دورية أكاديمية
أعرض في EDS

A double-negative feedback loop between NtrBC and a small RNA rewires nitrogen metabolism in legume symbionts.

التفاصيل البيبلوغرافية
العنوان: A double-negative feedback loop between NtrBC and a small RNA rewires nitrogen metabolism in legume symbionts.
المؤلفون: García-Tomsig NI; Structure, Dynamics and Function of Rhizobacterial Genomes (RhizoRNA Lab), Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Granada, Spain., García-Rodriguez FM; Structure, Dynamics and Function of Rhizobacterial Genomes (RhizoRNA Lab), Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Granada, Spain., Guedes-García SK; Structure, Dynamics and Function of Rhizobacterial Genomes (RhizoRNA Lab), Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Granada, Spain., Millán V; Structure, Dynamics and Function of Rhizobacterial Genomes (RhizoRNA Lab), Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Granada, Spain., Becker A; Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Marburg, Germany., Robledo M; Structure, Dynamics and Function of Rhizobacterial Genomes (RhizoRNA Lab), Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Granada, Spain., Jiménez-Zurdo JI; Structure, Dynamics and Function of Rhizobacterial Genomes (RhizoRNA Lab), Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Granada, Spain.
المصدر: MBio [mBio] 2023 Dec 19; Vol. 14 (6), pp. e0200323. Date of Electronic Publication: 2023 Oct 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101519231 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2150-7511 (Electronic) NLM ISO Abbreviation: mBio Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, D.C. : American Society for Microbiology
مستخلص: Importance: Root nodule endosymbioses between diazotrophic rhizobia and legumes provide the largest input of combined N to the biosphere, thus representing an alternative to harmful chemical fertilizers for sustainable crop production. Rhizobia have evolved intricate strategies to coordinate N assimilation for their own benefit with N 2 fixation to sustain plant growth. The rhizobial N status is transduced by the NtrBC two-component system, the seemingly ubiquitous form of N signal transduction in Proteobacteria. Here, we show that the regulatory sRNA NfeR1 (nodule formation efficiency RNA) of the alfalfa symbiont Sinorhizobium meliloti is transcribed from a complex promoter repressed by NtrC in a N-dependent manner and feedback silences ntrBC by complementary base-pairing. These findings unveil a more prominent role of NtrC as a transcriptional repressor than hitherto anticipated and a novel RNA-based mechanism for NtrBC regulation. The NtrBC-NfeR1 double-negative feedback loop accurately rewires symbiotic S. meliloti N metabolism and is likely conserved in α-rhizobia.
Competing Interests: The authors declare no conflict of interest.
معلومات مُعتمدة: PID2020-114782GB-I00 Ministerio de Ciencia e Innovación (MCIN); P20_00185 Junta de Andalucía (Andalusian Board); BE 2121/9-1 German Research Foundation; FPU16/01275 Ministerio de Universidades (MU); FPU21/05195 Ministerio de Universidades (MU)
فهرسة مساهمة: Keywords: Sinorhizobium meliloti; alpha-proteobacteria; nitrogen fixation; rhizobia; riboregulation; two-component regulatory systems
تواريخ الأحداث: Date Created: 20231018 Latest Revision: 20240727
رمز التحديث: 20240727
مُعرف محوري في PubMed: PMC10746234
DOI: 10.1128/mbio.02003-23
PMID: 37850753
قاعدة البيانات: MEDLINE
الوصف
تدمد:2150-7511
DOI:10.1128/mbio.02003-23