دورية أكاديمية
أعرض في EDS

Endothelialization of Whey Protein Isolate-Based Scaffolds for Tissue Regeneration.

التفاصيل البيبلوغرافية
العنوان: Endothelialization of Whey Protein Isolate-Based Scaffolds for Tissue Regeneration.
المؤلفون: Genç H; Section of Experimental Oncology und Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, Department of Otorhinolaryngology, Head and Neck Surgery, Universitätsklinikum Erlangen, University of Erlangen-Nürnberg, 91054 Erlangen, Germany., Friedrich B; Section of Experimental Oncology und Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, Department of Otorhinolaryngology, Head and Neck Surgery, Universitätsklinikum Erlangen, University of Erlangen-Nürnberg, 91054 Erlangen, Germany., Alexiou C; Section of Experimental Oncology und Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, Department of Otorhinolaryngology, Head and Neck Surgery, Universitätsklinikum Erlangen, University of Erlangen-Nürnberg, 91054 Erlangen, Germany., Pietryga K; Silesian Park of Medical Technology Kardio-Med Silesia, 41-800 Zabrze, Poland., Cicha I; Section of Experimental Oncology und Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, Department of Otorhinolaryngology, Head and Neck Surgery, Universitätsklinikum Erlangen, University of Erlangen-Nürnberg, 91054 Erlangen, Germany., Douglas TEL; School of Engineering, Lancaster University, Lancaster LA1 4YX, UK.
المصدر: Molecules (Basel, Switzerland) [Molecules] 2023 Oct 12; Vol. 28 (20). Date of Electronic Publication: 2023 Oct 12.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c1995-
مواضيع طبية MeSH: Osteogenesis* , Endothelial Cells*, Humans ; Whey Proteins/pharmacology ; Hydrogels/pharmacology ; Cell Differentiation ; Tissue Scaffolds
مستخلص: Background: Whey protein isolate (WPI) is a by-product from the dairy industry, whose main component is β-lactoglobulin. Upon heating, WPI forms a hydrogel which can both support controlled drug delivery and enhance the proliferation and osteogenic differentiation of bone-forming cells. This study makes a novel contribution by evaluating the ability of WPI hydrogels to support the growth of endothelial cells, which are essential for vascularization, which in turn is a pre-requisite for bone regeneration.
Methods: In this study, the proliferation and antioxidant levels in human umbilical vascular endothelial cells (HUVECs) cultured with WPI supplementation were evaluated using real-time cell analysis and flow cytometry. Further, the attachment and growth of HUVECs seeded on WPI-based hydrogels with different concentrations of WPI (15%, 20%, 30%, 40%) were investigated.
Results: Supplementation with WPI did not affect the viability or proliferation of HUVECs monitored with real-time cell analysis. At the highest used concentration of WPI (500 µg/mL), a slight induction of ROS production in HUVECs was detected as compared with control samples, but it was not accompanied by alterations in cellular thiol levels. Regarding WPI-based hydrogels, HUVEC adhered and spread on all samples, showing good metabolic activity. Notably, cell number was highest on samples containing 20% and 30% WPI.
Conclusions: The demonstration of the good compatibility of WPI hydrogels with endothelial cells in these experiments is an important step towards promoting the vascularization of hydrogels upon implantation in vivo, which is expected to improve implant outcomes in the future.
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فهرسة مساهمة: Keywords: 3D cell seeding; endothelial cell compatibility; hydrogels; thiol levels; tubular scaffolds; whey protein
المشرفين على المادة: 0 (Whey Proteins)
0 (Hydrogels)
تواريخ الأحداث: Date Created: 20231028 Date Completed: 20231030 Latest Revision: 20231031
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10609092
DOI: 10.3390/molecules28207052
PMID: 37894531
قاعدة البيانات: MEDLINE
الوصف
تدمد:1420-3049
DOI:10.3390/molecules28207052