دورية أكاديمية
Resazurin microplate test method for rapid determination of colistin resistance in carbapenem-resistant Acinetobacter baumanii isolates.
| العنوان: | Resazurin microplate test method for rapid determination of colistin resistance in carbapenem-resistant Acinetobacter baumanii isolates. |
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| المؤلفون: | Yildirim K; Department of Nutrition and Dietetics, Faculty of Health Sciences, Akdeniz University, Antalya, Turkey.; Tuberculosis Research Center, Akdeniz University, Antalya, Turkey., Simsek E; Department of Nutrition and Dietetics, Faculty of Health Sciences, Akdeniz University, Antalya, Turkey.; Tuberculosis Research Center, Akdeniz University, Antalya, Turkey.; Department of Medical Biotechnology, Institute of Health Sciences, Akdeniz University, Antalya, Turkey., Kocak O; Tuberculosis Research Center, Akdeniz University, Antalya, Turkey., Bozkurt S; Tuberculosis Research Center, Akdeniz University, Antalya, Turkey., Koyuncu Ozyurt O; Department of Medical Microbiology, Faculty of Medicine, Akdeniz University, Antalya, Turkey., Coban AY; Department of Nutrition and Dietetics, Faculty of Health Sciences, Akdeniz University, Antalya, Turkey.; Tuberculosis Research Center, Akdeniz University, Antalya, Turkey.; Department of Medical Biotechnology, Institute of Health Sciences, Akdeniz University, Antalya, Turkey. |
| المصدر: | Laboratory medicine [Lab Med] 2024 May 02; Vol. 55 (3), pp. 380-385. |
| نوع المنشور: | Journal Article; Evaluation Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0250641 Publication Model: Print Cited Medium: Internet ISSN: 1943-7730 (Electronic) Linking ISSN: 00075027 NLM ISO Abbreviation: Lab Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oct. 2015- : Oxford, England: Oxford University Press Original Publication: Philadelphia, Lippincott. |
| مواضيع طبية MeSH: | Colistin*/pharmacology , Acinetobacter baumannii*/drug effects , Acinetobacter baumannii*/isolation & purification , Xanthenes*/pharmacology , Microbial Sensitivity Tests*/methods , Oxazines*/pharmacology , Anti-Bacterial Agents*/pharmacology , Carbapenems*/pharmacology, Humans ; Drug Resistance, Bacterial/drug effects ; Acinetobacter Infections/microbiology ; Acinetobacter Infections/drug therapy |
| مستخلص: | Background: Colistin-resistant Acinetobacter baumannii isolates are extremely important pathogens for hospital-acquired infections. Objective: To investigate the effectiveness of the resazurin microplate assay (REMA) for the rapid determination of colistin resistance. Methods: Susceptibility for colistin was investigated in vitro by the broth microdilution method (BMD) and the resazurin microplate assay (REMA) on 106 carbapenem-resistant Acinetobacter baumannii isolates. Results: The results of both test methods were compared, and the categorical agreement between them was found to be 100%. No minor, major, or very major discrepancy was observed between the 2 methods. Conclusions: The most important advantages of REMA are that the results are obtained within 6 hours compared to the reference method, that it is easy to evaluate because it is colorimetric, and that the susceptibility result can be reported to the clinician on the same day as bacterial identification. (© The Author(s) 2023. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.) |
| فهرسة مساهمة: | Keywords: Acinetobacter baumannii; carbapenem resistance; colistin; colorimetric test; rapid antibiogram susceptibility test; resazurin |
| المشرفين على المادة: | Z67X93HJG1 (Colistin) 1FN9YD6968 (resazurin) 0 (Xanthenes) 0 (Oxazines) 0 (Anti-Bacterial Agents) 0 (Carbapenems) |
| تواريخ الأحداث: | Date Created: 20231125 Date Completed: 20240502 Latest Revision: 20240502 |
| رمز التحديث: | 20240502 |
| DOI: | 10.1093/labmed/lmad099 |
| PMID: | 38007395 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1943-7730 |
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| DOI: | 10.1093/labmed/lmad099 |