دورية أكاديمية
High-Intensity Interval Training Ameliorates High-Fat Diet-Induced Metabolic Disorders via the Cyclic GMP-AMP Synthase-Stimulator of Interferon Gene Signaling Pathway.
العنوان: | High-Intensity Interval Training Ameliorates High-Fat Diet-Induced Metabolic Disorders via the Cyclic GMP-AMP Synthase-Stimulator of Interferon Gene Signaling Pathway. |
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المؤلفون: | Hu Z; Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, East China Normal University, Shanghai 200241, China.; College of Physical Education and Health, East China Normal University, Shanghai 200241, China., Li X; Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, East China Normal University, Shanghai 200241, China.; College of Physical Education and Health, East China Normal University, Shanghai 200241, China., Yang Y; Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, East China Normal University, Shanghai 200241, China.; College of Physical Education and Health, East China Normal University, Shanghai 200241, China., Zhang Z; Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, East China Normal University, Shanghai 200241, China.; College of Physical Education and Health, East China Normal University, Shanghai 200241, China., Ding S; Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, East China Normal University, Shanghai 200241, China.; College of Physical Education and Health, East China Normal University, Shanghai 200241, China. |
المصدر: | International journal of molecular sciences [Int J Mol Sci] 2023 Sep 08; Vol. 24 (18). Date of Electronic Publication: 2023 Sep 08. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
مواضيع طبية MeSH: | High-Intensity Interval Training* , Metabolic Diseases*/etiology , Metabolic Diseases*/therapy, Mice ; Animals ; Male ; Diet, High-Fat/adverse effects ; Mice, Inbred C57BL ; Signal Transduction/physiology ; Nucleotidyltransferases/genetics ; Nucleotidyltransferases/metabolism ; Interferons |
مستخلص: | Metabolic diseases are growing in prevalence worldwide. Although the pathogenesis of metabolic diseases remains ambiguous, the correlation between cyclic GMP-AMP synthase (cGAS)-stimulator of interferon gene (STING) and metabolic diseases has been identified recently. Exercise is an effective intervention protecting against metabolic diseases, however, the role of the cGAS-STING signaling pathway in this process is unclear, and the effect and mechanism of different exercise intensities on metabolic disorders are still unknown. Thus, we explored the association between exercise to ameliorate HFD-induced metabolic disorders and the cGAS-STING signaling pathway and compared the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT). Male C57BL/6 mice (6-8 weeks old) were fed HFD for 8 weeks to establish a metabolic disease model and were subjected to 8-week MICT or HIIT training. Glucose tolerance tests (GTT) and insulin tolerance tests (ITT) were used to assess glucose metabolism. Serum triglyceride (TG) and total cholesterol (TC) were measured to evaluate lipid metabolism. Oil red staining was used to observe the lipid droplets in the gastrocnemius muscle. An enzyme-linked immunosorbent assay was used to detect the serum inflammatory factors IL-6 and IFN-β. The protein expression of the cGAS-STING signaling pathway was detected by the Wes TM automatic protein expression analysis system. We reported that HFD induced metabolic disorders with obesity, abnormal glucolipid metabolism, and significant inflammatory responses. Both HIIT and MICT ameliorated the above adverse reactions, but MICT was superior to HIIT in improving glucolipid disorders. Additionally, HIIT significantly increased the expression of STING protein, as well as the phosphorylation of TBKI and the ratio of p-IRF3/IRF3. MICT only increased the expression of STING protein. Our findings suggest that HIIT may alleviate HFD-induced metabolic disorder phenotype through the cGAS-STING signaling pathway. However, the improvement of MICT on metabolic disorder phenotype is less associated with the cGAS-STING pathway, which needs to be further explored. |
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معلومات مُعتمدة: | 31671241, 32000836 National Natural Science Foundation of China |
فهرسة مساهمة: | Keywords: cGAS-STING signaling pathway; high-fat diet; high-intensity interval training; metabolic disorders; moderate-intensity continuous training |
المشرفين على المادة: | 0 (cyclic guanosine monophosphate-adenosine monophosphate) EC 2.7.7.- (Nucleotidyltransferases) 9008-11-1 (Interferons) |
تواريخ الأحداث: | Date Created: 20230928 Date Completed: 20231023 Latest Revision: 20231023 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC10531371 |
DOI: | 10.3390/ijms241813840 |
PMID: | 37762143 |
قاعدة البيانات: | MEDLINE |
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