دورية أكاديمية
Broad receptor tropism and immunogenicity of a clade 3 sarbecovirus.
| العنوان: | Broad receptor tropism and immunogenicity of a clade 3 sarbecovirus. |
|---|---|
| المؤلفون: | Lee J; Department of Biochemistry, University of Washington, Seattle, WA, USA., Zepeda SK; Department of Biochemistry, University of Washington, Seattle, WA, USA., Park YJ; Department of Biochemistry, University of Washington, Seattle, WA, USA; Howard Hughes Medical Institute, Seattle, WA 98195, USA., Taylor AL; Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA., Quispe J; Department of Biochemistry, University of Washington, Seattle, WA, USA., Stewart C; Department of Biochemistry, University of Washington, Seattle, WA, USA., Leaf EM; Department of Biochemistry, University of Washington, Seattle, WA, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Treichel C; Department of Biochemistry, University of Washington, Seattle, WA, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Corti D; Humabs Biomed SA, a Subsidiary of Vir. Biotechnology, 6500 Bellinzona, Switzerland., King NP; Department of Biochemistry, University of Washington, Seattle, WA, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Starr TN; Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA., Veesler D; Department of Biochemistry, University of Washington, Seattle, WA, USA; Howard Hughes Medical Institute, Seattle, WA 98195, USA. Electronic address: dveesler@uw.edu. |
| المصدر: | Cell host & microbe [Cell Host Microbe] 2023 Dec 13; Vol. 31 (12), pp. 1961-1973.e11. Date of Electronic Publication: 2023 Nov 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101302316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1934-6069 (Electronic) Linking ISSN: 19313128 NLM ISO Abbreviation: Cell Host Microbe Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, Mass. : Cell Press |
| مواضيع طبية MeSH: | Severe acute respiratory syndrome-related coronavirus* , Chiroptera*, Animals ; Humans ; Angiotensin-Converting Enzyme 2 ; SARS-CoV-2/genetics ; Tropism ; Spike Glycoprotein, Coronavirus ; Antibodies, Viral |
| مستخلص: | Although Rhinolophus bats harbor diverse clade 3 sarbecoviruses, the structural determinants of receptor tropism along with the antigenicity of their spike (S) glycoproteins remain uncharacterized. Here, we show that the African Rhinolophus bat clade 3 sarbecovirus PRD-0038 S has a broad angiotensin-converting enzyme 2 (ACE2) usage and that receptor-binding domain (RBD) mutations further expand receptor promiscuity and enable human ACE2 utilization. We determine a cryo-EM structure of the PRD-0038 RBD bound to Rhinolophus alcyone ACE2, explaining receptor tropism and highlighting differences with SARS-CoV-1 and SARS-CoV-2. Characterization of PRD-0038 S using cryo-EM and monoclonal antibody reactivity reveals its distinct antigenicity relative to SARS-CoV-2 and identifies PRD-0038 cross-neutralizing antibodies for pandemic preparedness. PRD-0038 S vaccination elicits greater titers of antibodies cross-reacting with vaccine-mismatched clade 2 and clade 1a sarbecoviruses compared with SARS-CoV-2 S due to broader antigenic targeting, motivating the inclusion of clade 3 antigens in next-generation vaccines for enhanced resilience to viral evolution. Competing Interests: Declaration of interests N.P.K. and D.V. are named as inventors on patents for coronavirus nanoparticle vaccines filed by the University of Washington. N.P.K. is a co-founder, shareholder, paid consultant, and chair of the scientific advisory board of Icosavax, Inc. and has received an unrelated sponsored research agreement from Pfizer. D.C. is an employee of Vir Biotechnology and may hold shares in Vir Biotechnology. T.N.S. consults for Apriori Bio on DMS. The lab of T.N.S. has received sponsored research agreements unrelated to the present work from Vir Biotechnology and Aerium Therapeutics, Inc. T.N.S. may receive a share of intellectual property revenue as inventor on a Fred Hutchinson Cancer Center-optioned patent related to stabilization of SARS-CoV-2 RBDs. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| التعليقات: | Update of: bioRxiv. 2023 Sep 13;:. (PMID: 37745523) |
