دورية أكاديمية
Protocol for cytokine and uterine immune cell characterization in a mouse model of LPS-induced preterm birth.
| العنوان: | Protocol for cytokine and uterine immune cell characterization in a mouse model of LPS-induced preterm birth. |
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| المؤلفون: | Wayland JL; Immunology Graduate Program, University of Cincinnati College of Medicine, Cincinnati, OH 45220, USA; Medical Scientist Training Program, University of Cincinnati College of Medicine, Cincinnati, OH 45220, USA. Electronic address: jennifer.wayland@cchmc.org., Stemen EL; Division of Immunobiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA., Doll JR; Division of Immunobiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA., Divanovic S; Immunology Graduate Program, University of Cincinnati College of Medicine, Cincinnati, OH 45220, USA; Medical Scientist Training Program, University of Cincinnati College of Medicine, Cincinnati, OH 45220, USA; Division of Immunobiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45220, USA; Center for Inflammation and Tolerance, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA. Electronic address: senad.divanovic@cchmc.org. |
| المصدر: | STAR protocols [STAR Protoc] 2023 Dec 15; Vol. 4 (4), pp. 102643. Date of Electronic Publication: 2023 Oct 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Cytokines*/adverse effects , Premature Birth*/chemically induced, Infant, Newborn ; Female ; Humans ; Animals ; Mice ; Lipopolysaccharides/adverse effects ; Uterus ; Inflammation/chemically induced |
| مستخلص: | Inflammation-driven preterm birth (PTB) is modeled in mice using lipopolysaccharide (LPS) challenge. Here, we present a protocol for cytokine and uterine immune cell characterization in a mouse model of LPS-induced PTB. We describe steps for LPS challenge, in vivo cytokine capture assay, and isolation of uterine immune cells for flow cytometry. These techniques allow examination of systemic inflammation in vivo and immune cell characterization at the maternal-fetal interface, facilitating exploration of inflammatory dynamics in mouse models of PTB susceptibility. For complete details on the use and execution of this protocol, please refer to Doll et al. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cell Isolation; Flow Cytometry; Immunology; Model Organisms |
| المشرفين على المادة: | 0 (Cytokines) 0 (Lipopolysaccharides) |
| تواريخ الأحداث: | Date Created: 20231020 Date Completed: 20240311 Latest Revision: 20240311 |
| رمز التحديث: | 20240311 |
| مُعرف محوري في PubMed: | PMC10594632 |
| DOI: | 10.1016/j.xpro.2023.102643 |
| PMID: | 37858473 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2023.102643 |