دورية أكاديمية
A pipeline for STED super-resolution imaging and Imaris analysis of nanoscale synapse organization in mouse cortical brain slices.
| العنوان: | A pipeline for STED super-resolution imaging and Imaris analysis of nanoscale synapse organization in mouse cortical brain slices. |
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| المؤلفون: | Kruzich E; Neurobiology Section in the Department of Biology, Boston University, Boston, MA 02215, USA. Electronic address: ezrak@bu.edu., Phadke RA; Molecular Biology, Cell Biology, and Biochemistry Section in the Department of Biology, Boston University, Boston, MA 02215, USA., Brack A; Molecular Biology, Cell Biology, and Biochemistry Section in the Department of Biology, Boston University, Boston, MA 02215, USA., Stroumbakis D; Neurobiology Section in the Department of Biology, Boston University, Boston, MA 02215, USA., Infante O; Montclair State University, Montclair, NJ 07043, USA; Summer Undergraduate Research Fellowship Program, Boston University, Boston, MA 02215, USA., Cruz-Martín A; Neurobiology Section in the Department of Biology, Boston University, Boston, MA 02215, USA; Molecular Biology, Cell Biology, and Biochemistry Section in the Department of Biology, Boston University, Boston, MA 02215, USA. Electronic address: martini.cruz@gmail.com. |
| المصدر: | STAR protocols [STAR Protoc] 2023 Dec 15; Vol. 4 (4), pp. 102707. Date of Electronic Publication: 2023 Nov 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Microscopy*/methods , Dendrites*, Mice ; Animals ; Brain/diagnostic imaging ; Central Nervous System ; Synapses |
| مستخلص: | Advances in super-resolution imaging enable us to delve into its intricate structural and functional complexities with unprecedented detail. Here, we present a pipeline to visualize and analyze the nanoscale organization of cortical layer 1 apical dendritic spines in the mouse prefrontal cortex. We describe steps for brain slice preparation, immunostaining, stimulated emission depletion super-resolution microscopy, and data analysis using the Imaris software package. This protocol allows the study of physiologically relevant brain circuits implicated in neuropsychiatric disorders. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| معلومات مُعتمدة: | R01 MH129732 United States MH NIMH NIH HHS |
| فهرسة مساهمة: | Keywords: Cell Biology; Microscopy; Neuroscience |
| تواريخ الأحداث: | Date Created: 20231110 Date Completed: 20240311 Latest Revision: 20240311 |
| رمز التحديث: | 20240311 |
| مُعرف محوري في PubMed: | PMC10658395 |
| DOI: | 10.1016/j.xpro.2023.102707 |
| PMID: | 37948187 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2023.102707 |