| References: | Science. 2021 Aug 6;373(6555):648-654. (PMID: 34210893) Nature. 2022 Dec;612(7941):748-757. (PMID: 36477529) Elife. 2023 Mar 21;12:. (PMID: 36942851) PLoS Pathog. 2022 Feb 8;18(2):e1010248. (PMID: 35134084) Nature. 2020 Aug;584(7821):437-442. (PMID: 32555388) Nature. 2020 Mar;579(7798):270-273. (PMID: 32015507) Science. 2022 Aug 12;377(6607):735-742. (PMID: 35857703) Science. 2021 Aug 27;373(6558):991-998. (PMID: 34214046) Science. 2022 Aug 5;377(6606):eabq0839. (PMID: 35857620) N Engl J Med. 2003 May 15;348(20):1953-66. (PMID: 12690092) Science. 2022 Jan 28;375(6579):449-454. (PMID: 34990214) Nature. 2023 Feb;614(7948):521-529. (PMID: 36535326) Cell. 2020 Nov 12;183(4):1070-1085.e12. (PMID: 33031744) Structure. 2019 Jan 2;27(1):134-139.e3. (PMID: 30344107) Science. 2022 Jul 22;377(6604):420-424. (PMID: 35762884) J Open Source Softw. 2019;4(44):. (PMID: 31897449) Nature. 2020 Jul;583(7815):290-295. (PMID: 32422645) Nat Struct Mol Biol. 2015 Nov;22(11):833-4. (PMID: 26581513) Nature. 2021 Apr;592(7854):438-443. (PMID: 33690265) N Engl J Med. 2020 Feb 20;382(8):727-733. (PMID: 31978945) Nature. 2022 Feb;602(7898):664-670. (PMID: 35016195) J Comput Chem. 2004 Oct;25(13):1605-12. (PMID: 15264254) Virus Evol. 2021 Feb 05;7(1):veab007. (PMID: 33754082) Nat Methods. 2017 Mar;14(3):290-296. (PMID: 28165473) Science. 2021 Feb 12;371(6530):735-741. (PMID: 33436524) Cell. 2021 Apr 1;184(7):1804-1820.e16. (PMID: 33691139) Elife. 2016 Sep 26;5:. (PMID: 27669148) Science. 2022 Aug 19;377(6608):890-894. (PMID: 35857529) Emerg Infect Dis. 2021 Apr;27(4):. (PMID: 33567247) Cell Rep. 2022 May 17;39(7):110812. (PMID: 35568025) Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21. (PMID: 20057044) Cell. 2020 Apr 16;181(2):281-292.e6. (PMID: 32155444) Science. 2022 Nov 11;378(6620):619-627. (PMID: 36264829) Nature. 2003 Nov 27;426(6965):450-4. (PMID: 14647384) PLoS Pathog. 2017 Nov 30;13(11):e1006698. (PMID: 29190287) Cell Res. 2022 Mar;32(3):315-318. (PMID: 35017654) Nature. 2022 Mar;603(7902):706-714. (PMID: 35104837) Virol Sin. 2018 Feb;33(1):104-107. (PMID: 29500691) Nature. 2013 Nov 28;503(7477):535-8. (PMID: 24172901) Nature. 2022 Aug;608(7923):593-602. (PMID: 35714668) Int J Infect Dis. 2023 Jun;131:57-64. (PMID: 36870470) PLoS Pathog. 2022 Nov 18;18(11):e1010951. (PMID: 36399443) N Engl J Med. 2003 May 15;348(20):1967-76. (PMID: 12690091) Bio Protoc. 2016 Dec 5;6(23):. (PMID: 28018942) Science. 2020 Sep 18;369(6510):1501-1505. (PMID: 32703906) IUCrJ. 2019 Jan 01;6(Pt 1):5-17. (PMID: 30713699) Nature. 2021 Sep;597(7874):97-102. (PMID: 34261126) J Virol. 2020 Sep 29;94(20):. (PMID: 32699095) Nature. 2021 Sep;597(7874):103-108. (PMID: 34280951) J Mol Biol. 2003 Oct 31;333(4):721-45. (PMID: 14568533) Microbiol Resour Announc. 2019 Jul 11;8(28):. (PMID: 31296683) Cell Rep. 2023 Apr 25;42(4):112326. (PMID: 37000623) Science. 2020 Nov 6;370(6517):725-730. (PMID: 32958580) Nat Microbiol. 2022 Aug;7(8):1161-1179. (PMID: 35798890) EMBO J. 2022 Jan 4;41(1):e109962. (PMID: 34981520) Sci Rep. 2021 Jul 19;11(1):14723. (PMID: 34282196) Cell Rep. 2021 Sep 28;36(13):109760. (PMID: 34534459) J Virol. 2015 Dec 30;90(6):3253-6. (PMID: 26719272) J Mol Biol. 2007 Sep 21;372(3):774-97. (PMID: 17681537) Nat Microbiol. 2020 Apr;5(4):562-569. (PMID: 32094589) J Virol. 2010 Nov;84(21):11336-49. (PMID: 20686038) Science. 