دورية أكاديمية
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Global transcriptome profiling reveals root- and leaf-specific responses of barley ( Hordeum vulgare L.) to H 2 O 2 .

التفاصيل البيبلوغرافية
العنوان: Global transcriptome profiling reveals root- and leaf-specific responses of barley ( Hordeum vulgare L.) to H 2 O 2 .
المؤلفون: Bhattacharyya S; Institute for Cellular and Molecular Botany, University of Bonn, Bonn, Germany., Giridhar M; Institute for Cellular and Molecular Botany, University of Bonn, Bonn, Germany.; Leibniz Institute for Food Systems Biology at the Technical University of Munich, Freising, Germany., Meier B; Institute of Agricultural and Nutritional Sciences, Faculty of Natural Sciences III, Martin Luther University Halle-Wittenberg, Halle, Germany., Peiter E; Institute of Agricultural and Nutritional Sciences, Faculty of Natural Sciences III, Martin Luther University Halle-Wittenberg, Halle, Germany., Vothknecht UC; Institute for Cellular and Molecular Botany, University of Bonn, Bonn, Germany., Chigri F; Institute for Cellular and Molecular Botany, University of Bonn, Bonn, Germany.
المصدر: Frontiers in plant science [Front Plant Sci] 2023 Sep 12; Vol. 14, pp. 1223778. Date of Electronic Publication: 2023 Sep 12 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101568200 Publication Model: eCollection Cited Medium: Print ISSN: 1664-462X (Print) Linking ISSN: 1664462X NLM ISO Abbreviation: Front Plant Sci Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Research Foundation, 2010-
مستخلص: In cereal crops, such as barley ( Hordeum vulgare L.), the ability to appropriately respond to environmental cues is an important factor for yield stability and thus for agricultural production. Reactive oxygen species (ROS), such as hydrogen peroxide (H 2 O 2 ), are key components of signal transduction cascades involved in plant adaptation to changing environmental conditions. H 2 O 2 -mediated stress responses include the modulation of expression of stress-responsive genes required to cope with different abiotic and biotic stresses. Despite its importance, knowledge of the effects of H 2 O 2 on the barley transcriptome is still scarce. In this study, we identified global transcriptomic changes induced after application of 10 mM H 2 O 2 to five-day-old barley plants. In total, 1883 and 1001 differentially expressed genes (DEGs) were identified in roots and leaves, respectively. Most of these DEGs were organ-specific, with only 209 DEGs commonly regulated and 37 counter-regulated between both plant parts. A GO term analysis further confirmed that different processes were affected in roots and leaves. It revealed that DEGs in leaves mostly comprised genes associated with hormone signaling, response to H 2 O 2 and abiotic stresses. This includes many transcriptions factors and small heat shock proteins. DEGs in roots mostly comprised genes linked to crucial aspects of H 2 O 2 catabolism and oxidant detoxification, glutathione metabolism, as well as cell wall modulation. These categories include many peroxidases and glutathione transferases. As with leaves, the H 2 O 2 response category in roots contains small heat shock proteins, however, mostly different members of this family were affected and they were all regulated in the opposite direction in the two plant parts. Validation of the expression of the selected commonly regulated DEGs by qRT-PCR was consistent with the RNA-seq data. The data obtained in this study provide an insight into the molecular mechanisms of oxidative stress responses in barley, which might also play a role upon other stresses that induce oxidative bursts.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Bhattacharyya, Giridhar, Meier, Peiter, Vothknecht and Chigri.)
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فهرسة مساهمة: Keywords: H2O2; RNA-sequencing; barley; oxidative stress; reactive oxygen species (ROS); stress response; transcriptome profiling
تواريخ الأحداث: Date Created: 20230929 Latest Revision: 20231003
رمز التحديث: 20231003
مُعرف محوري في PubMed: PMC10523330
DOI: 10.3389/fpls.2023.1223778
PMID: 37771486
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-462X
DOI:10.3389/fpls.2023.1223778