دورية أكاديمية
أعرض في EDS

Identification and immunogenic potential of glycosylphosphatidylinositol-anchored proteins in Paracoccidioides brasiliensis .

التفاصيل البيبلوغرافية
العنوان: Identification and immunogenic potential of glycosylphosphatidylinositol-anchored proteins in Paracoccidioides brasiliensis .
المؤلفون: Gonçales RA; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.; ICVS/3B's - PT Government Associate Laboratory, Braga, Portugal., Portis IG; Laboratorio de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil., Dos Reis TF; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil., Basso Júnior LR; Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Ribeirão Preto Medical School, Universidade de São Paulo (FMRP/USP), Ribeirão Preto, SP, Brazil., Martinez R; Departamento de Clínica Médica, Faculdade de Medicina de Ribeirão Preto (FMRP), Universidade de São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Zhu H; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, United States., Pereira M; Laboratorio de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil., Soares CMA; Laboratorio de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil., Coelho PSR; Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Ribeirão Preto Medical School, Universidade de São Paulo (FMRP/USP), Ribeirão Preto, SP, Brazil.
المصدر: Frontiers in fungal biology [Front Fungal Biol] 2023 Aug 29; Vol. 4, pp. 1243475. Date of Electronic Publication: 2023 Aug 29 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 9918266300506676 Publication Model: eCollection Cited Medium: Internet ISSN: 2673-6128 (Electronic) Linking ISSN: 26736128 NLM ISO Abbreviation: Front Fungal Biol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media S.A., [2020]-
مستخلص: In fungal pathogens the cell wall plays an important role in host-pathogen interactions because its molecular components (e.g., polysaccharides and proteins) may trigger immune responses during infection. GPI-anchored proteins represent the main protein class in the fungal cell wall where they can perform several functions, such as cell wall remodeling and adhesion to host tissues. Genomic analysis has identified the complement of GPI-anchored proteins in many fungal pathogens, but the function has remained unknown for most of them. Here, we conducted an RNA expression analysis of GPI-anchored proteins of Paracoccidioides brasiliensis which causes paracoccidioidomycosis (PCM), an important human systemic mycosis endemic in Latin America. The expression of the GPI-anchored proteins was analyzed by quantitative PCR in both the mycelium and yeast forms. qPCR analysis revealed that the transcript levels of 22 of them were increased in hyphae and 10 in yeasts, respectively, while 14 did not show any significant difference in either form. Furthermore, we cloned 46 open reading frames and purified their corresponding GPI-anchored proteins in the budding yeast. Immunoblot and ELISA analysis of four purified GPI-anchored proteins revealed immune reactivity of these proteins against sera obtained from PCM patients. The information obtained in this study provides valuable information about the expression of many GPI-anchored proteins of unknown function. In addition, based on our immune analysis, some GPI-anchored proteins are expressed during infection and therefore, they might serve as good candidates for the development of new diagnostic methods.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Gonçales, Portis, dos Reis, Basso Júnior, Martinez, Zhu, Pereira, Soares and Coelho.)
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فهرسة مساهمة: Keywords: GPI-anchored proteins; PCM; Paracoccidioides brasiliensis; Western blot; cell wall
تواريخ الأحداث: Date Created: 20230925 Latest Revision: 20230926
رمز التحديث: 20230926
مُعرف محوري في PubMed: PMC10512324
DOI: 10.3389/ffunb.2023.1243475
PMID: 37746134
قاعدة البيانات: MEDLINE
الوصف
تدمد:2673-6128
DOI:10.3389/ffunb.2023.1243475