دورية أكاديمية
أعرض في EDS

Z-Form Extracellular DNA in Pediatric CRS May Provide a Mechanism for Recalcitrance to Treatment.

التفاصيل البيبلوغرافية
العنوان: Z-Form Extracellular DNA in Pediatric CRS May Provide a Mechanism for Recalcitrance to Treatment.
المؤلفون: Hofer LK; Center for Microbial Pathogenesis, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, Ohio, U.S.A., Jurcisek JA; Center for Microbial Pathogenesis, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, Ohio, U.S.A., Elmaraghy C; The Ohio State University College of Medicine, Columbus, Ohio, U.S.A.; Department of Otolaryngology-Head and Neck Surgery, The Ohio State University Wexner Medical Center, Columbus, Ohio, U.S.A.; Department of Pediatric Otolaryngology, Nationwide Children's Hospital, Columbus, Ohio, U.S.A., Goodman SD; Center for Microbial Pathogenesis, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, Ohio, U.S.A.; The Ohio State University College of Medicine, Columbus, Ohio, U.S.A., Bakaletz LO; Center for Microbial Pathogenesis, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, Ohio, U.S.A.; The Ohio State University College of Medicine, Columbus, Ohio, U.S.A.
المصدر: The Laryngoscope [Laryngoscope] 2024 Apr; Vol. 134 (4), pp. 1564-1571. Date of Electronic Publication: 2023 Aug 19.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 8607378 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1531-4995 (Electronic) Linking ISSN: 0023852X NLM ISO Abbreviation: Laryngoscope Subsets: MEDLINE
أسماء مطبوعة: Publication: <2009- >: Philadelphia, PA : Wiley-Blackwell
Original Publication: St. Louis, Mo. : [s.n., 1896-
مواضيع طبية MeSH: DNA, Z-Form* , DNA, B-Form* , Sinusitis*/surgery , Rhinitis*/surgery, Humans ; Child ; Integration Host Factors ; Biofilms ; Chronic Disease
مستخلص: Objectives: We examined sinus mucosal samples recovered from pediatric chronic rhinosinusitis (CRS) patients for the presence of Z-form extracellular DNA (eDNA) due to its recently elucidated role in pathogenesis of disease. Further, we immunolabeled these specimens for the presence of both members of the bacterial DNA-binding DNABII protein family, integration host factor (IHF) and histone-like protein (HU), due to their known role in converting common B-DNA to the rare Z-form.
Methods: Sinus mucosa samples recovered from 20 patients during functional endoscopic sinus surgery (FESS) were immunolabelled for B- and Z-DNA, as well as for both bacterial DNABII proteins.
Results: Nineteen of 20 samples (95%) included areas rich in eDNA, with the majority in the Z-form. Areas positive for B-DNA were restricted to the most distal regions of the mucosal specimen. Labeling for both DNABII proteins was observed on B- and Z-DNA, which aligned with the role of these proteins in the B-to-Z DNA conversion.
Conclusions: Abundant Z-form eDNA in culture-positive pediatric CRS samples suggested that bacterial DNABII proteins were responsible for the conversion of eukaryotic B-DNA that had been released into the luminal space by PMNs during NETosis, to the Z-form. The presence of both DNABII proteins on B-DNA and Z-DNA supported the known role of these bacterial proteins in the B-to-Z DNA conversion. Given that Z-form DNA both stabilizes the bacterial biofilm and inactivates PMN NET-mediated killing of trapped bacteria, we hypothesize that this conversion may be contributing to the chronicity and recalcitrance of CRS to treatment.
Level of Evidence: NA Laryngoscope, 134:1564-1571, 2024.
(© 2023 The American Laryngological, Rhinological and Otological Society, Inc.)
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معلومات مُعتمدة: R01 DC003915 United States DC NIDCD NIH HHS; R01 DC011818 United States DC NIDCD NIH HHS
فهرسة مساهمة: Keywords: Biofilm; DNABII Proteins; FESS; PMNs
المشرفين على المادة: 0 (DNA, Z-Form)
0 (DNA, B-Form)
0 (Integration Host Factors)
تواريخ الأحداث: Date Created: 20230819 Date Completed: 20240318 Latest Revision: 20240416
رمز التحديث: 20240416
مُعرف محوري في PubMed: PMC10875147
DOI: 10.1002/lary.30986
PMID: 37597166
قاعدة البيانات: MEDLINE
الوصف
تدمد:1531-4995
DOI:10.1002/lary.30986