be beneficial as it provides an excellent soil cover to reduce soil erosion and nutrient leaching while serving as an additional source for oilseed production and income. However, pennycress is an alternative host for soybean cyst nematode (SCN), which is a major biological threat to soybean that needs to be addressed for sustainable pennycress adoption into our current production systems. To develop a standardized SCN resistance screening strategy in pennycress, we tested and optimized five parameters: (i) germination stimulants, (ii) inoculation timing, (iii) inoculation rate, (iv) experimental incubation time, and (v) susceptible checks. The standardized SCN resistance screening protocol includes the following: (i) treating pennycress seeds with gibberellic acid for 24 h, (ii) transplanting seedlings 12 to 15 days after initiating germination and inoculating 10 to 12 days after transplantation, (iii) inoculating at a rate of 1,500 eggs/100 cc soil (1,500 eggs per plant), (iv) processing roots at 30 days after inoculation, and (v) using susceptible pennycress accession Ames 32869 to calculate the female index. The standardized protocol was used to quantify the response of a diverse set of pennycress accessions for response against SCN HG type 1.2.5.7 and HG type 7. While there were no highly resistant pennycress lines identified, 15 were rated as moderately resistant to HG type 1.2.5.7, and eight were rated moderately resistant to HG type 7. The resistant lines identified in this study could be utilized to develop SCN-resistant pennycress cultivars. The study also opens a new avenue for research to understand SCN-pennycress interactions through molecular and genomic studies. This knowledge could aid in the successful inclusion of pennycress as a beneficial cover/oilseed crop in the United States Midwest.[Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. Competing Interests: The author(s) declare no conflict of interest. |
| فهرسة مساهمة: | Keywords: cover crop; disease management; pennycress; resistant screening; soybean cyst nematode |
| المشرفين على المادة: | 0 (Soil) |
| تواريخ الأحداث: | Date Created: 20230814 Date Completed: 20240301 Latest Revision: 20240301 |
| رمز التحديث: | 20240301 |
| DOI: | 10.1094/PDIS-05-23-0858-RE |
| PMID: | 37578367 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0191-2917 |
|---|---|
| DOI: | 10.1094/PDIS-05-23-0858-RE |