دورية أكاديمية
Leucine 127 of Cucurbit Chlorotic Yellows Virus P22 Is Crucial for Its RNA Silencing Suppression Activity and Pathogenicity.
| العنوان: | Leucine 127 of Cucurbit Chlorotic Yellows Virus P22 Is Crucial for Its RNA Silencing Suppression Activity and Pathogenicity. |
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| المؤلفون: | Kang YC; Department of Medical Laboratory Science and Biotechnology, Asia University, Wufeng, Taichung 41354, Taiwan.; Department of Plant Pathology, National Chung Hsing University, Taichung 40227, Taiwan., Yeh SD; Department of Plant Pathology, National Chung Hsing University, Taichung 40227, Taiwan.; Advanced Plant Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan., Chen TC; Department of Medical Laboratory Science and Biotechnology, Asia University, Wufeng, Taichung 41354, Taiwan. |
| المصدر: | Phytopathology [Phytopathology] 2024 Apr; Vol. 114 (4), pp. 813-822. Date of Electronic Publication: 2024 Apr 10. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Phytopathological Society] Country of Publication: United States NLM ID: 9427222 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0031-949X (Print) Linking ISSN: 0031949X NLM ISO Abbreviation: Phytopathology Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [St. Paul, Minn., etc., American Phytopathological Society] |
| مواضيع طبية MeSH: | Plant Diseases*/virology , Crinivirus*/genetics , Crinivirus*/pathogenicity , Chenopodium quinoa*/virology , Plant Leaves*/virology , Viral Proteins*/genetics , Viral Proteins*/metabolism , RNA Interference* , Leucine*/metabolism , Potyvirus*, Virulence ; Cucurbita/virology |
| مستخلص: | Plant viruses produce particular suppressors to antagonize the host defense response of RNA silencing to establish infection. Cucurbit chlorotic yellows virus (CCYV), a member of the genus Crinivirus of the family Closteroviridae , severely damages the production of economically essential cucurbits worldwide. Here, we used the attenuated zucchini yellow mosaic virus (ZYMV) vector ZAC to express individual coding sequences, including CP, CPm, P25, and P22, of a Taiwan CCYV isolate (CCYV-TW) to identify their possible roles as pathogenicity determinants. ZAC is an HC-Pro function mutant that lacks the ability of local lesion induction on Chenopodium quinoa leaves and induces mild mottling followed by recovery on its natural host zucchini squash plants. Only the recombinant expressing CCYV-TW P22 complemented the effect of ZAC HC-Pro dysfunction, causing more severe symptoms on zucchini squash plants and restoring lesion formation on C. quinoa leaves, with lesions forming faster than those generated by the wild-type ZYMV. This suggests that CCYV-TW P22 is a virulence enhancer. Sequence analysis of criniviral P22s revealed the presence of four conserved leucine residues (L Competing Interests: The author(s) declare no conflict of interest. |
| فهرسة مساهمة: | Keywords: RNA silencing suppressor; crinivirus; cucurbit chlorotic yellows virus (CCYV); pathogenicity |
| المشرفين على المادة: | 0 (Viral Proteins) GMW67QNF9C (Leucine) |
| SCR Organism: | Zucchini yellow mosaic virus |
| تواريخ الأحداث: | Date Created: 20231101 Date Completed: 20240430 Latest Revision: 20240430 |
| رمز التحديث: | 20240430 |
| DOI: | 10.1094/PHYTO-07-23-0227-R |
| PMID: | 37913633 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0031-949X |
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| DOI: | 10.1094/PHYTO-07-23-0227-R |