دورية أكاديمية
أعرض في EDS

Human Pancreatic Islets React to Glucolipotoxicity by Secreting Pyruvate and Citrate.

التفاصيل البيبلوغرافية
العنوان: Human Pancreatic Islets React to Glucolipotoxicity by Secreting Pyruvate and Citrate.
المؤلفون: Perrier J; Laboratoire CarMeN, UMR INSERM U1060/INRAE U1397, University of Lyon, Université Claude Bernard Lyon 1, 69310 Pierre-Bénite, France., Nawrot M; Laboratoire CarMeN, UMR INSERM U1060/INRAE U1397, University of Lyon, Université Claude Bernard Lyon 1, 69310 Pierre-Bénite, France., Madec AM; Laboratoire CarMeN, UMR INSERM U1060/INRAE U1397, University of Lyon, Université Claude Bernard Lyon 1, 69310 Pierre-Bénite, France., Chikh K; Laboratoire CarMeN, UMR INSERM U1060/INRAE U1397, University of Lyon, Université Claude Bernard Lyon 1, 69310 Pierre-Bénite, France.; Department of Endocrinology and Diabetes, Hospices Civils de Lyon, Hopital Lyon Sud, 69310 Pierre-Bénite, France., Chauvin MA; Laboratoire CarMeN, UMR INSERM U1060/INRAE U1397, University of Lyon, Université Claude Bernard Lyon 1, 69310 Pierre-Bénite, France., Damblon C; Unité de Recherche MolSys, Faculté des Sciences, Université de Liège, 99131 Liège, Belgium., Sabatier J; Laboratory of Cell Therapy for Diabetes (LTCD), PRIMS Facility, Institute for Regenerative Medicine and Biotherapy (IRMB), University Hospital of Montpellier, 34295 Montpellier, France., Thivolet CH; Laboratoire CarMeN, UMR INSERM U1060/INRAE U1397, University of Lyon, Université Claude Bernard Lyon 1, 69310 Pierre-Bénite, France.; Department of Endocrinology and Diabetes, Hospices Civils de Lyon, Hopital Lyon Sud, 69310 Pierre-Bénite, France., Rieusset J; Laboratoire CarMeN, UMR INSERM U1060/INRAE U1397, University of Lyon, Université Claude Bernard Lyon 1, 69310 Pierre-Bénite, France., Rautureau GJP; Centre de Résonance Magnétique Nucléaire à Très Hauts Champs, UMR 5082 CNRS, ENS Lyon, UCBL, Université de Lyon, 69100 Villeurbanne, France., Panthu B; Laboratoire CarMeN, UMR INSERM U1060/INRAE U1397, University of Lyon, Université Claude Bernard Lyon 1, 69310 Pierre-Bénite, France.
المصدر: Nutrients [Nutrients] 2023 Nov 15; Vol. 15 (22). Date of Electronic Publication: 2023 Nov 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI Publishing
مواضيع طبية MeSH: Diabetes Mellitus, Type 2*/metabolism , Islets of Langerhans* , Insulin-Secreting Cells*/metabolism, Rats ; Animals ; Humans ; Pyruvic Acid/metabolism ; Citric Acid/pharmacology ; Citric Acid/metabolism ; Glucose/pharmacology ; Glucose/metabolism ; Insulin/metabolism
مستخلص: Progressive decline in pancreatic beta-cell function is central to the pathogenesis of type 2 diabetes (T2D). Here, we explore the relationship between the beta cell and its nutritional environment, asking how an excess of energy substrate leads to altered energy production and subsequent insulin secretion. Alterations in intracellular metabolic homeostasis are key markers of islets with T2D, but changes in cellular metabolite exchanges with their environment remain unknown. We answered this question using nuclear magnetic resonance-based quantitative metabolomics and evaluated the consumption or secretion of 31 extracellular metabolites from healthy and T2D human islets. Islets were also cultured under high levels of glucose and/or palmitate to induce gluco-, lipo-, and glucolipotoxicity. Biochemical analyses revealed drastic alterations in the pyruvate and citrate pathways, which appear to be associated with mitochondrial oxoglutarate dehydrogenase (OGDH) downregulation. We repeated these manipulations on the rat insulinoma-derived beta-pancreatic cell line (INS-1E). Our results highlight an OGDH downregulation with a clear effect on the pyruvate and citrate pathways. However, citrate is directed to lipogenesis in the INS-1E cells instead of being secreted as in human islets. Our results demonstrate the ability of metabolomic approaches performed on culture media to easily discriminate T2D from healthy and functional islets.
