دورية أكاديمية

Traversing the Cell Wall: The Chitinolytic Activity of Histoplasma capsulatum Extracellular Vesicles Facilitates Their Release.

التفاصيل البيبلوغرافية
العنوان: Traversing the Cell Wall: The Chitinolytic Activity of Histoplasma capsulatum Extracellular Vesicles Facilitates Their Release.
المؤلفون: Valdez AF; Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil.; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA., de Souza TN; Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil.; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA., Bonilla JJA; Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil., Zamith-Miranda D; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA., Piffer AC; Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil.; Unité Biologie des ARN des Pathogènes Fongiques, Départament de Mycologie, Institut Pasteur, Université Paris Cité, F-75015 Paris, France., Araujo GRS; Laboratório de Biofísica de Fungos, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil., Guimarães AJ; Instituto Biomédico, Departamento de Microbiologia e Parasitologia-MIP, Universidade Federal Fluminense, Niterói 24210-130, RJ, Brazil.; Rede Micologia, RJ, FAPERJ, Rio de Janeiro 21941-902, RJ, Brazil., Frases S; Laboratório de Biofísica de Fungos, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil.; Rede Micologia, RJ, FAPERJ, Rio de Janeiro 21941-902, RJ, Brazil., Pereira AK; Instituto de Química, Universidade Estadual de Campinas, Campinas, São Paulo 13083-970, SP, Brazil., Fill TP; Instituto de Química, Universidade Estadual de Campinas, Campinas, São Paulo 13083-970, SP, Brazil., Estevao IL; Department of Biological Sciences, Border Biomedical Research Center, University of Texas El Paso, El Paso, TX 79902, USA., Torres A; Department of Biological Sciences, Border Biomedical Research Center, University of Texas El Paso, El Paso, TX 79902, USA., Almeida IC; Department of Biological Sciences, Border Biomedical Research Center, University of Texas El Paso, El Paso, TX 79902, USA., Nosanchuk JD; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA., Nimrichter L; Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil.; Rede Micologia, RJ, FAPERJ, Rio de Janeiro 21941-902, RJ, Brazil.
المصدر: Journal of fungi (Basel, Switzerland) [J Fungi (Basel)] 2023 Oct 27; Vol. 9 (11). Date of Electronic Publication: 2023 Oct 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101671827 Publication Model: Electronic Cited Medium: Internet ISSN: 2309-608X (Electronic) Linking ISSN: 2309608X NLM ISO Abbreviation: J Fungi (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2015]-
مستخلص: Histoplasma capsulatum is the causative agent of histoplasmosis. Treating this fungal infection conventionally has significant limitations, prompting the search for alternative therapies. In this context, fungal extracellular vesicles (EVs) hold relevant potential as both therapeutic agents and targets for the treatment of fungal infections. To explore this further, we conducted a study using pharmacological inhibitors of chitinase (methylxanthines) to investigate their potential to reduce EV release and its subsequent impact on fungal virulence in an in vivo invertebrate model. Our findings revealed that a subinhibitory concentration of the methylxanthine, caffeine, effectively reduces EV release, leading to a modulation of H. capsulatum virulence. To the best of our knowledge, this is the first reported instance of a pharmacological inhibitor that reduces fungal EV release without any observed fungicidal effects.
