دورية أكاديمية
Expansion of bovine skeletal muscle stem cells from spinner flasks to benchtop stirred-tank bioreactors for up to 38 days.
| العنوان: | Expansion of bovine skeletal muscle stem cells from spinner flasks to benchtop stirred-tank bioreactors for up to 38 days. |
|---|---|
| المؤلفون: | Tzimorotas D; Nofima AS, Food Safety and Health, Ås, Norway., Solberg NT; Nofima AS, Raw Materials and Process Optimization, Ås, Norway., Andreassen RC; Nofima AS, Raw Materials and Process Optimization, Ås, Norway., Moutsatsou P; Mosa Meat BV, Maastricht, Netherlands., Bodiou V; Mosa Meat BV, Maastricht, Netherlands., Pedersen ME; Nofima AS, Raw Materials and Process Optimization, Ås, Norway., Rønning SB; Nofima AS, Raw Materials and Process Optimization, Ås, Norway. |
| المصدر: | Frontiers in nutrition [Front Nutr] 2023 Aug 07; Vol. 10, pp. 1192365. Date of Electronic Publication: 2023 Aug 07 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media S. A Country of Publication: Switzerland NLM ID: 101642264 Publication Model: eCollection Cited Medium: Print ISSN: 2296-861X (Print) Linking ISSN: 2296861X NLM ISO Abbreviation: Front Nutr Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne, Switzerland : Frontiers Media S. A., [2014]- |
| مستخلص: | Introduction: Successful long-term expansion of skeletal muscle satellite cells (MuSCs) on a large scale is fundamental for cultivating animal cells for protein production. Prerequisites for efficient cell expansion include maintaining essential native cell activities such as cell adhesion, migration, proliferation, and differentiation while ensuring consistent reproducibility. Method: This study investigated the growth of bovine MuSC culture using low-volume spinner flasks and a benchtop stirred-tank bioreactor (STR). Results and Discussion: Our results showed for the first time the expansion of primary MuSCs for 38 days in a bench-top STR run with low initial seeding density and FBS reduction, supported by increased expression of the satellite cell marker PAX7 and reduced expression of differentiation-inducing genes like MYOG, even without adding p38-MAPK inhibitors. Moreover, the cells retained their ability to proliferate, migrate, and differentiate after enzymatic dissociation from the microcarriers. We also showed reproducible results in a separate biological benchtop STR run. Competing Interests: PM and VB were employed by Mosa Meat, BV. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Tzimorotas, Solberg, Andreassen, Moutsatsou, Bodiou, Pedersen and Rønning.) |
| References: | ALTEX. 2011;28(4):305-16. (PMID: 22130485) Differentiation. 2013 Jul-Sep;86(1-2):13-22. (PMID: 23933398) APMIS. 2001 Nov;109(11):726-34. (PMID: 11900051) Commun Biol. 2022 Jun 2;5(1):466. (PMID: 35654948) J Sci Food Agric. 2014 Apr;94(6):1039-41. (PMID: 24214798) Front Bioeng Biotechnol. 2022 Aug 04;10:895289. (PMID: 35992337) Nat Rev Mol Cell Biol. 2016 May;17(5):280-92. (PMID: 27033256) J Cell Mol Med. 2004 Oct-Dec;8(4):413-22. (PMID: 15601570) Stem Cell Rev Rep. 2012 Jun;8(2):609-22. (PMID: 22278133) Sci Rep. 2023 Jan 10;13(1):498. (PMID: 36627406) J Histochem Cytochem. 2010 Nov;58(11):941-55. (PMID: 20644208) Science. 1995 Feb 17;267(5200):1022-4. (PMID: 7863328) Biotechnol Bioeng. 2020 Oct;117(10):3029-3039. (PMID: 32568406) Stem Cells Transl Med. 2021 Sep;10(9):1329-1342. (PMID: 34008349) Semin Cell Dev Biol. 2017 Dec;72:19-32. (PMID: 29127046) Biotechnol Prog. 2012 May-Jun;28(3):780-7. (PMID: 22374883) Cytotechnology. 2018 Apr;70(2):503-512. (PMID: 28470539) FEBS Lett. 2001 Feb 16;490(3):171-8. (PMID: 11223032) J Sci Food Agric. 2021 Jan 15;101(1):7-14. (PMID: 32662148) Cytotechnology. 1999 Nov;31(3):221-4. (PMID: 19003145) Stem Cells Int. 2020 Jul 15;2020:9529465. (PMID: 32733574) Biotechnol Bioeng. 2021 Aug;118(8):3175-3186. (PMID: 34076888) Cytotechnology. 2020 Feb;72(1):111-120. (PMID: 31884572) J Cell Sci. 2006 May 1;119(Pt 9):1824-32. (PMID: 16608873) Methods Mol Biol. 2019;1889:255-266. (PMID: 30367419) Toxicol In Vitro. 2010 Jun;24(4):1053-63. (PMID: 20362047) Nat Food. 2022 Jan;3(1):74-85. (PMID: 37118488) EMBO J. 1999 Feb 15;18(4):926-33. (PMID: 10022835) NPJ Regen Med. 2020 May 11;5:10. (PMID: 32411395) J Biotechnol. 2001 Sep 13;91(1):63-74. (PMID: 11522363) Exp Cell Res. 1991 Aug;195(2):284-94. (PMID: 1649054) Meat Sci. 2012 Nov;92(3):297-301. (PMID: 22543115) Cell Tissue Res. 2013 Dec;354(3):671-82. (PMID: 23749200) Biotechnol Bioeng. 1996 Jun 20;50(6):627-35. (PMID: 18627071) Cell. 2007 Jun 1;129(5):999-1010. (PMID: 17540178) Tissue Eng Part B Rev. 2018 Oct;24(5):373-391. (PMID: 29652595) Int J Biochem Cell Biol. 2016 Oct;79:128-138. (PMID: 27590850) Sci Rep. 2018 Jul 17;8(1):10808. (PMID: 30018348) Front Nutr. 2020 Feb 20;7:10. (PMID: 32154261) Bioengineering (Basel). 2021 Jul 07;8(7):. (PMID: 34356203) |
| فهرسة مساهمة: | Keywords: bioreactor; microcarriers; skeletal muscle satellite cell; stem cell expansion; upscaling |
| تواريخ الأحداث: | Date Created: 20230823 Latest Revision: 20230824 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC10442166 |
| DOI: | 10.3389/fnut.2023.1192365 |
| PMID: | 37609488 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2296-861X |
|---|---|
| DOI: | 10.3389/fnut.2023.1192365 |