References: Cell Metab. 2013 Aug 6;18(2):162-85. (PMID: 23791483)
Curr Diab Rep. 2014 Jun;14(6):492. (PMID: 24740729)
Metabolomics. 2019 Mar 29;15(4):55. (PMID: 30927092)
Diabetes Res Clin Pract. 2011 Aug;93 Suppl 1:S37-46. (PMID: 21864750)
Diabetes. 2019 Sep;68(9):1778-1794. (PMID: 31175102)
Cell Metab. 2019 Jun 4;29(6):1422-1432.e3. (PMID: 30879985)
Int J Mol Sci. 2020 Sep 18;21(18):. (PMID: 32961865)
Mol Cell Endocrinol. 2012 Nov 25;364(1-2):1-27. (PMID: 22885162)
J Biol Chem. 2017 May 5;292(18):7407-7422. (PMID: 28280244)
Nat Cell Biol. 2003 Apr;5(4):330-5. (PMID: 12640462)
Nucleic Acids Res. 2018 Jan 4;46(D1):D608-D617. (PMID: 29140435)
Diabetologia. 2009 Mar;52(3):463-76. (PMID: 19165461)
Diabetes. 2020 Mar;69(3):273-278. (PMID: 31519699)
Stem Cells Int. 2019 May 14;2019:9323864. (PMID: 31223312)
Adv Exp Med Biol. 1997;426:259-66. (PMID: 9544283)
Metabolism. 2021 Nov;124:154870. (PMID: 34480921)
J Biol Chem. 2002 Apr 5;277(14):11746-55. (PMID: 11821387)
Endocr Rev. 2008 May;29(3):351-66. (PMID: 18048763)
Cell Metab. 2017 Jun 6;25(6):1334-1347.e4. (PMID: 28591636)
Nat Protoc. 2007;2(11):2692-703. (PMID: 18007604)
Nat Commun. 2019 Jun 6;10(1):2474. (PMID: 31171772)
Anal Bioanal Chem. 2020 Sep;412(22):5453-5463. (PMID: 32556564)
J Biol Chem. 1994 Feb 18;269(7):4895-902. (PMID: 8106462)
Anal Chim Acta. 2016 Aug 31;934:194-202. (PMID: 27506360)
Nat Rev Endocrinol. 2017 May;13(5):268-277. (PMID: 27834384)
J Biol Chem. 2011 May 27;286(21):18383-96. (PMID: 21454710)
J Clin Invest. 1992 Oct;90(4):1263-8. (PMID: 1401063)
Biochim Biophys Acta. 2013 Mar;1830(3):2583-90. (PMID: 23153904)
Sci Rep. 2021 Apr 22;11(1):8712. (PMID: 33888788)
Diabetes. 2017 Aug;66(8):2188-2200. (PMID: 28559245)
Sci Rep. 2020 Sep 22;10(1):15413. (PMID: 32963286)
J Biol Chem. 1997 Jan 31;272(5):3091-8. (PMID: 9006960)
J Mol Biol. 2020 Mar 6;432(5):1429-1445. (PMID: 31325441)
J Biol Chem. 2009 Nov 20;284(47):32395-404. (PMID: 19797055)
PLoS One. 2014 Jun 18;9(6):e100017. (PMID: 24940599)
J Diabetes. 2014 Mar;6(2):113-9. (PMID: 24134160)
Int Rev Cell Mol Biol. 2021;363:169-202. (PMID: 34392929)
فهرسة مساهمة: Keywords: GSIS; INS-1E; NMR; glucolipotoxicity; human pancreatic islets; insulin content; quantitative metabolomics; type 2 diabetes
المشرفين على المادة: 8558G7RUTR (Pyruvic Acid)
2968PHW8QP (Citric Acid)
IY9XDZ35W2 (Glucose)
0 (Insulin)
تواريخ الأحداث: Date Created: 20231125 Date Completed: 20231127 Latest Revision: 20231127
رمز التحديث: 20231127
مُعرف محوري في PubMed: PMC10674605
DOI: 10.3390/nu15224791
PMID: 38004183
قاعدة البيانات: MEDLINE
الوصف
تدمد:2072-6643
DOI:10.3390/nu15224791