دورية أكاديمية
أعرض في EDS

Milk oligosaccharide-driven persistence of Bifidobacterium pseudocatenulatum modulates local and systemic microbial metabolites upon synbiotic treatment in conventionally colonized mice.

التفاصيل البيبلوغرافية
العنوان: Milk oligosaccharide-driven persistence of Bifidobacterium pseudocatenulatum modulates local and systemic microbial metabolites upon synbiotic treatment in conventionally colonized mice.
المؤلفون: Larke JA; Department of Nutrition, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA., Heiss BE; Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA., Ehrlich AM; Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, , Davis, CA, USA., Taft DH; Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA., Raybould HE; Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, , Davis, CA, USA., Mills DA; Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA. damills@ucdavis.edu., Slupsky CM; Department of Nutrition, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA. cslupsky@ucdavis.edu.; Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA. cslupsky@ucdavis.edu.
المصدر: Microbiome [Microbiome] 2023 Aug 28; Vol. 11 (1), pp. 194. Date of Electronic Publication: 2023 Aug 28.
نوع المنشور: Video-Audio Media; Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101615147 Publication Model: Electronic Cited Medium: Internet ISSN: 2049-2618 (Electronic) Linking ISSN: 20492618 NLM ISO Abbreviation: Microbiome Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London: BioMed Central, 2013-
مواضيع طبية MeSH: Bifidobacterium pseudocatenulatum* , Synbiotics* , Actinobacteria*, Humans ; Animals ; Mice ; RNA, Ribosomal, 16S/genetics ; Milk, Human ; Oligosaccharides ; Bifidobacterium ; Prebiotics
مستخلص: Background: Bifidobacteria represent an important gut commensal in humans, particularly during initial microbiome assembly in the first year of life. Enrichment of Bifidobacterium is mediated though the utilization of human milk oligosaccharides (HMOs), as several human-adapted species have dedicated genomic loci for transport and metabolism of these glycans. This results in the release of fermentation products into the gut lumen which may offer physiological benefits to the host. Synbiotic pairing of probiotic species with a cognate prebiotic delivers a competitive advantage, as the prebiotic provides a nutrient niche.
Methods: To determine the fitness advantage and metabolic characteristics of an HMO-catabolizing Bifidobacterium strain in the presence or absence of 2'-fucosyllactose (2'-FL), conventionally colonized mice were gavaged with either Bifidobacterium pseudocatenulatum MP80 (B.p. MP80) (as the probiotic) or saline during the first 3 days of the experiment and received water or water containing 2'-FL (as the prebiotic) throughout the study.
Results: 16S rRNA gene sequencing revealed that mice provided only B.p. MP80 were observed to have a similar microbiota composition as control mice throughout the experiment with a consistently low proportion of Bifidobacteriaceae present. Using 1 H NMR spectroscopy, similar metabolic profiles of gut luminal contents and serum were observed between the control and B.p. MP80 group. Conversely, synbiotic supplemented mice exhibited dramatic shifts in their community structure across time with an overall increased, yet variable, proportion of Bifidobacteriaceae following oral inoculation. Parsing the synbiotic group into high and moderate bifidobacterial persistence based on the median proportion of Bifidobacteriaceae, significant differences in gut microbial diversity and metabolite profiles were observed. Notably, metabolites associated with the fermentation of 2'-FL by bifidobacteria were significantly greater in mice with a high proportion of Bifidobacteriaceae in the gut suggesting metabolite production scales with population density. Moreover, 1,2-propanediol, a fucose fermentation product, was only observed in the liver and brain of mice harboring high proportions of Bifidobacteriaceae.
Conclusions: This study reinforces that the colonization of the gut with a commensal microorganism does not guarantee a specific functional output. Video Abstract.
(© 2023. BioMed Central Ltd., part of Springer Nature.)
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معلومات مُعتمدة: R01 AT008759 United States AT NCCIH NIH HHS; F32HD093185 United States NH NIH HHS; 5T32AI060555 United States NH NIH HHS
فهرسة مساهمة: Keywords: 2′-FL; Colonization; HMO; Human milk oligosaccharide; Mice; Prebiotic; Probiotic
المشرفين على المادة: 0 (RNA, Ribosomal, 16S)
0 (Oligosaccharides)
0 (Prebiotics)
تواريخ الأحداث: Date Created: 20230827 Date Completed: 20230829 Latest Revision: 20231122
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC10463478
DOI: 10.1186/s40168-023-01624-9
PMID: 37635250
قاعدة البيانات: MEDLINE
الوصف
تدمد:2049-2618
DOI:10.1186/s40168-023-01624-9