دورية أكاديمية
Usf2 Deficiency Promotes Autophagy to Alleviate Cerebral Ischemia-Reperfusion Injury Through Suppressing YTHDF1-m6A-Mediated Cdc25A Translation.
العنوان: | Usf2 Deficiency Promotes Autophagy to Alleviate Cerebral Ischemia-Reperfusion Injury Through Suppressing YTHDF1-m6A-Mediated Cdc25A Translation. |
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المؤلفون: | Liu C; Department of Vascular Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, 710061, China., Gao Q; Department of Anesthesia Operation, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, 710061, China., Dong J; Department of Vascular Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, 710061, China., Cai H; Department of Vascular Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, 710061, China. xenrang27267@163.com. |
المصدر: | Molecular neurobiology [Mol Neurobiol] 2024 May; Vol. 61 (5), pp. 2556-2568. Date of Electronic Publication: 2023 Nov 02. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 8900963 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-1182 (Electronic) Linking ISSN: 08937648 NLM ISO Abbreviation: Mol Neurobiol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Clifton, NJ : Humana Press, c1987- |
مواضيع طبية MeSH: | Adenosine*/analogs & derivatives , Adenosine*/metabolism , Autophagy*/physiology , Autophagy*/genetics , Mice, Inbred C57BL* , Neurons*/metabolism , Neurons*/pathology , Reperfusion Injury*/metabolism , Reperfusion Injury*/pathology , Reperfusion Injury*/genetics , RNA-Binding Proteins*/metabolism , RNA-Binding Proteins*/genetics , Upstream Stimulatory Factors*/metabolism , Upstream Stimulatory Factors*/genetics, Animals ; Male ; Mice ; Apoptosis ; Brain Ischemia/metabolism ; Brain Ischemia/pathology ; Cell Line ; Cell Survival ; Infarction, Middle Cerebral Artery/pathology ; Infarction, Middle Cerebral Artery/metabolism ; Protein Biosynthesis |
مستخلص: | Autophagy has been involved in protection of ischemia/reperfusion (I/R)-induced injury in many tissues including the brain. The upstream stimulatory factor 2 (Usf2) was proposed as a regulator in aging and degenerative brain diseases; however, the its role in autophagy during cerebral I/R injury remains unclear. Here, the middle cerebral artery occlusion (MCAO) operation was applied to establish an I/R mouse model. We showed that Usf2 was significantly upregulated in I/R-injured brain, accompanied by decreased levels of autophagy. Then, oxygen-glucose deprivation/recovery (OGD/R) treatment was used to establish a cellular I/R model in HT22 neurons, and lentiviral interference vector against Usf2 (LV-sh-Usf2) was used to infect the neurons. Our results showed that Usf2 was significantly upregulated in OGD/R-treated HT22 neurons that displayed an increased level in cell apoptosis and decreased levels in cell viability and autophagy, and interference of Usf2 largely rescued the effects of OGD/R on cell viability, apoptosis, and autophagy, suggesting an important role of Usf2 in neuron autophagy. In the mechanism exploration, we found that, as a transcription factor, Usf2 bound to the promoter of YTHDF1, a famous reader of N6-Methyladenosine (m6A), also induced by OGD/R, and promoted its transcription. Overexpression of YTHDF1 was able to reverse the improvement of Usf2 interference on viability and autophagy of HT22 neurons. Moreover, YTHDF1 suppressed autophagy to induce HT22 cell apoptosis through increasing m6A-mediated stability of Cdc25A, a newly identified autophagy inhibitor. Finally, we demonstrated that interference of Usf2 markedly improved autophagy and alleviated I/R-induced injury in MCAO mice. (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.) |
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فهرسة مساهمة: | Keywords: Autophagy; Cdc25A; Cerebral ischemia-reperfusion injury; The m6A reader YTHDF1; Usf2 |
المشرفين على المادة: | K72T3FS567 (Adenosine) CLE6G00625 (N-methyladenosine) 0 (RNA-Binding Proteins) 0 (Upstream Stimulatory Factors) 0 (Ythdf1 protein, mouse) EC 3.1.3.48 (Cdc25a protein, mouse) 0 (Usf2 protein, mouse) |
تواريخ الأحداث: | Date Created: 20231102 Date Completed: 20240424 Latest Revision: 20240506 |
رمز التحديث: | 20240507 |
DOI: | 10.1007/s12035-023-03735-8 |
PMID: | 37914905 |
قاعدة البيانات: | MEDLINE |
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