دورية أكاديمية
Co-administration of Thymoquinone and Propolis in Liposomal Formulations as a Potential Approach for Treatment of Acetic Acid-Induced Ulcerative Colitis: Physiological and Histopathological Analysis.
| العنوان: | Co-administration of Thymoquinone and Propolis in Liposomal Formulations as a Potential Approach for Treatment of Acetic Acid-Induced Ulcerative Colitis: Physiological and Histopathological Analysis. |
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| المؤلفون: | Alaaeldin E; Department of Pharmaceutics, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt. eman_alaa_eldin@yahoo.com.; Department of Pharmaceutics, Faculty of Pharmacy, Deraya University, Minia, 61111, Egypt. eman_alaa_eldin@yahoo.com., Refaat H; Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA, 52264, USA., Saber EA; Department of Medicine, Deraya University, New Minia City, Egypt., Aziz NM; Department of Medicine, Deraya University, New Minia City, Egypt., Abdel-Maqsoud NMR; Department of Medicine, Deraya University, New Minia City, Egypt., Aleem MMAE; Department of Medicine, Deraya University, New Minia City, Egypt., Kamel MY; Department of Pharmacology, Faculty of Medicine, Minia University, Minia, Egypt., Mady FM; Department of Pharmaceutics, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt. |
| المصدر: | AAPS PharmSciTech [AAPS PharmSciTech] 2023 Sep 19; Vol. 24 (7), pp. 190. Date of Electronic Publication: 2023 Sep 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: United States NLM ID: 100960111 Publication Model: Electronic Cited Medium: Internet ISSN: 1530-9932 (Electronic) Linking ISSN: 15309932 NLM ISO Abbreviation: AAPS PharmSciTech Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York : Springer Original Publication: Arlington, VA : American Association of Pharmaceutical Scientists, c2000- |
| مواضيع طبية MeSH: | Colitis, Ulcerative*/chemically induced , Colitis, Ulcerative*/drug therapy , Propolis* , Colitis*/chemically induced , Colitis*/drug therapy, Humans ; Acetic Acid ; Liposomes ; Inflammation |
| مستخلص: | A severe form of autoimmune-mediated inflammatory bowel disease (IBD) is termed as ulcerative colitis (UC) which ultimately results in significant mucosal damage and ulceration. Herbal remedies may be employed as an alternative for treatment of UC instead of conventional medications such as Sulfasalazine. Promising natural remedies for the treatment of IBD, including colitis, are propolis extract (PP) and thymoquinone (TQ). This study is aimed at assessing the potential of liposomal formulations of TQ and Egyptian PP in combination therapy on improving their therapeutic efficacy against ulcerative colitis in order to maximize the potential of their beneficial clinical effects. Clinical, biochemical, and histological evaluations of colonic mucosal damage and inflammation were evaluated. The results exhibited a significant increase in tissue MDA, TNFα, and nitrite levels with activation of caspase-3 in the acetic acid-induced colitis group, which is predominantly downregulated in the treatment groups. The prepared formulations of TQ and PP revealed liposomal vesicles in a nanoscale size (192 ± 20.3 and 98.2 ± 20.3 nm, respectively) and accepted stability indicated with a zeta potential of 19.3 ± 0.11 and 17.1 ± 0.25 mV, respectively. They showed an entrapment efficiency of 85.3 ± 12.6% and 69.3 ± 11.8%, respectively. At comparable doses, combination therapy with thymoquinone liposomes and propolis liposomes considerably outperformed free TQ and free PP in reducing inflammation of UC as shown in the present study by clinical, biochemical, and histological evaluations. (© 2023. The Author(s).) |
