دورية أكاديمية
Aldo-keto Reductase 1B10 Restrains Cell Migration, Invasion, and Adhesion of Gastric Cancer via Regulating Integrin Subunit Alpha 5.
| العنوان: | Aldo-keto Reductase 1B10 Restrains Cell Migration, Invasion, and Adhesion of Gastric Cancer via Regulating Integrin Subunit Alpha 5. |
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| المؤلفون: | Yao H; Department of Hepatobiliary - Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China; Division of General Surgery, Department of Gastrointestinal and Pancreatic Surgery, Cancer Center, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College), Hangzhou, Zhejiang, China., Hu J; Division of General Surgery, Department of Gastrointestinal and Pancreatic Surgery, Cancer Center, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College), Hangzhou, Zhejiang, China., Shao Y; Department of Pharmacy, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College), Hangzhou, Zhejiang, China., Shah Q; Division of General Surgery, Department of Gastrointestinal and Pancreatic Surgery, Cancer Center, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College), Hangzhou, Zhejiang, China., Zheng S; Department of Hepatobiliary - Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. |
| المصدر: | The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology [Turk J Gastroenterol] 2023 Dec; Vol. 34 (12), pp. 1197-1205. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Aves Country of Publication: Turkey NLM ID: 9515841 Publication Model: Print Cited Medium: Internet ISSN: 2148-5607 (Electronic) Linking ISSN: 13004948 NLM ISO Abbreviation: Turk J Gastroenterol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2014- : İstanbul,Turkey : Aves Original Publication: Ankara : Turkish Society of Gastroenterology, 1995- |
| مواضيع طبية MeSH: | Aldo-Keto Reductases*/genetics , Integrins* , Stomach Neoplasms*/pathology, Humans ; Aldehyde Reductase/genetics ; Aldehyde Reductase/metabolism ; Cell Line, Tumor ; Cell Movement/genetics ; Cell Proliferation/genetics |
| مستخلص: | Background/aims: Gastric cancer is a prevalent malignancy with unfavorable prognosis partially resulting from its high metastasis rate. Clarifying the molecular mechanism of gastric cancer occurrence and progression for improvement of therapeutic efficacy and prognosis is needed. The study tended to delineate the role and regulatory mechanism of aldo-keto reductase 1B10 (AKR1B10) in gastric cancer progression. Materials and Methods: The relationship of AKR1B10 expression with survival rate in gastric cancer was analyzed through Kaplan-Meier analysis. The mRNA levels of AKR1B10 and integrin subunit alpha 5 (ITGA5) in gastric cancer tissues and cell lines were measured by real-time quantitative polymerase chain reaction. Protein levels of AKR1B10 and integrin subunit alpha 5 were assayed via western blot. The molecular relationship between AKR1B10 and ITGA5 was analyzed by co-immunoprecipitation assay. Cell viability was assayed through Cell Counting Kit-8, invasion and migration of tumor cells was assessed through wound healing and transwell assays. Transwell assay was utilized to detect invasion. The adhesion of gastric cancer cells was detected using cell adhesion assays. Results: The results unveiled that integrin subunit alpha 5 was upregulated, while AKR1B10 was downregulated in gastric cancer tissues and cells. Overexpressing AKR1B10 hindered gastric cancer cell proliferation, migration, invasion and adhesion. It was striking that we certified the inhibitory effect of AKR1B10 on integrin subunit alpha 5 expression and their (AKR1B10 and ITGA5)) negative relationship via bioinformatics method, real-time quantitative polymerase chain reaction, and co-immunoprecipitation assays. Via rescue experiments, it was concluded that AKR1B10 served as tumor suppressor potentially by ITGA5 expression in gastric cancer. Conclusion: Our results indicated that AKR1B10 inhibited migration, invasion, and adhesion of gastric cancer cells via modulation of ITGA5. |
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| المشرفين على المادة: | EC 1.1.1.21 (Aldehyde Reductase) EC 1.1.1.- (Aldo-Keto Reductases) 0 (Integrins) EC 1.1.1.- (AKR1B10 protein, human) 0 (ITGA5 protein, human) |
| تواريخ الأحداث: | Date Created: 20231012 Date Completed: 20231222 Latest Revision: 20240328 |
| رمز التحديث: | 20240329 |
| مُعرف محوري في PubMed: | PMC10765221 |
| DOI: | 10.5152/tjg.2023.22555 |
| PMID: | 37823316 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2148-5607 |
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| DOI: | 10.5152/tjg.2023.22555 |