التفاصيل البيبلوغرافية
| العنوان: |
Structural basis of archaeal FttA-dependent transcription termination. |
| المؤلفون: |
Wang C, Molodtsov V, Sanders TJ, Marshall CJ, Firlar E, Kaelber JT, Santangelo TJ, Ebright RH |
| المصدر: |
BioRxiv : the preprint server for biology [bioRxiv] 2023 Aug 10. Date of Electronic Publication: 2023 Aug 10. |
| نوع المنشور: |
Preprint |
| اللغة: |
English |
| بيانات الدورية: |
Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: |
The ribonuclease FttA mediates factor-dependent transcription termination in archaea 1-3 . Here, we report the structure of a Thermococcus kodakarensis transcription pre-termination complex comprising FttA, Spt4, Spt5, and a transcription elongation complex (TEC). The structure shows that FttA interacts with the TEC in a manner that enables RNA to proceed directly from the TEC RNA-exit channel to the FttA catalytic center and that enables endonucleolytic cleavage of RNA by FttA, followed by 5'→3' exonucleolytic cleavage of RNA by FttA and concomitant 5'→3' translocation of FttA on RNA, to apply mechanical force to the TEC and trigger termination. The structure further reveals that Spt5 bridges FttA and the TEC, explaining how Spt5 stimulates FttA-dependent termination. The results reveal functional analogy between bacterial and archaeal factor-dependent termination, reveal functional homology between archaeal and eukaryotic factor-dependent termination, and reveal fundamental mechanistic similarities in factor-dependent termination in the three domains of life: bacterial, archaeal, and eukaryotic.
One Sentence Summary: Cryo-EM reveals the structure of the archaeal FttA pre-termination complex. |
| معلومات مُعتمدة: |
R01 GM100329 United States GM NIGMS NIH HHS; R35 GM143963 United States GM NIGMS NIH HHS |
| تواريخ الأحداث: |
Date Created: 20230823 Latest Revision: 20240214 |
| رمز التحديث: |
20240214 |
| مُعرف محوري في PubMed: |
PMC10441395 |
| DOI: |
10.1101/2023.08.09.552649 |
| PMID: |
37609354 |
| قاعدة البيانات: |
MEDLINE |
