دورية أكاديمية
أعرض في EDS

Myocardial oxidative stress is increased in early reperfusion, but systemic antioxidative therapy does not prevent ischemia-reperfusion arrhythmias in pigs.

التفاصيل البيبلوغرافية
العنوان: Myocardial oxidative stress is increased in early reperfusion, but systemic antioxidative therapy does not prevent ischemia-reperfusion arrhythmias in pigs.
المؤلفون: Haugsten Hansen M; Institute for Experimental Medical Research, Oslo University Hospital and University of Oslo, Oslo, Norway.; KG Jebsen Centre for Cardiac Research, University of Oslo, Oslo, Norway., Sadredini M; Institute for Experimental Medical Research, Oslo University Hospital and University of Oslo, Oslo, Norway.; KG Jebsen Centre for Cardiac Research, University of Oslo, Oslo, Norway., Hasic A; Institute for Experimental Medical Research, Oslo University Hospital and University of Oslo, Oslo, Norway.; KG Jebsen Centre for Cardiac Research, University of Oslo, Oslo, Norway., Eriksen M; Institute for Experimental Medical Research, Oslo University Hospital and University of Oslo, Oslo, Norway.; KG Jebsen Centre for Cardiac Research, University of Oslo, Oslo, Norway., Stokke MK; Institute for Experimental Medical Research, Oslo University Hospital and University of Oslo, Oslo, Norway.; KG Jebsen Centre for Cardiac Research, University of Oslo, Oslo, Norway.; Department of Cardiology, Oslo University Hospital Rikshospitalet, Oslo, Norway.
المصدر: Frontiers in cardiovascular medicine [Front Cardiovasc Med] 2023 Sep 26; Vol. 10, pp. 1223496. Date of Electronic Publication: 2023 Sep 26 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101653388 Publication Model: eCollection Cited Medium: Print ISSN: 2297-055X (Print) Linking ISSN: 2297055X NLM ISO Abbreviation: Front Cardiovasc Med Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media S.A., [2014]-
مستخلص: Background: Arrhythmias in the early phase of reperfusion after myocardial infarction (MI) are common, and can lead to hemodynamic instability or even cardiac arrest. Reactive oxygen species (ROS) are thought to play a key role in the underlying mechanisms, but evidence from large animal models is scarce, and effects of systemic antioxidative treatment remain contentious.
Methods: MI was induced in 7 male and 7 female pigs (Norwegian landrace, 35-40 kg) by clamping of the left anterior descending artery (LAD) during open thorax surgery. Ischemia was maintained for 90 min, before observation for 1 h after reperfusion. Pigs were randomized 1:1 in an operator-blinded fashion to receive either i.v. N-acetylcysteine (NAC) from 70 min of ischemia and onwards, or 0.9% NaCl as a control. Blood samples and tissue biopsies were collected at baseline, 60 min of ischemia, and 5 and 60 min of reperfusion. ECG and invasive blood pressure were monitored throughout.
Results: The protocol was completed in 11 pigs. Oxidative stress, as indicated by immunoblotting for Malondialdehyde in myocardial biopsies, was increased at 5 min of reperfusion compared to baseline, but not at 60 min of reperfusion, and not reduced with NAC. We found no significant differences in circulating biomarkers of myocardial necrosis, nor in the incidence of idioventricular rhythm (IVR), non-sustained ventricular tachycardia (NSVT), ventricular tachycardia (VT) or ventricular fibrillation (VF) between NAC-treated and control pigs during reperfusion.
Conclusion: Myocardial oxidation was increased early after reperfusion in a porcine model of MI, but systemic antioxidative treatment did not protect against reperfusion arrhythmias.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(© 2023 Haugsten Hansen, Sadredini, Hasic, Eriksen and Stokke.)
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فهرسة مساهمة: Keywords: antioxidant; arrhythmias; ischemia-reperfusion; large animal model; myocardial infarction; n-acetylcysteine; pig; translational research
تواريخ الأحداث: Date Created: 20231012 Latest Revision: 20231030
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC10562584
DOI: 10.3389/fcvm.2023.1223496
PMID: 37823177
قاعدة البيانات: MEDLINE
الوصف
تدمد:2297-055X
DOI:10.3389/fcvm.2023.1223496