دورية أكاديمية
Structural elucidation of novel pro-inflammatory polysaccharides from Daphne mezereum L.
| العنوان: | Structural elucidation of novel pro-inflammatory polysaccharides from Daphne mezereum L. |
|---|---|
| المؤلفون: | Butt HS; Section for Pharmaceutical Chemistry, Department of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, NO-0316 Oslo, Norway. Electronic address: h.s.butt@farmasi.uio.no., Ulriksen ES; Department of Pharmacology, Institute of Clinical Medicine, University of Oslo, P.O. Box 1057, Blindern, NO-0316 Oslo, Norway., Rise F; Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, NO-0315 Oslo, Norway., Wangensteen H; Section for Pharmaceutical Chemistry, Department of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, NO-0316 Oslo, Norway., Duus JØ; Department of Chemistry, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark., Inngjerdingen M; Department of Pharmacology, Institute of Clinical Medicine, University of Oslo, P.O. Box 1057, Blindern, NO-0316 Oslo, Norway., Inngjerdingen KT; Section for Pharmaceutical Chemistry, Department of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, NO-0316 Oslo, Norway. |
| المصدر: | Carbohydrate polymers [Carbohydr Polym] 2024 Jan 15; Vol. 324, pp. 121554. Date of Electronic Publication: 2023 Nov 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 8307156 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1344 (Electronic) Linking ISSN: 01448617 NLM ISO Abbreviation: Carbohydr Polym Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <1992-> : Barking : Elsevier Applied Science Publishers Original Publication: London [Eng.] : Applied Science Publishers, c1981- |
| مواضيع طبية MeSH: | Daphne*, Leukocytes, Mononuclear ; Rhamnogalacturonans ; Polysaccharides/pharmacology ; Polysaccharides/chemistry ; Pectins/chemistry ; Gas Chromatography-Mass Spectrometry |
| مستخلص: | Daphne mezereum L., an important medicinal plant in Scandinavian folk medicine, was used to treat ailments such as diarrhea, swelling and stomach pain. A range of natural compounds have been isolated, but little attention has been given to the polysaccharides in this plant. Previous work in our group have shown that a polysaccharide enriched fraction from the bark of D. mezereum exhibited pro-inflammatory effects. To pursue this further, the aim of the present work was to isolate and characterize these polysaccharides. From the ethanol-precipitate of a water extract, one neutral (DMP-NF) and one acidic (DMP-AF) fraction was isolated by anion-exchange chromatography. GC, GC-MS and 1D- and 2D-NMR were used to characterize the polysaccharide structures. DMP-NF appeared to be a mixture of arabinan, arabinogalactan and hemicelluloses such as xyloglucan, mannan and xylan. DMP-AF contained a pectic polysaccharide mainly consisting of an unusually long homogalacturonan backbone. Enzymatic treatment by pectinase of DMP-AF yielded DMP-ED, which contained a rhamnogalacturonan-I backbone with arabinan, galactan and arabinogalactan side chains. Both DMP-NF and DMP-ED induced IFN-γ and TNF-α secretion in peripheral blood mononuclear cells (PBMCs), DMP-ED being the most potent fraction. DMP-AF was less active, which might be due to a less sterically available rhamnogalacturonan-I domain. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Daphne mezereum; IFN-γ; PBMC; Pectin; Polysaccharides; TNF-α |
| المشرفين على المادة: | 0 (Rhamnogalacturonans) 0 (Polysaccharides) 89NA02M4RX (Pectins) |
| تواريخ الأحداث: | Date Created: 20231120 Date Completed: 20231122 Latest Revision: 20231122 |
| رمز التحديث: | 20231122 |
| DOI: | 10.1016/j.carbpol.2023.121554 |
| PMID: | 37985118 |
| قاعدة البيانات: | MEDLINE |
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