دورية أكاديمية
A high-throughput microfluidic mechanoporation platform to enable intracellular delivery of cyclic peptides in cell-based assays.
العنوان: | A high-throughput microfluidic mechanoporation platform to enable intracellular delivery of cyclic peptides in cell-based assays. |
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المؤلفون: | Kasper SH; Merck & Co., Inc. Cambridge Massachusetts USA., Otten S; Merck & Co., Inc. Cambridge Massachusetts USA., Squadroni B; Merck & Co., Inc. West Point Pennsylvania USA., Orr-Terry C; Merck & Co., Inc. Cambridge Massachusetts USA., Kuang Y; Merck & Co., Inc. Cambridge Massachusetts USA., Mussallem L; Merck & Co., Inc. West Point Pennsylvania USA., Ge L; Merck & Co., Inc. Kenilworth New Jersey USA., Yan L; Merck & Co., Inc. Kenilworth New Jersey USA., Kannan S; Agency for Science, Technology and Research (A*STAR) Singapore Singapore., Verma CS; Agency for Science, Technology and Research (A*STAR) Singapore Singapore., Brown CJ; Agency for Science, Technology and Research (A*STAR) Singapore Singapore., Johannes CW; Agency for Science, Technology and Research (A*STAR) Singapore Singapore., Lane DP; Agency for Science, Technology and Research (A*STAR) Singapore Singapore., Chandramohan A; MSD International Singapore Singapore., Partridge AW; MSD International Singapore Singapore., Roberts LR; Merck & Co., Inc. Cambridge Massachusetts USA., Josien H; Merck & Co., Inc. Kenilworth New Jersey USA., Therien AG; Merck & Co., Inc. Cambridge Massachusetts USA., Hett EC; Merck & Co., Inc. Cambridge Massachusetts USA., Howell BJ; Merck & Co., Inc. West Point Pennsylvania USA., Peier A; Merck & Co., Inc. Kenilworth New Jersey USA., Ai X; Merck & Co., Inc. Kenilworth New Jersey USA., Cassaday J; Merck & Co., Inc. West Point Pennsylvania USA. |
المصدر: | Bioengineering & translational medicine [Bioeng Transl Med] 2023 May 13; Vol. 8 (5), pp. e10542. Date of Electronic Publication: 2023 May 13 (Print Publication: 2023). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: John Wiley & Sons Inc Country of Publication: United States NLM ID: 101689146 Publication Model: eCollection Cited Medium: Print ISSN: 2380-6761 (Print) Linking ISSN: 23806761 NLM ISO Abbreviation: Bioeng Transl Med Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Hoboken, NJ : John Wiley & Sons Inc., [2016]- |
مستخلص: | Cyclic peptides are poised to target historically difficult to drug intracellular protein-protein interactions, however, their general cell impermeability poses a challenge for characterizing function. Recent advances in microfluidics have enabled permeabilization of the cytoplasmic membrane by physical cell deformation (i.e., mechanoporation), resulting in intracellular delivery of impermeable macromolecules in vector- and electrophoretic-free approaches. However, the number of payloads (e.g., peptides) and/or concentrations delivered via microfluidic mechanoporation is limited by having to pre-mix cells and payloads, a manually intensive process. In this work, we show that cells are momentarily permeable ( t Competing Interests: All authors that are present or former employees of Merck Sharp & Dohme LLC, a subsidiary of Merck & Co., Inc., Rahway, NJ or MSD International, Singapore may hold stocks and/or stock options in Merck & Co., Inc., Rahway, NJ. Srinivasaraghavan Kannan and Chandra S. Verma are co‐founders of Sinopsee Therapeutics and Aplomex; the current work does not have a conflict. (© 2023 Merck Sharp & Dohme LLC and The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers.) |
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فهرسة مساهمة: | Keywords: automation; cell‐based assays; cyclic peptides; intracellular delivery; microfluidics; protein–protein interactions |
تواريخ الأحداث: | Date Created: 20230911 Latest Revision: 20230913 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC10487316 |
DOI: | 10.1002/btm2.10542 |
PMID: | 37693049 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2380-6761 |
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DOI: | 10.1002/btm2.10542 |