2021 Sep 03;373(6559):1109-1116. (PMID: 34344823) Nat Commun. 2021 Feb 9;12(1):972. (PMID: 33563978) Sci Transl Med. 2022 Jan 26;14(629):eabj7125. (PMID: 34726473) Nature. 2020 Jul;583(7815):282-285. (PMID: 32218527) Ultramicroscopy. 2013 Dec;135:24-35. (PMID: 23872039) Proc Natl Acad Sci U S A. 2016 Mar 15;113(11):3048-53. (PMID: 26976607) Nature. 2022 Apr;604(7905):330-336. (PMID: 35172323) PLoS Biol. 2022 Jul 27;20(7):e3001738. (PMID: 35895696) Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501. (PMID: 20383002) Science. 2020 Nov 20;370(6519):950-957. (PMID: 32972994) Nature. 2022 Mar;603(7903):913-918. (PMID: 35114688) Cell. 2020 Sep 3;182(5):1295-1310.e20. (PMID: 32841599) Nat Struct Mol Biol. 2021 Jun;28(6):478-486. (PMID: 33981021) Cell. 2021 Aug 19;184(17):4380-4391.e14. (PMID: 34147139) Sci Transl Med. 2021 Jun 30;13(600):. (PMID: 34103407) J Exp Med. 2021 Mar 1;218(3):. (PMID: 33211088) Emerg Infect Dis. 2009 Mar;15(3):482-5. (PMID: 19239771) Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):861-877. (PMID: 31588918) Nat Commun. 2021 Feb 5;12(1):837. (PMID: 33547281) Appl Environ Microbiol. 2007 Feb;73(4):1189-98. (PMID: 17189442) Sci Immunol. 2022 Dec 23;7(78):eadf1421. (PMID: 36356052) Lancet Reg Health Am. 2021 Sep;1:100021. (PMID: 34514463) Elife. 2018 Nov 09;7:. (PMID: 30412051) Science. 2005 Sep 16;309(5742):1864-8. (PMID: 16166518) Cell Rep. 2022 Jun 28;39(13):111009. (PMID: 35732171) Viruses. 2022 Jan 09;14(1):. (PMID: 35062318) Nat Microbiol. 2022 Nov;7(11):1756-1761. (PMID: 36195753) Nat Med. 2015 Dec;21(12):1508-13. (PMID: 26552008) Nat Commun. 2017 Apr 10;8:15092. (PMID: 28393837) Nat Methods. 2020 Dec;17(12):1214-1221. (PMID: 33257830) J Struct Biol. 2005 Jul;151(1):41-60. (PMID: 15890530) J Virol. 2010 Apr;84(7):3210-9. (PMID: 20071581) Cell. 2021 Oct 14;184(21):5432-5447.e16. (PMID: 34619077) Chem Sci. 2021 Nov 25;13(2):386-395. (PMID: 35126971) Nat Microbiol. 2023 Apr;8(4):569-580. (PMID: 37012355) Cell. 2020 Nov 12;183(4):1024-1042.e21. (PMID: 32991844) Nat Methods. 2019 Nov;16(11):1146-1152. (PMID: 31591575) Cell. 2020 Apr 16;181(2):271-280.e8. (PMID: 32142651) Science. 2022 Feb 25;375(6583):864-868. (PMID: 35076256) Science. 2021 Mar 25;:. (PMID: 33766944) Viruses. 2020 May 06;12(5):. (PMID: 32384820) Nature. 2020 May;581(7807):215-220. (PMID: 32225176) J Struct Biol. 2017 Apr;198(1):38-42. (PMID: 28254381) Protein Sci. 2018 Jan;27(1):14-25. (PMID: 28710774) Cell. 2019 Feb 21;176(5):1026-1039.e15. (PMID: 30712865) Sci Rep. 2018 Oct 24;8(1):15701. (PMID: 30356097) PLoS Pathog. 2022 Sep 22;18(9):e1010828. (PMID: 36136995) Cell. 2021 Apr 29;184(9):2332-2347.e16. (PMID: 33761326) J Struct Biol. 2012 Dec;180(3):519-30. (PMID: 23000701) |
| معلومات مُعتمدة: | DP1 AI158186 United States AI NIAID NIH HHS; F31 AI174573 United States AI NIAID NIH HHS; P01 AI167966 United States AI NIAID NIH HHS; S10 OD026959 United States OD NIH HHS; K99 AI166250 United States AI NIAID NIH HHS; S10 OD021644 United States OD NIH HHS; 75N93022C00036 United States AI NIAID NIH HHS; T32 GM008268 United States GM NIGMS NIH HHS; P30 CA042014 United States CA NCI NIH HHS |
| فهرسة مساهمة: | Keywords: ACE2; PRD-0038; SARS-CoV-2; bat coronaviruses; clade 3 sarbecovirus; cryo-EM; receptor tropism; spike glycoprotein; vaccine; zoonotic spillover |
| المشرفين على المادة: | EC 3.4.17.23 (Angiotensin-Converting Enzyme 2) 0 (Spike Glycoprotein, Coronavirus) 0 (Antibodies, Viral) |
| تواريخ الأحداث: | Date Created: 20231121 Date Completed: 20231216 Latest Revision: 20240308 |
| رمز التحديث: | 20240308 |
| مُعرف محوري في PubMed: | PMC10913562 |
| DOI: | 10.1016/j.chom.2023.10.018 |
| PMID: | 37989312 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1934-6069 |
|---|---|
| DOI: | 10.1016/j.chom.2023.10.018 |