دورية أكاديمية
A SERS-based immune-nanoprobe for ultrasensitive detection of glycinin via a lateral flow assay.
| العنوان: | A SERS-based immune-nanoprobe for ultrasensitive detection of glycinin via a lateral flow assay. |
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| المؤلفون: | Huang P; College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, Henan, China., Yang S; College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, Henan, China., Chen Z; College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, Henan, China., Shang A; College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, Henan, China., Liu D; College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, Henan, China., Xi J; College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, Henan, China. Electronic address: xijunhnu@163.com. |
| المصدر: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2024 Jan 15; Vol. 305, pp. 123439. Date of Electronic Publication: 2023 Sep 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 9602533 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3557 (Electronic) Linking ISSN: 13861425 NLM ISO Abbreviation: Spectrochim Acta A Mol Biomol Spectrosc Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Original Publication: [Kidlington, Oxford, U.K. ; Tarrytown, NY] : Pergamon, c1994- |
| مواضيع طبية MeSH: | Gold*/chemistry , Metal Nanoparticles*/chemistry, Humans ; Animals ; Rabbits ; Antibodies ; Spectrum Analysis, Raman/methods ; Gold Colloid/chemistry ; Allergens |
| مستخلص: | Glycinin is one of the most nutritious ingredients in soybean, but it is also an allergen that can cause allergic reactions in humans and animals, and even endanger life in severe cases. Therefore, it is imperative to develop a rapid and ultrasensitive detection method for glycinin. In order to achieve this goal, this experiment combined surface-enhanced Raman spectroscopy (SERS) technology with an economical, simple, fast and easy-to-carry immunochromatographic test strip, and successfully constructed an efficient sandwich immunochromatographic test strip. The immunoprobe of the test strip was covalently coupled by gold nanostars (AuNSs), Raman molecule 4-aminothiophenol (4-PATP) and rabbit polyclonal antibody. In this experiment, a gold nanostar immunochromatographic test strip for detecting glycinin was established. The detection limit of the test strip was 0.23 ng / mL, the recovery rate was 91.5-96.6 %, and the coefficient of variation was 1.61-6.15 %. In addition, the test strip had no cross reaction with whey protein, wheat protein, peanut protein, sesame protein and β-conglycinin, indicating that the detection method had good specificity and the ability to avoid false positive results. Hence, this experiment successfully prepared a rapid and sensitive test strip for glycinin. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antibody; Glycinin; Gold nanostar; LFIA; SERS |
| المشرفين على المادة: | 9007-93-6 (glycinin) 7440-57-5 (Gold) 0 (Antibodies) 0 (Gold Colloid) 0 (Allergens) |
| تواريخ الأحداث: | Date Created: 20230929 Date Completed: 20231108 Latest Revision: 20231108 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.saa.2023.123439 |
| PMID: | 37774588 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3557 |
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| DOI: | 10.1016/j.saa.2023.123439 |