References: Cell Microbiol. 2008 Aug;10(8):1695-710. (PMID: 18419773)
Mol Biol Evol. 2021 Apr 13;38(4):1339-1355. (PMID: 33185664)
Adv Exp Med Biol. 2019;1142:153-167. (PMID: 31102246)
J Extracell Vesicles. 2021 Aug;10(10):e12129. (PMID: 34377375)
Fungal Genet Biol. 2005 Nov;42(11):935-47. (PMID: 16214381)
Virulence. 2022 Dec;13(1):1900-1919. (PMID: 36266777)
J Biol Chem. 1991 Oct 15;266(29):19758-67. (PMID: 1918080)
Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2544-8. (PMID: 7708682)
J Clin Invest. 2021 Mar 1;131(5):. (PMID: 33373326)
Eukaryot Cell. 2007 Jan;6(1):48-59. (PMID: 17114598)
Trends Microbiol. 2001 Nov;9(11):541-6. (PMID: 11825714)
Front Immunol. 2021 Mar 16;12:654574. (PMID: 33796117)
Front Microbiol. 2018 Oct 02;9:2286. (PMID: 30333803)
Infect Dis Clin North Am. 2016 Mar;30(1):207-27. (PMID: 26897068)
Med Mycol. 2018 Oct 1;56(7):834-837. (PMID: 29253204)
J Biol Chem. 2020 Apr 24;295(17):5785-5794. (PMID: 32169899)
J Extracell Vesicles. 2019 Dec 19;9(1):1703244. (PMID: 32002167)
mSphere. 2019 Mar 20;4(2):. (PMID: 30894430)
Sci Rep. 2017 Sep 22;7(1):12187. (PMID: 28939893)
Microorganisms. 2020 Nov 18;8(11):. (PMID: 33217920)
Chem Biol. 2005 Sep;12(9):973-80. (PMID: 16183021)
J Infect Dis. 1997 Apr;175(4):905-14. (PMID: 9086148)
Mol Microbiol. 2004 Jul;53(1):153-65. (PMID: 15225311)
J Proteomics. 2014 Jan 31;97:177-86. (PMID: 23583696)
Mol Microbiol. 2006 Sep;61(5):1147-66. (PMID: 16925551)
Mycopathologia. 2012 Mar;173(2-3):83-91. (PMID: 21968902)
Front Cell Infect Microbiol. 2021 Jan 08;10:565571. (PMID: 33585262)
Microbes Infect. 2018 Oct - Nov;20(9-10):501-504. (PMID: 29471026)
mSphere. 2019 Apr 24;4(2):. (PMID: 31019001)
Cell Microbiol. 2020 Oct;22(10):e13238. (PMID: 32558196)
mBio. 2022 Feb 15;13(1):e0371821. (PMID: 35164559)
mSphere. 2019 Mar 27;4(2):. (PMID: 30918062)
Nat Methods. 2013 Aug;10(8):730-6. (PMID: 23921808)
Science. 2004 Jun 11;304(5677):1678-82. (PMID: 15192232)
Eukaryot Cell. 2008 Jan;7(1):58-67. (PMID: 18039940)
mBio. 2019 Apr 2;10(2):. (PMID: 30940711)
Antimicrob Agents Chemother. 2001 Mar;45(3):927-31. (PMID: 11181382)
Gene. 2023 May 15;864:147290. (PMID: 36804358)
Ecotoxicol Environ Saf. 2022 Aug;241:113800. (PMID: 35751931)
Cell Microbiol. 2015 Mar;17(3):389-407. (PMID: 25287304)
J Immunol Methods. 2012 Aug 31;382(1-2):224-8. (PMID: 22698786)
Virulence. 2013 Oct 1;4(7):597-603. (PMID: 23921374)
mSphere. 2016 Mar 30;1(2):. (PMID: 27303729)
Int J Nanomedicine. 2016 Aug 08;11:3715-30. (PMID: 27540288)
PLoS One. 2012;7(11):e48570. (PMID: 23144903)
Trends Microbiol. 2014 Mar;22(3):120-7. (PMID: 24530175)
mBio. 2022 Jun 28;13(3):e0030122. (PMID: 35420476)
mBio. 2021 Feb 9;12(1):. (PMID: 33563828)
Front Microbiol. 2016 Jul 08;7:1034. (PMID: 27458437)
PLoS Negl Trop Dis. 2014 Aug 21;8(8):e3100. (PMID: 25144374)
Sci Rep. 2018 May 23;8(1):8065. (PMID: 29795301)
Curr Genet. 2016 May;62(2):243-54. (PMID: 26527115)
Infect Dis Clin North Am. 2016 Mar;30(1):51-83. (PMID: 26739608)
Mol Immunol. 2021 Jul;135:137-146. (PMID: 33895578)
Curr Opin Microbiol. 2013 Aug;16(4):414-20. (PMID: 23628115)
J Fungi (Basel). 2018 Nov 27;4(4):. (PMID: 30486393)
معلومات مُعتمدة: U54 MD007592 United States MD NIMHD NIH HHS; AI165204 United States NH NIH HHS; R21 AI124797 United States NH NIH HHS
فهرسة مساهمة: Keywords: Histoplasma capsulatum; caffeine; chitinase; exosomes; extracellular vesicles; histoplasmosis; methylxanthine
تواريخ الأحداث: Date Created: 20231124 Latest Revision: 20240210
رمز التحديث: 20240210
مُعرف محوري في PubMed: PMC10672645
DOI: 10.3390/jof9111052
PMID: 37998859
قاعدة البيانات: MEDLINE