دورية أكاديمية
أعرض في EDS

Chromatinization modulates topoisomerase II processivity.

التفاصيل البيبلوغرافية
العنوان: Chromatinization modulates topoisomerase II processivity.
المؤلفون: Lee J; Physics Department & LASSP, Cornell University, Ithaca, NY, 14853, USA., Wu M; Physics Department & LASSP, Cornell University, Ithaca, NY, 14853, USA.; Howard Hughes Medical Institute, Cornell University, Ithaca, NY, 14853, USA., Inman JT; Physics Department & LASSP, Cornell University, Ithaca, NY, 14853, USA.; Howard Hughes Medical Institute, Cornell University, Ithaca, NY, 14853, USA., Singh G; Biophysics Program, Cornell University, Ithaca, NY, 14853, USA., Park SH; Biophysics Program, Cornell University, Ithaca, NY, 14853, USA., Lee JH; Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA., Fulbright RM; Physics Department & LASSP, Cornell University, Ithaca, NY, 14853, USA., Hong Y; Department of Electrical and Computer Engineering, Cornell University, Ithaca, NY, 14853, USA., Jeong J; Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA., Berger JM; Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA., Wang MD; Physics Department & LASSP, Cornell University, Ithaca, NY, 14853, USA. mwang@physics.cornell.edu.; Howard Hughes Medical Institute, Cornell University, Ithaca, NY, 14853, USA. mwang@physics.cornell.edu.
المصدر: Nature communications [Nat Commun] 2023 Oct 27; Vol. 14 (1), pp. 6844. Date of Electronic Publication: 2023 Oct 27.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: DNA Topoisomerases, Type II*/metabolism , DNA*, Chromatin ; DNA Topoisomerases, Type I/metabolism ; Eukaryotic Cells/metabolism
مستخلص: Type IIA topoisomerases are essential DNA processing enzymes that must robustly and reliably relax DNA torsional stress. While cellular processes constantly create varying torsional stress, how this variation impacts type IIA topoisomerase function remains obscure. Using multiple single-molecule approaches, we examined the torsional dependence of eukaryotic topoisomerase II (topo II) activity on naked DNA and chromatin. We observed that topo II is ~50-fold more processive on buckled DNA than previously estimated. We further discovered that topo II relaxes supercoiled DNA prior to plectoneme formation, but with processivity reduced by ~100-fold. This relaxation decreases with diminishing torsion, consistent with topo II capturing transient DNA loops. Topo II retains high processivity on buckled chromatin (~10,000 turns) and becomes highly processive even on chromatin under low torsional stress (~1000 turns), consistent with chromatin's predisposition to readily form DNA crossings. This work establishes that chromatin is a major stimulant of topo II function.
(© 2023. The Author(s).)
التعليقات: Update of: bioRxiv. 2023 Oct 04;:. (PMID: 37873421)
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معلومات مُعتمدة: R01 CA077373 United States CA NCI NIH HHS; R01 GM136894 United States GM NIGMS NIH HHS; R35 CA263778 United States CA NCI NIH HHS; T32 GM008267 United States GM NIGMS NIH HHS
المشرفين على المادة: EC 5.99.1.3 (DNA Topoisomerases, Type II)
9007-49-2 (DNA)
0 (Chromatin)
EC 5.99.1.2 (DNA Topoisomerases, Type I)
تواريخ الأحداث: Date Created: 20231027 Date Completed: 20231030 Latest Revision: 20240210
رمز التحديث: 20240210
مُعرف محوري في PubMed: PMC10611788
DOI: 10.1038/s41467-023-42600-z
PMID: 37891161
قاعدة البيانات: MEDLINE
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