دورية أكاديمية
In vitro and in vivo calibration of low affinity genetic Ca 2+ indicators.
| العنوان: | In vitro and in vivo calibration of low affinity genetic Ca 2+ indicators. |
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| المؤلفون: | Delrio-Lorenzo A; Unidad de Excelencia, Instituto de Biomedicina y Genética Molecular de Valladolid (IBGM), Universidad de Valladolid y Consejo Superior de Investigaciones Científicas (CSIC), c/ Sanz y Forés 3, Valladolid 47003, Spain., Rojo-Ruiz J; Unidad de Excelencia, Instituto de Biomedicina y Genética Molecular de Valladolid (IBGM), Universidad de Valladolid y Consejo Superior de Investigaciones Científicas (CSIC), c/ Sanz y Forés 3, Valladolid 47003, Spain., Torres-Vidal P; Unidad de Excelencia, Instituto de Biomedicina y Genética Molecular de Valladolid (IBGM), Universidad de Valladolid y Consejo Superior de Investigaciones Científicas (CSIC), c/ Sanz y Forés 3, Valladolid 47003, Spain., Alonso MT; Unidad de Excelencia, Instituto de Biomedicina y Genética Molecular de Valladolid (IBGM), Universidad de Valladolid y Consejo Superior de Investigaciones Científicas (CSIC), c/ Sanz y Forés 3, Valladolid 47003, Spain. Electronic address: talonso@uva.es., García-Sancho J; Unidad de Excelencia, Instituto de Biomedicina y Genética Molecular de Valladolid (IBGM), Universidad de Valladolid y Consejo Superior de Investigaciones Científicas (CSIC), c/ Sanz y Forés 3, Valladolid 47003, Spain. |
| المصدر: | Cell calcium [Cell Calcium] 2024 Jan; Vol. 117, pp. 102819. Date of Electronic Publication: 2023 Nov 08. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 8006226 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-1991 (Electronic) Linking ISSN: 01434160 NLM ISO Abbreviation: Cell Calcium Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2003->: Amsterdam : Elsevier Original Publication: [Edinburgh, New York] Churchill Livingston |
| مواضيع طبية MeSH: | Organelles*/metabolism , Aequorin*/metabolism, Mice ; Animals ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Calibration ; Sarcoplasmic Reticulum/metabolism ; Calcium/metabolism ; Calcium Signaling |
| مستخلص: | Calcium is a universal intracellular messenger and proper Ca 2+ concentrations ([Ca 2+ ]) both in the cytosol and in the lumen of cytoplasmic organelles are essential for cell functions. Ca 2+ homeostasis is achieved by a delicate pump/leak balance both at the plasma membrane and at the endomembranes, and improper Ca 2+ levels result in malfunction and disease. Selective intraorganellar Ca 2+ measurements are best achieved by using targeted genetically encoded Ca 2+ indicators (GECIs) but to calibrate the luminal fluorescent signals into accurate [Ca 2+ ] is challenging, especially in vivo, due to the difficulty to normalize and calibrate the fluorescent signal in various tissues or conditions. We report here a procedure to calibrate the ratiometric signal of GAP (GFP-Aequorin Protein) targeted to the endo-sarcoplasmic reticulum (ER/SR) into [Ca 2+ ] Competing Interests: Declaration of Competing Interest The authors declare that there are no competing interests associated with the manuscript. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Calcium imaging; Drosophila melanogaster; Fluorescence; GAP; GECI; SERCA |
| المشرفين على المادة: | 147336-22-9 (Green Fluorescent Proteins) 50934-79-7 (Aequorin) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 20231113 Date Completed: 20240116 Latest Revision: 20240212 |
| رمز التحديث: | 20240213 |
| DOI: | 10.1016/j.ceca.2023.102819 |
| PMID: | 37956535 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1532-1991 |
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| DOI: | 10.1016/j.ceca.2023.102819 |