دورية أكاديمية
The branched chain amino acids (BCAAs) modulate the development of the intra-cellular stages of Trypanosoma cruzi.
| العنوان: | The branched chain amino acids (BCAAs) modulate the development of the intra-cellular stages of Trypanosoma cruzi. |
|---|---|
| المؤلفون: | Rapado LN; LaBTryps, Instituto de Ciencias Biomedicas II, Universidade de São Paulo, Av. Lineu Prestes 1374, Cidade Universitária Butanta, São Paulo, CEP 05508-900, Brazil., Nascimento JF; LaBTryps, Instituto de Ciencias Biomedicas II, Universidade de São Paulo, Av. Lineu Prestes 1374, Cidade Universitária Butanta, São Paulo, CEP 05508-900, Brazil., Manchola NC; LaBTryps, Instituto de Ciencias Biomedicas II, Universidade de São Paulo, Av. Lineu Prestes 1374, Cidade Universitária Butanta, São Paulo, CEP 05508-900, Brazil., Damasceno FS; LaBTryps, Instituto de Ciencias Biomedicas II, Universidade de São Paulo, Av. Lineu Prestes 1374, Cidade Universitária Butanta, São Paulo, CEP 05508-900, Brazil., Achjian RW; LaBTryps, Instituto de Ciencias Biomedicas II, Universidade de São Paulo, Av. Lineu Prestes 1374, Cidade Universitária Butanta, São Paulo, CEP 05508-900, Brazil., Silber AM; LaBTryps, Instituto de Ciencias Biomedicas II, Universidade de São Paulo, Av. Lineu Prestes 1374, Cidade Universitária Butanta, São Paulo, CEP 05508-900, Brazil. Electronic address: asilber@usp.br. |
| المصدر: | Experimental parasitology [Exp Parasitol] 2023 Dec; Vol. 255, pp. 108642. Date of Electronic Publication: 2023 Nov 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0370713 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2449 (Electronic) Linking ISSN: 00144894 NLM ISO Abbreviation: Exp Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando, FL : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Trypanosoma cruzi* , Chagas Disease*/parasitology, Animals ; Amino Acids, Branched-Chain/metabolism ; Leucine ; Protozoan Proteins/metabolism ; Life Cycle Stages |
| مستخلص: | The life cycle of Trypanosoma cruzi, the etiological agent of Chagas disease, involves different forms of the parasite, which alternates between insect and vertebrate hosts. One critical process in the parasite's life cycle is metacyclogenesis, in which the replicative non-infective forms present in the insect midgut differentiate into non-dividing vertebrate-infective forms. It is known that proline (Pro) is important for this process and that leucine (Leu) and isoleucine (Ile) can act as inhibitors of metacyclogenesis. In this study, we investigated further the role of branched-chain amino acids (BCAAs) as negative modulators of parasite differentiation and infection capability in vitro. We found that BCAAs can down-regulate metacyclogenesis, inhibiting Pro-dependent differentiation. Furthermore, we evaluated the ability of all three BCAAs to influence the differentiation of intracellular stages and found that they could modulate the release of trypomastigotes from infected host cells. These findings suggest that BCAAs may have an important role in the complex life cycle of T. cruzi. Thus, enzymes of their metabolism and other interacting proteins could be potential targets for the development of new therapeutic strategies for Chagas disease. Competing Interests: Declaration of competing interest On the behalf of the authors of the manuscript entitled ‘The Branched Chain Amino Acids (BCAAs) modulate the intracellular differentiation processes in Trypanosoma cruzi’, Ludmila N. Rapado, Janaina F. Nascimento, N. Carolina Manchola, Flavia S. Damasceno, Renan W. Achjian I declare that there is no financial/personal interest that could affect the interpretations of our results. (Copyright © 2023 Elsevier Inc. All rights reserved.) |
| المشرفين على المادة: | 0 (Amino Acids, Branched-Chain) GMW67QNF9C (Leucine) 0 (Protozoan Proteins) |
| تواريخ الأحداث: | Date Created: 20231108 Date Completed: 20231216 Latest Revision: 20231216 |
| رمز التحديث: | 20231217 |
| DOI: | 10.1016/j.exppara.2023.108642 |
| PMID: | 37939824 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder كن أول من يترك تعليقا!