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GATA transcription factors drive initial Xist upregulation after fertilization through direct activation of long-range enhancers.

التفاصيل البيبلوغرافية
العنوان: GATA transcription factors drive initial Xist upregulation after fertilization through direct activation of long-range enhancers.
المؤلفون: Ravid Lustig L; Systems Epigenetics, Otto Warburg Laboratories, Max Planck Institute for Molecular Genetics, Berlin, Germany., Sampath Kumar A; Department of Genome Regulation, Max Planck Institute for Molecular Genetics, Berlin, Germany.; Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany., Schwämmle T; Systems Epigenetics, Otto Warburg Laboratories, Max Planck Institute for Molecular Genetics, Berlin, Germany., Dunkel I; Systems Epigenetics, Otto Warburg Laboratories, Max Planck Institute for Molecular Genetics, Berlin, Germany., Noviello G; Systems Epigenetics, Otto Warburg Laboratories, Max Planck Institute for Molecular Genetics, Berlin, Germany., Limberg E; Systems Epigenetics, Otto Warburg Laboratories, Max Planck Institute for Molecular Genetics, Berlin, Germany., Weigert R; Department of Genome Regulation, Max Planck Institute for Molecular Genetics, Berlin, Germany.; Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany., Pacini G; Systems Epigenetics, Otto Warburg Laboratories, Max Planck Institute for Molecular Genetics, Berlin, Germany., Buschow R; Microscopy and Cryo-Electron Microscopy, Max Planck Institute for Molecular Genetics, Berlin, Germany., Ghauri A; Systems Epigenetics, Otto Warburg Laboratories, Max Planck Institute for Molecular Genetics, Berlin, Germany., Stötzel M; Department of Genome Regulation, Max Planck Institute for Molecular Genetics, Berlin, Germany., Wittler L; Transgenic Unit, Max Planck Institute for Molecular Genetics, Berlin, Germany., Meissner A; Department of Genome Regulation, Max Planck Institute for Molecular Genetics, Berlin, Germany., Schulz EG; Systems Epigenetics, Otto Warburg Laboratories, Max Planck Institute for Molecular Genetics, Berlin, Germany. edda.schulz@molgen.mpg.de.
المصدر: Nature cell biology [Nat Cell Biol] 2023 Nov; Vol. 25 (11), pp. 1704-1715. Date of Electronic Publication: 2023 Nov 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Macmillan Magazines Ltd Country of Publication: England NLM ID: 100890575 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4679 (Electronic) Linking ISSN: 14657392 NLM ISO Abbreviation: Nat Cell Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Macmillan Magazines Ltd., [1999-
مواضيع طبية MeSH: GATA Transcription Factors*/genetics , RNA, Long Noncoding*/genetics, Animals ; Female ; Mice ; Fertilization/genetics ; Mammals ; Up-Regulation ; X Chromosome ; X Chromosome Inactivation/genetics
مستخلص: X-chromosome inactivation (XCI) balances gene expression between the sexes in female mammals. Shortly after fertilization, upregulation of Xist RNA from one X chromosome initiates XCI, leading to chromosome-wide gene silencing. XCI is maintained in all cell types, except the germ line and the pluripotent state where XCI is reversed. The mechanisms triggering Xist upregulation have remained elusive. Here we identify GATA transcription factors as potent activators of Xist. Through a pooled CRISPR activation screen in murine embryonic stem cells, we demonstrate that GATA1, as well as other GATA transcription factors can drive ectopic Xist expression. Moreover, we describe GATA-responsive regulatory elements in the Xist locus bound by different GATA factors. Finally, we show that GATA factors are essential for XCI induction in mouse preimplantation embryos. Deletion of GATA1/4/6 or GATA-responsive Xist enhancers in mouse zygotes effectively prevents Xist upregulation. We propose that the activity or complete absence of various GATA family members controls initial Xist upregulation, XCI maintenance in extra-embryonic lineages and XCI reversal in the epiblast.
(© 2023. The Author(s).)
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معلومات مُعتمدة: K05 DA000064 United States DA NIDA NIH HHS
المشرفين على المادة: 0 (GATA Transcription Factors)
0 (RNA, Long Noncoding)
0 (XIST non-coding RNA)
تواريخ الأحداث: Date Created: 20231106 Date Completed: 20231116 Latest Revision: 20240210
رمز التحديث: 20240210
مُعرف محوري في PubMed: PMC10635832
DOI: 10.1038/s41556-023-01266-x
PMID: 37932452
قاعدة البيانات: MEDLINE
الوصف
تدمد:1476-4679
DOI:10.1038/s41556-023-01266-x