دورية أكاديمية
The 3D organization of the mineralized scales of the sturgeon has structures reminiscent of dentin and bone: A FIB-SEM study.
| العنوان: | The 3D organization of the mineralized scales of the sturgeon has structures reminiscent of dentin and bone: A FIB-SEM study. |
|---|---|
| المؤلفون: | Milgram J; Hebrew University Jerusalem, Faculty of Agriculture Food & Environment, Koret School of Veterinary Medicine, P.O.B. 12, Rehovot 7610001, Israel. Electronic address: josh.milgram@mail.huji.ac.il., Rehav K; Department of Chemical Research Support, Weizmann Institute of Science, Rehovot 7610001, Israel. Electronic address: katya.rechav@weizmann.ac.il., Ibrahim J; Archaeological Science Unit, Weizmann Institute of Science, Rehovot 7610001, Israel. Electronic address: jamal.ibrahim@weizmann.ac.il., Shahar R; Hebrew University Jerusalem, Faculty of Agriculture Food & Environment, Koret School of Veterinary Medicine, P.O.B. 12, Rehovot 7610001, Israel. Electronic address: ron.shahar1@mail.huji.ac.il., Weiner S; Archaeological Science Unit, Weizmann Institute of Science, Rehovot 7610001, Israel; Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot, 7610001, Israel. Electronic address: steve.weiner@weizmann.ac.il. |
| المصدر: | Journal of structural biology [J Struct Biol] 2023 Dec; Vol. 215 (4), pp. 108045. Date of Electronic Publication: 2023 Nov 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 9011206 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-8657 (Electronic) Linking ISSN: 10478477 NLM ISO Abbreviation: J Struct Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: San Diego : Academic Press, c1990- |
| مواضيع طبية MeSH: | Volume Electron Microscopy* , Collagen*, Animals ; Microscopy, Electron ; Bone and Bones ; Dentin ; Microscopy, Electron, Scanning |
| مستخلص: | Scales are structures composed of mineralized collagen fibrils embedded in the skin of fish. Here we investigate structures contributing to the bulk of the scale material of the sturgeon (Acipencer guldenstatii) at the millimeter, micrometer and nanometer length scales. Polished and fracture surfaces were prepared in each of the three anatomic planes for imaging with light and electron microscopy, as well as focused ion beam - scanning electron microscopy (FIB-SEM). The scale is composed of three layers, upper and lower layers forming the bulk of the scale, as well as a thin surface layer. FTIR shows that the scale is composed mainly of collagen and carbonated hydroxyapatite. Lacunae are present throughout the structure. Fracture surfaces of all three layers are characterized by large diameter collagen fibril bundles (CFBs) emanating from a plane comprising smaller diameter CFBs orientated in different directions. Fine lineations seen in polished surfaces of both major layers are used to define planes called here the striation planes. FIB-SEM image stacks of the upper and lower layers acquired in planes aligned with the striation planes, show that CFBs are oriented in various directions within the striation plane, with larger CFBs emanating from the striation plane. Fibril bundles oriented in different directions in the same plane is reminiscent of a similar organization in orthodentin. The large collagen fibril bundles emanating out of this plane are analogous to von Korff fibrils found in developing dentin with respect to size and orientation. Scales of the sturgeon are unusual in that their mineralized collagen fibril organization contains structural elements of both dentin and bone. The sturgeon scale may be an example of an early evolved mineralized material which is neither bone nor dentin but contains characteristics of both materials, however, the fossil data required to confirm this is missing. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bone; Dentin; FIB-SEM; Fish; Scale; Sturgeon |
| المشرفين على المادة: | 9007-34-5 (Collagen) |
| تواريخ الأحداث: | Date Created: 20231117 Date Completed: 20231206 Latest Revision: 20231211 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jsb.2023.108045 |
| PMID: | 37977509 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-8657 |
|---|---|
| DOI: | 10.1016/j.jsb.2023.108045 |