دورية أكاديمية
Rhs NADase effectors and their immunity proteins are exchangeable mediators of inter-bacterial competition in Serratia.
| العنوان: | Rhs NADase effectors and their immunity proteins are exchangeable mediators of inter-bacterial competition in Serratia. |
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| المؤلفون: | Hagan M; School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK., Pankov G; School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK., Gallegos-Monterrosa R; School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK., Williams DJ; School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK., Earl C; School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK., Buchanan G; School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK., Hunter WN; School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK. w.n.hunter@dundee.ac.uk., Coulthurst SJ; School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK. s.j.coulthurst@dundee.ac.uk. |
| المصدر: | Nature communications [Nat Commun] 2023 Sep 28; Vol. 14 (1), pp. 6061. Date of Electronic Publication: 2023 Sep 28. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : Nature Pub. Group |
| مواضيع طبية MeSH: | Serratia*/genetics , Type VI Secretion Systems*/genetics , Type VI Secretion Systems*/metabolism, NAD+ Nucleosidase/genetics ; NAD+ Nucleosidase/metabolism ; Bacterial Proteins/metabolism ; Serratia marcescens/metabolism |
| مستخلص: | Many bacterial species use Type VI secretion systems (T6SSs) to deliver anti-bacterial effector proteins into neighbouring bacterial cells, representing an important mechanism of inter-bacterial competition. Specific immunity proteins protect bacteria from the toxic action of their own effectors, whilst orphan immunity proteins without a cognate effector may provide protection against incoming effectors from non-self competitors. T6SS-dependent Rhs effectors contain a variable C-terminal toxin domain (CT), with the cognate immunity protein encoded immediately downstream of the effector. Here, we demonstrate that Rhs1 effectors from two strains of Serratia marcescens, the model strain Db10 and clinical isolate SJC1036, possess distinct CTs which both display NAD(P) + glycohydrolase activity but belong to different subgroups of NADase from each other and other T6SS-associated NADases. Comparative structural analysis identifies conserved functions required for NADase activity and reveals that unrelated NADase immunity proteins utilise a common mechanism of effector inhibition. By replicating a natural recombination event, we show successful functional exchange of CTs and demonstrate that Db10 encodes an orphan immunity protein which provides protection against T6SS-delivered SJC1036 NADase. Our findings highlight the flexible use of Rhs effectors and orphan immunity proteins during inter-strain competition and the repeated adoption of NADase toxins as weapons against bacterial cells. (© 2023. Springer Nature Limited.) |
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| معلومات مُعتمدة: | United Kingdom WT_ Wellcome Trust; 215599/Z/19/Z United Kingdom WT_ Wellcome Trust; 104556/Z/14/Z United Kingdom WT_ Wellcome Trust; 220321/Z/20/Z United Kingdom WT_ Wellcome Trust; MR/N013735/1 United Kingdom MRC_ Medical Research Council |
| المشرفين على المادة: | EC 3.2.2.5 (NAD+ Nucleosidase) 0 (Bacterial Proteins) 0 (Type VI Secretion Systems) |
| تواريخ الأحداث: | Date Created: 20230928 Date Completed: 20231023 Latest Revision: 20231118 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC10539506 |
| DOI: | 10.1038/s41467-023-41751-3 |
| PMID: | 37770429 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 2041-1723 |
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| DOI: | 10.1038/s41467-023-41751-3 |