دورية أكاديمية
Comparison of two novel one-tube nested real-time qPCR assays to detect human-infecting Cyclospora spp.
| العنوان: | Comparison of two novel one-tube nested real-time qPCR assays to detect human-infecting Cyclospora spp. |
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| المؤلفون: | Richins T; Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Parasitic Disease Branch , Atlanta, USA.; Oak Ridge Associated Universities , Oak Ridge, Tennessee, USA., Houghton K; University of Tennessee at Knoxville , Knoxville, Tennessee, USA., Barratt J; Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Parasitic Disease Branch , Atlanta, USA.; Oak Ridge Associated Universities , Oak Ridge, Tennessee, USA., H Sapp SG; Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Parasitic Disease Branch , Atlanta, USA., Peterson A; Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Parasitic Disease Branch , Atlanta, USA.; Oak Ridge Associated Universities , Oak Ridge, Tennessee, USA., Qvarnstrom Y; Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Parasitic Disease Branch , Atlanta, USA. |
| المصدر: | Microbiology spectrum [Microbiol Spectr] 2023 Dec 12; Vol. 11 (6), pp. e0138823. Date of Electronic Publication: 2023 Oct 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : ASM Press, 2013- |
| مواضيع طبية MeSH: | Cyclospora*/genetics , Cyclosporiasis*/diagnosis , Cyclosporiasis*/epidemiology, Humans ; DNA, Protozoan ; Disease Outbreaks ; Feces |
| مستخلص: | Importance: Human-infecting Cyclospora spp. cause gastrointestinal distress among healthy individuals contributing to morbidity and putting stress on the economics of countries and companies in the form of produce recalls. Accessible and easy-to-use diagnostic tools available to a wide variety of laboratories would aid in the early detection of possible outbreaks of cyclosporiasis. This, in turn, will assist in the timely traceback investigation to the suspected source of an outbreak by informing the smallest possible recall and protecting consumers from contaminated produce. This manuscript describes two novel detection methods with improved performance for the causative agents of cyclosporiasis when compared to the currently used 18S assay. Competing Interests: The authors declare no conflict of interest. |
| فهرسة مساهمة: | Keywords: Cyclospora cayetanensis; TaqMan; cyclosporiasis; human Cyclospora; nested qPCR; parasitic diagnostics |
| المشرفين على المادة: | 0 (DNA, Protozoan) |
| تواريخ الأحداث: | Date Created: 20231011 Date Completed: 20231216 Latest Revision: 20231216 |
| رمز التحديث: | 20231217 |
| مُعرف محوري في PubMed: | PMC10715049 |
| DOI: | 10.1128/spectrum.01388-23 |
| PMID: | 37819113 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2165-0497 |
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| DOI: | 10.1128/spectrum.01388-23 |