دورية أكاديمية
Differentiating the roles of proteins and polysomes in nucleoid size homeostasis in Escherichia coli.
| العنوان: | Differentiating the roles of proteins and polysomes in nucleoid size homeostasis in Escherichia coli. |
|---|---|
| المؤلفون: | Chang MH; Department of Physics and Astronomy, The University of Tennessee, Knoxville, Tennessee., Lavrentovich MO; Department of Physics and Astronomy, The University of Tennessee, Knoxville, Tennessee; Department of Earth, Environment, and Physics, Worcester State University, Worcester, Massachusetts. Electronic address: mlavrent@utk.edu., Männik J; Department of Physics and Astronomy, The University of Tennessee, Knoxville, Tennessee. Electronic address: jmannik@utk.edu. |
| المصدر: | Biophysical journal [Biophys J] 2024 Jun 04; Vol. 123 (11), pp. 1435-1448. Date of Electronic Publication: 2023 Nov 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 0370626 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1542-0086 (Electronic) Linking ISSN: 00063495 NLM ISO Abbreviation: Biophys J Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge, MA : Cell Press Original Publication: New York, Published by Rockefeller University Press [etc.] for the Biophysical Society. |
| مواضيع طبية MeSH: | Escherichia coli*/metabolism , Escherichia coli*/cytology , Homeostasis* , Polyribosomes*/metabolism , Escherichia coli Proteins*/metabolism , Models, Biological* |
| مستخلص: | A defining feature of the bacterial cytosolic interior is a distinct membrane-less organelle, the nucleoid, that contains the chromosomal DNA. Although increasing experimental evidence indicates that macromolecular crowding is the dominant mechanism for nucleoid formation, it has remained unclear which crowders control nucleoid volume. It is commonly assumed that polyribosomes play a dominant role, yet the volume fraction of soluble proteins in the cytosol is comparable with that of polyribosomes. Here, we develop a free energy-based model for the cytosolic interior of a bacterial cell to distinguish contributions arising from polyribosomes and cytosolic proteins in nucleoid volume control. The parameters of the model are determined from the existing experimental data. We show that, while the polysomes establish the existence of the nucleoid as a distinct phase, the proteins control the nucleoid volume in physiologically relevant conditions. Our model explains experimental findings in Escherichia coli that the nucleoid compaction curves in osmotic shock measurements do not depend on cell growth rate and that dissociation of polysomes in slow growth rates does not lead to significant nucleoid expansion, while the nucleoid phase disappears in fastest growth rates. Furthermore, the model predicts a cross-over in the exclusion of crowders by their linear dimensions from the nucleoid phase: below the cross-over of 30-50 nm, the concentration of crowders in the nucleoid phase decreases linearly as a function of the crowder diameter, while decreasing exponentially above the cross-over size. Our work points to the possibility that bacterial cells maintain nucleoid size and protein concentration homeostasis via feedback in which protein concentration controls nucleoid dimensions and the nucleoid dimensions control protein synthesis rate. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 Biophysical Society. Published by Elsevier Inc. All rights reserved.) |
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| معلومات مُعتمدة: | R01 GM127413 United States GM NIGMS NIH HHS |
| المشرفين على المادة: | 0 (Escherichia coli Proteins) |
| تواريخ الأحداث: | Date Created: 20231117 Date Completed: 20240605 Latest Revision: 20240612 |
| رمز التحديث: | 20240612 |
| مُعرف محوري في PubMed: | PMC11163298 |
| DOI: | 10.1016/j.bpj.2023.11.010 |
| PMID: | 37974398 |
| قاعدة البيانات: | MEDLINE |
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