دورية أكاديمية
أعرض في EDS

Registration of Functioning of a Single Horseradish Peroxidase Macromolecule with a Solid-State Nanopore.

التفاصيل البيبلوغرافية
العنوان: Registration of Functioning of a Single Horseradish Peroxidase Macromolecule with a Solid-State Nanopore.
المؤلفون: Ivanov YD; Institute of Biomedical Chemistry, 10, Pogodinskaya St., Moscow 119121, Russia., Ableev AN; Institute of Biomedical Chemistry, 10, Pogodinskaya St., Moscow 119121, Russia., Shumov ID; Institute of Biomedical Chemistry, 10, Pogodinskaya St., Moscow 119121, Russia., Ivanova IA; Institute of Biomedical Chemistry, 10, Pogodinskaya St., Moscow 119121, Russia., Vaulin NV; Laboratory of Renewable Energy Sources, St. Petersburg Academic University, 8/3, Khlopina st., St. Petersburg 194021, Russia.; Institute for Analytical Instrumentation RAS, 31-33 Lit. A, Ivana Chernykh St., St. Petersburg 198095, Russia., Lebedev DV; Laboratory of Renewable Energy Sources, St. Petersburg Academic University, 8/3, Khlopina st., St. Petersburg 194021, Russia.; Institute for Analytical Instrumentation RAS, 31-33 Lit. A, Ivana Chernykh St., St. Petersburg 198095, Russia.; Institute of Chemistry, Saint Petersburg State University, 7/9, Universitetskaya Nab., St. Petersburg 199034, Russia., Bukatin AS; Laboratory of Renewable Energy Sources, St. Petersburg Academic University, 8/3, Khlopina st., St. Petersburg 194021, Russia.; Institute for Analytical Instrumentation RAS, 31-33 Lit. A, Ivana Chernykh St., St. Petersburg 198095, Russia., Mukhin IS; Laboratory of Renewable Energy Sources, St. Petersburg Academic University, 8/3, Khlopina st., St. Petersburg 194021, Russia.; Higher School of Engineering Physics, Peter the Great Polytechnic University, 26, Polytehnicheskaya St., St. Petersburg 194021, Russia., Archakov AI; Institute of Biomedical Chemistry, 10, Pogodinskaya St., Moscow 119121, Russia.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2023 Oct 27; Vol. 24 (21). Date of Electronic Publication: 2023 Oct 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Nanopores*, Horseradish Peroxidase/chemistry ; Sulfonic Acids/chemistry ; Benzothiazoles/chemistry ; Macromolecular Substances
مستخلص: Currently, nanopore-based technology for the determination of the functional activity of single enzyme molecules continues its development. The use of natural nanopores for studying single enzyme molecules is known. At that, the approach utilizing artificial solid-state nanopores is also promising but still understudied. Herein, we demonstrate the use of a nanotechnology-based approach for the investigation of the enzymatic activity of a single molecule of horseradish peroxidase with a solid-state nanopore. The artificial 5 nm solid-state nanopore has been formed in a 40 nm thick silicon nitride structure. A single molecule of HRP has been entrapped into the nanopore. The activity of the horseradish peroxidase (HRP) enzyme molecule inserted in the nanopore has been monitored by recording the time dependence of the ion current through the nanopore in the course of the reaction of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) oxidation reaction. We have found that in the process of ABTS oxidation in the presence of 2.5 mM hydrogen peroxide, individual HRP enzyme molecules are able to retain activity for approximately 700 s before a decrease in the ion current through the nanopore, which can be explained by structural changes of the enzyme.
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معلومات مُعتمدة: 075-15-2021-933 The Ministry of Education and Science of the Russian Federation
فهرسة مساهمة: Keywords: enzymatic activity; horseradish peroxidase; nanopore detector; solid-state nanopore
المشرفين على المادة: 28752-68-3 (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid)
EC 1.11.1.- (Horseradish Peroxidase)
0 (Sulfonic Acids)
0 (Benzothiazoles)
0 (Macromolecular Substances)
تواريخ الأحداث: Date Created: 20231114 Date Completed: 20231115 Latest Revision: 20231117
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10647385
DOI: 10.3390/ijms242115636
PMID: 37958620
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms242115636