دورية أكاديمية
Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas.
| العنوان: | Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas. |
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| المؤلفون: | Steiger L; Clinic of Endocrinology, Diabetes and Metabolism, University Hospital Basel, Basel, Switzerland; Department of Biomedicine, University of Basel, Basel, Switzerland. Electronic address: laura.steiger@unibas.ch., Baumann Z; Tumor Heterogeneity, Metastasis and Resistance, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland; Department of Surgery, University Hospital Basel, Basel, Switzerland., Keller L; Clinic of Endocrinology, Diabetes and Metabolism, University Hospital Basel, Basel, Switzerland; Department of Biomedicine, University of Basel, Basel, Switzerland., Böni-Schnetzler M; Clinic of Endocrinology, Diabetes and Metabolism, University Hospital Basel, Basel, Switzerland; Department of Biomedicine, University of Basel, Basel, Switzerland., Donath MY; Clinic of Endocrinology, Diabetes and Metabolism, University Hospital Basel, Basel, Switzerland; Department of Biomedicine, University of Basel, Basel, Switzerland., Meier DT; Clinic of Endocrinology, Diabetes and Metabolism, University Hospital Basel, Basel, Switzerland; Department of Biomedicine, University of Basel, Basel, Switzerland. Electronic address: daniel.zeman@unibas.ch. |
| المصدر: | STAR protocols [STAR Protoc] 2023 Dec 15; Vol. 4 (4), pp. 102664. Date of Electronic Publication: 2023 Oct 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Islets of Langerhans* , Pancreas, Exocrine*, Mice ; Animals ; Flow Cytometry |
| مستخلص: | Diabetes mellitus is a disease of the hormone-secreting endocrine pancreas. However, increasing evidence suggests that the exocrine pancreas is also involved in the pathogenesis of diabetes. In this protocol, we describe how to harvest both isolated islets and exocrine tissue from one mouse pancreas, followed by a detailed explanation of how to isolate and analyze immune cells using full-spectrum flow cytometry. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cell Isolation; Cell Separation/Fractionation; Flow Cytometry |
| تواريخ الأحداث: | Date Created: 20231027 Date Completed: 20240311 Latest Revision: 20240311 |
| رمز التحديث: | 20240311 |
| مُعرف محوري في PubMed: | PMC10641308 |
| DOI: | 10.1016/j.xpro.2023.102664 |
| PMID: | 37889759 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2023.102664 |