| References: | Al-Rejaie SS, Abuohashish HM, Al-Enazi MM, Al-Assaf AH, Parmar MY, Ahmed MM. Protective effect of naringenin on acetic acid-induced ulcerative colitis in rats. World J Gastroenterol. 2013;19:5633–44. (PMID: 24039355376989910.3748/wjg.v19.i34.5633) Chu TPC, Moran GW, Card TR. The pattern of underlying cause of death in patients with inflammatory bowel disease in England: a record linkage study. J Crohns Colitis. 2017;11:578–85. (PMID: 28453767) Sun P, Zhang S. Correlations of 25-hydroxyvitamin D3 level in patients with ulcerative colitis with inflammation level, immunity and disease activity. Eur Rev Med Pharmacol Sci. 2018;22:5635–9. (PMID: 30229839) Helal MG, Abd Elhameed AG. Graviola mitigates acetic acid–induced ulcerative colitis in rats: insight on apoptosis and Wnt/Hh signaling crosstalk. Environ Sci Pollut Res. 2021;28:29615–28. (PMID: 10.1007/s11356-021-12716-0) Pecere S, Petito V, Amato A, Poscia A, Armuzzi A, Lopetuso LR, Sgambato A, Cammarota G, Papa A, Gasbarrini A. Infliximab and tumour necrosis factor alpha measurement on intestinal mucosa: a new tool for the clinic? Emj Gastroenterol. 2016;59:107–15. (PMID: 10.33590/emjgastroenterol/10310965) El-Akabawy G, El-Sherif NM. Zeaxanthin exerts protective effects on acetic acid-induced colitis in rats via modulation of pro-inflammatory cytokines and oxidative stress. Biomed Pharmacother. 2019;111:841–51. (PMID: 3061608310.1016/j.biopha.2019.01.001) Ansari MN, Rehman NU, Karim A, Soliman GA, Ganaie MA, Raish M, Hamad AM. Role of oxidative stress and inflammatory cytokines (TNF-α and IL-6) in acetic acid-induced ulcerative colitis in rats: ameliorated by Otostegia fruticosa. Life. 2021;11:195. (PMID: 33802553800114810.3390/life11030195) Bankova VS, de Castro SL, Marcucci MC. Propolis: recent advances in chemistry and plant origin. Apidologie. 2000;31:3–15. (PMID: 10.1051/apido:2000102) Hwang S, Hwang S, Jo M, Lee CG, Rhee K-J. Oral administration of Korean propolis extract ameliorates DSS-induced colitis in BALB/c mice. Int J Med Sci. 2020;17:1984. (PMID: 32788877741539710.7150/ijms.44834) Silva LMd, Souza Pd, Jaouni SKA, Harakeh S, Golbabapour S, de Andrade SF. Propolis and its potential to treat gastrointestinal disorders. Evid-Based Complement Altern Med. 2018;2018:1–12. Mariano LNB, Arruda C, Somensi LB, Costa APM, Perondi EG, Boeing T, Mariott M, da Silva RdCMVd, Fonseca A, de Souza P. Brazilian green propolis hydroalcoholic extract reduces colon damages caused by dextran sulfate sodium-induced colitis in mice. Inflammopharmacology. 2018;26:1283–92. (PMID: 2963310410.1007/s10787-018-0467-z) Wang K, Jin X, Li Q, Sawaya ACHF, Le Leu RK, Conlon MA, Wu L, Hu F. Propolis from different geographic origins decreases intestinal inflammation and Bacteroides spp. populations in a model of DSS-Induced colitis. Molec Nutr Food Res. 2018;62:1800080. (PMID: 10.1002/mnfr.201800080) Samak YO, Santhanes D, El-Massik MA, Coombes AG. Formulation strategies for achieving high delivery efficiency of thymoquinone-containing Nigella sativa extract to the colon based on oral alginate microcapsules for treatment of inflammatory bowel disease. J Microencapsul. 2019;36:204–14. (PMID: 3116402710.1080/02652048.2019.1620356) Lei X, Liu M, Yang Z, Ji M, Guo X, Dong W. Thymoquinone prevents and ameliorates dextran sulfate sodium-induced colitis in mice. Dig Dis Sci. 2012;57:2296–303. (PMID: 2247658810.1007/s10620-012-2156-x) Mahgoub AA. Thymoquinone protects against experimental colitis in rats. Toxicol Lett. 2003;143:133–43. (PMID: 1274981710.1016/S0378-4274(03)00173-5) Salmani JMM, Asghar S, Lv H, Zhou J. Aqueous solubility and degradation kinetics of the phytochemical anticancer thymoquinone; probing the effects of solvents, pH and light. Molecules. 2014;19:5925–39. (PMID: 24815311627077010.3390/molecules19055925) Alaaeldin E, Mostafa M, Mansour HF, Soliman GM. Spanlastics as an efficient delivery system for the enhancement of thymoquinone anticancer efficacy: fabrication and cytotoxic studies against breast cancer cell lines. J Drug Deliv Sci Technol. 