التفاصيل البيبلوغرافية
| العنوان: |
Aromatic natural products synthesis from aromatic lignin monomers using Acinetobacter baylyi ADP1. |
| المؤلفون: |
Biggs BW; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Tyo KEJ; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA. |
| المصدر: |
BioRxiv : the preprint server for biology [bioRxiv] 2023 Aug 24. Date of Electronic Publication: 2023 Aug 24. |
| نوع المنشور: |
Preprint |
| اللغة: |
English |
| بيانات الدورية: |
Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: |
Achieving sustainable chemical synthesis and a circular economy will require process innovation to minimize or recover existing waste streams. Valorization of lignin biomass has the ability to advance this goal. While lignin has proved a recalcitrant feedstock for upgrading, biological approaches can leverage native microbial metabolism to simplify complex and heterogeneous feedstocks to tractable starting points for biochemical upgrading. Recently, we demonstrated that one microbe with lignin relevant metabolism, Acinetobacter baylyi ADP1, is both highly engineerable and capable of undergoing rapid design-build-test-learn cycles, making it an ideal candidate for these applications. Here, we utilize these genetic traits and ADP1's native β-ketoadipate metabolism to convert mock alkali pretreated liquor lignin (APL) to two valuable natural products, vanillin-glucoside and resveratrol. En route, we create strains with up to 22 genetic modifications, including up to 8 heterologously expressed enzymes. Our approach takes advantage of preexisting aromatic species in APL (vanillate, ferulate, and p -coumarate) to create shortened biochemical routes to end products. Together, this work demonstrates ADP1's potential as a platform for upgrading lignin waste streams and highlights the potential for biosynthetic methods to maximize the existing chemical potential of lignin aromatic monomers.
Competing Interests: Conflict of Interest B.W.B. and K.E.J.T. have filed a patent (US Patent App. 17/681,434) for vanillin-glucoside synthesis with ADP1 from lignin. |
| معلومات مُعتمدة: |
T32 GM008449 United States GM NIGMS NIH HHS |
| تواريخ الأحداث: |
Date Created: 20230904 Latest Revision: 20231019 |
| رمز التحديث: |
20231215 |
| مُعرف محوري في PubMed: |
PMC10473687 |
| DOI: |
10.1101/2023.08.24.554694 |
| PMID: |
37662333 |
| قاعدة البيانات: |
MEDLINE |
