دورية أكاديمية
Target-Guided Isolation and Purification of Antioxidants from Urtica laetevirens Maxim. by HSCCC Combined with Online DPPH-HPLC Analysis.
| العنوان: | Target-Guided Isolation and Purification of Antioxidants from Urtica laetevirens Maxim. by HSCCC Combined with Online DPPH-HPLC Analysis. |
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| المؤلفون: | Li A; School of Life Science, Qinghai Normal University, Xining 810008, China., La M; School of Life Science, Qinghai Normal University, Xining 810008, China., Wang H; School of Life Science, Qinghai Normal University, Xining 810008, China., Zhao J; Agricultural and Rural Science and Technology Guidance Development Service Center of Qinghai Province, Xining 810008, China., Wang Y; School of Life Science, Qinghai Normal University, Xining 810008, China., Mian R; School of Life Science, Qinghai Normal University, Xining 810008, China., He F; School of Life Science, Qinghai Normal University, Xining 810008, China., Wang Y; School of Life Science, Qinghai Normal University, Xining 810008, China., Yang T; School of Life Science, Qinghai Normal University, Xining 810008, China., Zou D; School of Life Science, Qinghai Normal University, Xining 810008, China.; Agricultural and Rural Science and Technology Guidance Development Service Center of Qinghai Province, Xining 810008, China. |
| المصدر: | Molecules (Basel, Switzerland) [Molecules] 2023 Oct 29; Vol. 28 (21). Date of Electronic Publication: 2023 Oct 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, c1995- |
| مواضيع طبية MeSH: | Antioxidants*/chemistry , Urticaceae*, Chromatography, High Pressure Liquid/methods ; Plant Extracts/chemistry ; Magnetic Resonance Spectroscopy ; Countercurrent Distribution/methods |
| مستخلص: | Urtica laetevirens Maxim. is used extensively in traditional Chinese medicine (TCM) for its potent antioxidative properties. In this study, three antioxidants were purified from U. laetevirens. using HSCCC guided by online DPPH-HPLC analysis. Firstly, the online DPPH-HPLC analysis was performed to profile out the antioxidant active molecules in U. laetevirens. The ultrasonic-assisted extraction conditions were optimized by response surface methodology and the results showed the targeted antioxidant active molecules could be well enriched under the optimized extraction conditions. Then, the antioxidant active molecules were separated by high-speed countercurrent chromatography ethyl acetate/ n -butanol/water (2:3:5, v / v / v ) as the solvent system. Finally, the three targets including 16.8 mg of Isovitexin, 9.8 mg of Isoorientin, and 26.7 mg of Apigenin-6,8-di-C-β-d-glucopyranoside were obtained from 100 mg of sample. Their structures were identified by 1 H NMR spectroscopy. |
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| معلومات مُعتمدة: | 32260129 National Natural Science Foundation of China; 82304892 National Natural Science Foundation of China; 2021-ZJ-976Q General Program of Natural Science Foundation of Qinghai Province |
| فهرسة مساهمة: | Keywords: HSCCC; Urtica laetevirens Maxim.; antioxidant active molecules; online HPLC-DPPH; response surface methodology |
| المشرفين على المادة: | 0 (Antioxidants) 0 (Plant Extracts) |
| تواريخ الأحداث: | Date Created: 20231114 Date Completed: 20231115 Latest Revision: 20231117 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC10650309 |
| DOI: | 10.3390/molecules28217332 |
| PMID: | 37959752 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1420-3049 |
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| DOI: | 10.3390/molecules28217332 |