2021;65: 102725. (PMID: 10.1016/j.jddst.2021.102725) Elkot M, Elsayed M, Alaaeldin E, Sarhan H, Shaykoon MSA, Elsadek B. Accelerated stability testing of microcapsulated sorafenib-loaded carbon nanotubes prepared by emulsification/internal gelation method. Int J Pharm Pharm Res. 2019;16:126–39. Refaat H, Naguib YW, Elsayed MM, Sarhan HA, Alaaeldin E. Modified spraying technique and response surface methodology for the preparation and optimization of propolis liposomes of enhanced anti-proliferative activity against human melanoma cell line A375. Pharmaceutics. 2019;11:558. (PMID: 31661945692104210.3390/pharmaceutics11110558) Mostafa M, Alaaeldin E, Aly UF, Sarhan HA. Optimization and characterization of thymoquinone-loaded liposomes with enhanced topical anti-inflammatory activity. AAPS PharmSciTech. 2018;19:3490–500. (PMID: 3021826510.1208/s12249-018-1166-1) Musa A, Elmaidomy AH, Sayed AM, Alzarea SI, Al-Sanea MM, Mostafa EM, Hendawy OM, Abdelgawad MA, Youssif KA, Refaat H. Cytotoxic potential, metabolic profiling, and liposomes of Coscinoderma sp. crude extract supported by in silico analysis. Int J Nanomed. 2021;16:3861. (PMID: 10.2147/IJN.S310720) Ahmad A, Vaghasiya K, Kumar A, Alam P, Raza SS, Verma RK, Khan R. Enema based therapy using liposomal formulation of low molecular weight heparin for treatment of active ulcerative colitis: new adjunct therapeutic opportunity. Mater Sci Eng, C. 2021;121: 111851. (PMID: 10.1016/j.msec.2020.111851) Tang Q, Zhang W, Zhang C, Guan Y, Ding J, Yuan C, Tan C, Gao X, Tan S. Oxymatrine loaded nitric oxide-releasing liposomes for the treatment of ulcerative colitis. Int J Pharm. 2020;586: 119617. (PMID: 3265011110.1016/j.ijpharm.2020.119617) Wang C, Han Z, Wu Y, Lu X, Tang X, Xiao J, Li N. Enhancing stability and anti-inflammatory properties of curcumin in ulcerative colitis therapy using liposomes mediated colon-specific drug delivery system. Food Chem Toxicol. 2021;151: 112123. (PMID: 3374437910.1016/j.fct.2021.112123) Xian J, Zhong X, Gu H, Wang X, Li J, Li J, Wu Y, Zhang C, Zhang J. Colonic delivery of celastrol-loaded layer-by-layer liposomes with pectin/trimethylated chitosan coating to enhance its anti-ulcerative colitis effects. Pharmaceutics. 2021;13:2005. (PMID: 34959287870335410.3390/pharmaceutics13122005) Refaat H, Mady FM, Sarhan HA, Rateb HS, Alaaeldin E. Optimization and evaluation of propolis liposomes as a promising therapeutic approach for COVID-19. Int J Pharm. 2021;592: 120028. (PMID: 3316658410.1016/j.ijpharm.2020.120028) Odeh F, Ismail SI, Abu-Dahab R, Mahmoud IS, Al Bawab A. Thymoquinone in liposomes: a study of loading efficiency and biological activity towards breast cancer. Drug Delivery. 2012;19:371–7. (PMID: 2304362610.3109/10717544.2012.727500) Lopez-Pinto J, Gonzalez-Rodriguez M, Rabasco A. Effect of cholesterol and ethanol on dermal delivery from DPPC liposomes. Int J Pharm. 2005;298:1–12. (PMID: 1589693210.1016/j.ijpharm.2005.02.021) New RR (1990) Preparation of liposomes. Liposomes: a practical approach, 33–104. Ruozi B, et al. Atomic force microscopy and photon correlation spectroscopy: two techniques for rapid characterization of liposomes. Eur J Pharm Sci. 2005;25:81–9. Shin M-R, Kim KJ, Kim SH, Kim SJ, Seo B-I, An H-J, Roh S-S. Comparative evaluation between sulfasalazine alone and in combination with herbal medicine on DSS-induced ulcerative colitis mice. BioMed Res Int. 2017;2017:1–10. Aslan A, Temiz M, Atik E, Polat G, Sahinler N, Besirov E, Aban N, Parsak CK. Effectiveness of mesalamine and propolis in experimental colitis. Adv Ther. 2007;24:1085–97. (PMID: 1802933610.1007/BF02877715) Niu X, Zhang H, Li W, Wang Y, Mu Q, Wang X, He Z, Yao H. Protective effect of cavidine on acetic acid-induced murine colitis via regulating antioxidant, cytokine profile and NF-κB signal transduction pathways. Chem Biol Interact. 