دورية أكاديمية
Effects of cadmium exposure on metabolism, antioxidant defense, immune function, and the hepatopancreas transcriptome of Cipangopaludina cathayensis.
| العنوان: | Effects of cadmium exposure on metabolism, antioxidant defense, immune function, and the hepatopancreas transcriptome of Cipangopaludina cathayensis. |
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| المؤلفون: | Wu YY; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin 541004, China., Tian WF; College of Biotechnology, Guilin Medical University, Guilin 541004, China., Cheng CX; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin 541004, China., Yang L; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin 541004, China., Ye QQ; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin 541004, China., Li WH; College of Animal Science and Technology, Guangxi University, Nanning 530004, China., Jiang JY; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin 541004, China; Guangxi Key Laboratory of Rare and Endangered Animal Ecology, Gangxi Normal University, Guilin 541004, China; Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China. Electronic address: jiangjy@mailbox.gxnu.edu.cn. |
| المصدر: | Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2023 Oct 01; Vol. 264, pp. 115416. Date of Electronic Publication: 2023 Aug 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7805381 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2414 (Electronic) Linking ISSN: 01476513 NLM ISO Abbreviation: Ecotoxicol Environ Saf Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Netherlands : Elsevier |
| مواضيع طبية MeSH: | Transcriptome* , Antioxidants*/metabolism, Animals ; Cadmium/metabolism ; Hepatopancreas/metabolism ; Immunity |
| مستخلص: | Cadmium (Cd) is a common contaminant in aquatic environments. However, little is known about the mechanisms underlying Cd toxicity in the freshwater snail Cipangopaludina cathayensis (Heude, 1890). This study to investigate the toxic effects of Cd on the standard metabolism, antioxidant activities, immune function, and hepatopancreas transcriptome profiles of C. cathayensis. C. cathayensis was exposed to 0.25, 0.5, 1.0, or 1.5 mg/L Cd for 3 h, with results showing that Cd significantly inhibited oxygen consumption and ammonia excretion and disrupted the respiratory metabolism of C. cathayensis. In addition, the O:N ratio dropped below 7, indicating that C. cathayensis may rely exclusively on proteins as an energy source under Cd stress. To understand how Cd impacts the antioxidant activities, immune function, and transcriptional profiles, C. cathayensis were exposed to 0.5 (low exposure, L14) or 1.5 (high exposure, H14) mg/L Cd for 14 days. Our results indicate that Cd exposure leads to oxidative stress and immunosuppression, with the latter effect being larger for exposure to higher Cd concentrations. A total of 2172 differentially expressed genes (DEGs) were identified by transcriptome analysis of the hepatopancreas, of which 885 were upregulated and 1287 were downregulated. Gene ontology and KEGG analyses revealed that the DEGs in the H14 group are enriched for energy generation terms and the "oxidative phosphorylation" pathway, respectively. Therefore, up-regulation of energy metabolism may be an adaptive strategy under Cd stress. Moreover, several genes involved in antioxidant activity were downregulated, whereas genes related to reactive oxygen species generation were upregulated. In addition, many immunity-related genes were identified within the DEGs, indicating that Cd toxicity may affect immune defense. Further, DEGs in the H14 group were enriched for disease-associated pathways. Taken together, our results indicate that Cd exposure leads to metabolic disorders, oxidative stress, and immunosuppression and thus may potentially contribute to disease outbreaks. Competing Interests: Declaration of Competing Interest The authors have declared that no conflict of interest exists. (Copyright © 2023. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: Cadmium; Cipangopaludina cathayensis; Oxidative stress; Standard metabolism; Transcriptome analysis |
| المشرفين على المادة: | 0 (Antioxidants) 00BH33GNGH (Cadmium) |
| تواريخ الأحداث: | Date Created: 20230830 Date Completed: 20231023 Latest Revision: 20231023 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.ecoenv.2023.115416 |
| PMID: | 37647801 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2414 |
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| DOI: | 10.1016/j.ecoenv.2023.115416 |