دورية أكاديمية
أعرض في EDS

Src family kinases engage differential pathways for encapsulation into extracellular vesicles.

التفاصيل البيبلوغرافية
العنوان: Src family kinases engage differential pathways for encapsulation into extracellular vesicles.
المؤلفون: Ye C; Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia Athens, Athens, Georgia 30602., Gosser C; Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia Athens, Athens, Georgia 30602., Runyon ED; Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia Athens, Athens, Georgia 30602., Zha J; Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia Athens, Athens, Georgia 30602., Cai J; Department of Cellular Biology and Anatomy, Augusta University, Augusta, Georgia 30912., Beharry Z; Department of Chemical and Physical Sciences, University of Virgin Islands, St. Thomas, VI 00802., Rickman CB; Department of Ophthalmology, Duke University, Durham, NC 27710.; Department of Cell Biology, Duke University, Durham, NC 27710., Klingeborn M; Department of Ophthalmology, Duke University, Durham, NC 27710.; Current address: McLaughlin Research Institute, Great Falls, MT 59405., Liu Y; Department of Cellular Biology and Anatomy, Augusta University, Augusta, Georgia 30912., Xie J; Department of Chemistry, University of Georgia Athens, Athens, Georgia 30602., Cai H; Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia Athens, Athens, Georgia 30602.
المصدر: Journal of extracellular biology [J Extracell Biol] 2023 Jun; Vol. 2 (6). Date of Electronic Publication: 2023 Jun 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles Country of Publication: United States NLM ID: 9918382980506676 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2768-2811 (Electronic) Linking ISSN: 27682811 NLM ISO Abbreviation: J Extracell Biol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Hoboken, New Jersey] : Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles, [2022]-
مستخلص: Extracellular vesicles (EVs) are heterogeneous biological nanoparticles secreted by all cell types. Identifying the proteins preferentially encapsulated in secreted EVs will help understand their heterogeneity. Src family kinases including Src and Fyn are a group of tyrosine kinases with fatty acylation modifications and/or multiple lysine residues (contributing charge interaction) at their N-terminus. Here, we demonstrate that Src and Fyn kinases were preferentially encapsulated in EVs and fatty acylation including myristoylation and palmitoylation facilitated their encapsulation. Genetic loss or pharmacological inhibition of myristoylation suppressed Src and/or Fyn kinase levels in EVs. Similarly, loss of palmitoylation reduced Fyn levels in EVs. Additionally, mutation of lysine at sites 5, 7, and 9 of Src kinase also inhibited the encapsulation of myristoylated Src into EVs. Knockdown of TSG101, which is a protein involved in the endosomal sorting complexes required for transport (ESCRT) protein complex mediated EVs biogenesis and led to a reduction of Src levels in EVs. In contrast, filipin III treatment, which disturbed the lipid raft structure, reduced Fyn kinase levels, but not Src kinase levels in EVs. Finally, elevated levels of Src protein were detected in the serum EVs of host mice carrying constitutively active Src-mediated prostate tumors in vivo . Collectively, the data suggest that different EVs biogenesis pathways exist and can regulate the encapsulation of specific proteins into EVs. This study provides an understanding of the EVs heterogeneity created by different EVs biogenesis pathways.
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معلومات مُعتمدة: R21 AI157831 United States AI NIAID NIH HHS; R21 AI171944 United States AI NIAID NIH HHS; R01 CA257851 United States CA NCI NIH HHS; U01 CA225784 United States CA NCI NIH HHS; R21 EY033057 United States EY NEI NIH HHS; R01 CA247769 United States CA NCI NIH HHS; P30 EY031631 United States EY NEI NIH HHS; R21 EY028671 United States EY NEI NIH HHS; P30 EY005722 United States EY NEI NIH HHS; R01 EY031748 United States EY NEI NIH HHS; R01 CA172495 United States CA NCI NIH HHS
فهرسة مساهمة: Keywords: Myristoylation; Src family kinases; extracellular vesicles; heterogeneity; palmitoylation
تواريخ الأحداث: Date Created: 20230817 Latest Revision: 20240613
رمز التحديث: 20240613
مُعرف محوري في PubMed: PMC10426749
DOI: 10.1002/jex2.96
PMID: 37588411
قاعدة البيانات: MEDLINE
الوصف
تدمد:2768-2811
DOI:10.1002/jex2.96