2015;239:34–45. (PMID: 2610200910.1016/j.cbi.2015.06.026) Kannan N, Guruvayoorappan C. Protective effect of Bauhinia tomentosa on acetic acid induced ulcerative colitis by regulating antioxidant and inflammatory mediators. Int Immunopharmacol. 2013;16:57–66. (PMID: 2353802510.1016/j.intimp.2013.03.008) Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95:351–8. (PMID: 3681010.1016/0003-2697(79)90738-3) Drury R, Wallington E. Carleton’s histological technique. 5th ed. New York: Churchill Livingstone; 1980. Bancroft JD, Gamble M (2008) Theory and practice of histological techniques (Elsevier health sciences). Abou-Taleb HA, Sayed AM, Refaat H, Alsenani F, Alaaeldin E, Mokhtar FA, Abdelmohsen UR, Shady NH. Network pharmacological analysis of the red sea sponge hyrtios erectus extract to reveal anticancer efficacy of corresponding loaded niosomes. Mar Drugs. 2022;20:628. (PMID: 36286452960479410.3390/md20100628) Musa A, Elmaidomy AH, Sayed AM, Alzarea SI, Al-Sanea MM, Mostafa EM, Hendawy OM, Abdelgawad MA, Youssif KA, Refaat H. Cytotoxic potential, metabolic profiling, and liposomes of Coscinoderma sp. crude extract supported by in silico analysis. Int J Nanomed. 2021;16:3861–74. (PMID: 10.2147/IJN.S310720) Sadraei H, Asghari G, Khanabadi M, Minaiyan M. Anti-inflammatory effect of apigenin and hydroalcoholic extract of Dracocephalum kotschyi on acetic acid-induced colitis in rats. Res Pharm Sci. 2017;12:322–9. (PMID: 28855944556600710.4103/1735-5362.212050) Kandhare A, Patil A, Guru A, Mukhrjee A, Sarkar A, Sengupta A, Parmar H, Muthal A, Wangikar P, Bodhankar S. Ameliorative effect of ferulic acid against acetic acid induced ulcerative colitis: role of HO-1 and Nrf2. Pharmacologia. 2016;7:114–24. (PMID: 10.5567/pharmacologia.2016.114.124) Colombo BB, Fattori V, Guazelli CFS, Zaninelli TH, Carvalho TT, Ferraz CR, Bussmann AJC, Ruiz-Miyazawa KW, Baracat MM, Casagrande R, et al. Vinpocetine ameliorates acetic acid-induced colitis by inhibiting NF-κB activation in mice. Inflammation. 2018;41:1276–89. (PMID: 2963310310.1007/s10753-018-0776-9) Hunschede S, Kubant R, Akilen R, Thomas S, Anderson GH. Decreased appetite after high-intensity exercise correlates with increased plasma interleukin-6 in normal-weight and overweight/obese boys. Curr Dev Nutr. 2017;1: e000398. (PMID: 29955695599891510.3945/cdn.116.000398) Joo M, Kim HS, Kwon TH, Palikhe A, Zaw TS, Jeong JH, Sohn UD. Anti-inflammatory effects of flavonoids on TNBS-induced colitis of rats. KJPP. 2014;19:43–50. DeRoche TC, Xiao S-Y, Liu X. Histological evaluation in ulcerative colitis. Gastroenterol Rep. 2014;2:178–92. (PMID: 10.1093/gastro/gou031) Ismail DI, Aboulkhair AG. Royal jelly protects against experimentally-induced ulcerative colitis in adult male albino rats: a histological study. Egypt J Histol. 2018;41:192–203. (PMID: 10.21608/EJH.2018.13841) Kuo W-T, Shen L, Zuo L, Shashikanth N, Ong MLDM, Wu L, Zha J, Edelblum KL, Wang Y, Wang Y. Inflammation-induced occludin downregulation limits epithelial apoptosis by suppressing caspase-3 expression. Gastroenterology. 2019;157:1323–37. (PMID: 3140114310.1053/j.gastro.2019.07.058) Yang L, Shen L, Li Y, Li Y, Yu S, Wang S. Hyperoside attenuates dextran sulfate sodium-induced colitis in mice possibly via activation of the Nrf2 signalling pathway. J Inflamm. 2017;14:1–10. (PMID: 10.1186/s12950-017-0172-5) |
| فهرسة مساهمة: | Keywords: anti-inflammation; liposomes; propolis; thymoquinone; ulcerative colitis |
| المشرفين على المادة: | Q40Q9N063P (Acetic Acid) 9009-62-5 (Propolis) O60IE26NUF (thymoquinone) 0 (Liposomes) |
| تواريخ الأحداث: | Date Created: 20230919 Date Completed: 20230921 Latest Revision: 20231024 |
| رمز التحديث: | 20231024 |
| DOI: | 10.1208/s12249-023-02637-1 |
| PMID: | 37726590 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1530-9932 |
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| DOI: | 10.1208/s12249-023-02637-1 |