Phase separation of S-RNase promotes self-incompatibility in Petunia hybrida.

التفاصيل البيبلوغرافية
العنوان:	Phase separation of S-RNase promotes self-incompatibility in Petunia hybrida.
المؤلفون:	Tian H; The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing, 100101, China.; University of the Chinese Academy of Sciences, Beijing, 100049, China., Zhang H; University of the Chinese Academy of Sciences, Beijing, 100049, China.; Beijing Institute of Genomics, Chinese Academy of Sciences, National Center for Bioinformation, Beijing, 100101, China., Huang H; The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing, 100101, China., Zhang Y; The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing, 100101, China., Xue Y; The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing, 100101, China.; University of the Chinese Academy of Sciences, Beijing, 100049, China.; Beijing Institute of Genomics, Chinese Academy of Sciences, National Center for Bioinformation, Beijing, 100101, China.
المصدر:	Journal of integrative plant biology [J Integr Plant Biol] 2024 May; Vol. 66 (5), pp. 986-1006. Date of Electronic Publication: 2024 Jan 02.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Wiley-Blackwell Pub Country of Publication: China (Republic : 1949- ) NLM ID: 101250502 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1744-7909 (Electronic) Linking ISSN: 16729072 NLM ISO Abbreviation: J Integr Plant Biol Subsets: MEDLINE
أسماء مطبوعة:	Publication: [China] : Wiley-Blackwell Pub Original Publication: [Carlton South, Victoria] : Blackwell Pub., 2005-
مواضيع طبية MeSH:	Petunia/genetics , Petunia/metabolism , Ribonucleases/metabolism , Ribonucleases/genetics , Self-Incompatibility in Flowering Plants/genetics , Pollen Tube/growth & development , Pollen Tube/metabolism , Pollen Tube/genetics , Plant Proteins/metabolism , Plant Proteins/genetics, Flowers/genetics ; Phase Separation
مستخلص:	Self-incompatibility (SI) is an intraspecific reproductive barrier widely present in angiosperms. The SI system with the broadest occurrence in angiosperms is based on an S-RNase linked to a cluster of multiple S-locus F-box (SLF) genes found in the Solanaceae, Plantaginaceae, Rosaceae, and Rutaceae. Recent studies reveal that non-self S-RNase is degraded by the Skip Cullin F-box (SCF) ^SLF -mediated ubiquitin-proteasome system in a collaborative manner in Petunia, but how self-RNase functions largely remains mysterious. Here, we show that S-RNases form S-RNase condensates (SRCs) in the self-pollen tube cytoplasm through phase separation and the disruption of SRC formation breaks SI in self-incompatible Petunia hybrida. We further find that the pistil SI factors of a small asparagine-rich protein HT-B and thioredoxin h together with a reduced state of the pollen tube all promote the expansion of SRCs, which then sequester several actin-binding proteins, including the actin polymerization factor PhABRACL, the actin polymerization activity of which is reduced by S-RNase in vitro. Meanwhile, we find that S-RNase variants lacking condensation ability fail to recruit PhABRACL and are unable to induce actin foci formation required for pollen tube growth inhibition. Taken together, our results demonstrate that phase separation of S-RNase promotes SI response in P. hybrida, revealing a new mode of S-RNase action. (© 2023 The Authors. Journal of Integrative Plant Biology published by John Wiley & Sons Australia, Ltd on behalf of Institute of Botany, Chinese Academy of Sciences.)
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معلومات مُعتمدة:	32030007 National Natural Science Foundation of China
فهرسة مساهمة:	Keywords: S‐RNase; phase separation; self‐incompatibility
المشرفين على المادة:	EC 3.1.- (Ribonucleases) EC 3.1.4.- (ribonuclease S) 0 (Plant Proteins)
تواريخ الأحداث:	Date Created: 20231114 Date Completed: 20240527 Latest Revision: 20240527
رمز التحديث:	20240527
DOI:	10.1111/jipb.13584
PMID:	37963073
قاعدة البيانات:	MEDLINE

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تدمد:	1744-7909
DOI:	10.1111/jipb.13584