First in human measurements of abscess cavity optical properties and methylene blue uptake prior to photodynamic therapy by in vivo diffuse reflectance spectroscopy.
| العنوان: | First in human measurements of abscess cavity optical properties and methylene blue uptake prior to photodynamic therapy by in vivo diffuse reflectance spectroscopy. |
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| المؤلفون: | Hannan MN, Sharma AK, Baran TM |
| المصدر: | MedRxiv : the preprint server for health sciences [medRxiv] 2023 Oct 16. Date of Electronic Publication: 2023 Oct 16. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101767986 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: medRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | Significance: Efficacious photodynamic therapy (PDT) of abscess cavities requires personalized treatment planning. This relies on knowledge of abscess wall optical properties, which we report for the first time in human subjects. Aim: The objective was to extract optical properties and photosensitizer concentration from spatially-resolved diffuse reflectance measurements of abscess cavities prior to methylene blue (MB) PDT, as part of a Phase 1 clinical trial. Approach: Diffuse reflectance spectra were collected at the abscess wall of 13 human subjects using a custom fiber-optic probe and optical spectroscopy system, before and after MB administration. A Monte Carlo lookup table was used to extract optical properties. Results: Pre-MB abscess wall absorption coefficients at 665 nm were 0.15±0.1 cm -1 (0.03-0.36 cm -1 ) and 10.74±15.81 cm -1 (0.08-49.3 cm -1 ) post-MB. Reduced scattering coefficients at 665 nm were 8.45±2.37 cm -1 (4.8-13.2 cm -1 ) and 5.6±2.26 cm -1 (1.6-9.9 cm -1 ) for pre-MB and post-MB, respectively. Oxygen saturations were found to be 58.83±35.78% (5.6-100%) pre-MB and 36.29±25.1% (0.0001-76.4%) post-MB. Determined MB concentrations were 71.83±108.22 µM (0-311 µM). Conclusions: We observed substantial inter-subject variation in both native wall optical properties and methylene blue uptake. This underscores the importance of making these measurements for patient-specific treatment planning. |
| التعليقات: | Update in: J Biomed Opt. 2024 Feb;29(2):027002. (PMID: 38414658) |
| معلومات مُعتمدة: | R01 EB029921 United States EB NIBIB NIH HHS |
| تواريخ الأحداث: | Date Created: 20231031 Latest Revision: 20240311 |
| رمز التحديث: | 20240311 |
| مُعرف محوري في PubMed: | PMC10615020 |
| DOI: | 10.1101/2023.10.16.23297088 |
| PMID: | 37905076 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.1101/2023.10.16.23297088 |
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