دورية أكاديمية
Cloning, expression and purification of Eimeria maxima gametocyte antigen-EmGam56 for control of poultry coccidiosis.
| العنوان: | Cloning, expression and purification of Eimeria maxima gametocyte antigen-EmGam56 for control of poultry coccidiosis. |
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| المؤلفون: | Ramalingam V; Translational Research Platform for Veterinary Biologicals (TRPVB), Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, 600051 Chennai, India., Muthusamy R; Translational Research Platform for Veterinary Biologicals (TRPVB), Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, 600051 Chennai, India.; Department of Microbiology, Centre for Infectious Diseases, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, 600077 India., Bohra K; Translational Research Platform for Veterinary Biologicals (TRPVB), Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, 600051 Chennai, India., Palavesam A; Translational Research Platform for Veterinary Biologicals (TRPVB), Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, 600051 Chennai, India., Gopal D; Translational Research Platform for Veterinary Biologicals (TRPVB), Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, 600051 Chennai, India. |
| المصدر: | Journal of parasitic diseases : official organ of the Indian Society for Parasitology [J Parasit Dis] 2023 Dec; Vol. 47 (4), pp. 773-777. Date of Electronic Publication: 2023 Aug 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer India Country of Publication: India NLM ID: 9713059 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0971-7196 (Print) Linking ISSN: 09717196 NLM ISO Abbreviation: J Parasit Dis Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: <2009-> : New Dehli : Springer India Original Publication: Lucknow, India : Indian Society for Parasitology, c1995- |
| مستخلص: | Poultry coccidiosis is an important devitalizing enteric protozoan disease caused by a group of obligatory intracellular apicomplexan parasites of the Genus Eimeria contributing to major economic loss in commercial poultry worldwide. As the current method of chemotherapeutic control using ionophores in feed had led to development of drug resistant isolates, the need for development of prophylactic vaccines is the most viable alternate and eco-friendly control strategy as on date. Of the several candidate vaccines, the Em Gam 56 is one of the most promising candidates which protect the birds against E. maxima , E. tenella and E. acervulina , the three most pathogenic coccidian species infecting commercial chicken. Em Gam56 is a major wall forming component of macrogametocyte of E. maxima and a candidate with high immunogenicity and low virulence. The present study was planned and carried out for the generation of E.coli expressed recombinant gametocyte antigen- Em Gam56 using pET 28(a+) as cloning vector and BL21 DE3 (pLysS) as prokaryotic expression system in a Bio-fermentor (New Brunswick™ Scientific BioFlo 310). The recombinant protein was purified by conventional (Ammonium sulphate precipitation) and by automatic purification system (AKTA prime) in Ni-NTA column for a planned immunization trial with experimental chickens. Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest. (© Indian Society for Parasitology 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.) |
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| فهرسة مساهمة: | Keywords: Eimeria maxima; EmGam56; Gametocyte antigen; Purification; Recombinant protein |
| تواريخ الأحداث: | Date Created: 20231127 Latest Revision: 20231128 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC10667185 |
| DOI: | 10.1007/s12639-023-01610-w |
| PMID: | 38009159 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 0971-7196 |
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| DOI: | 10.1007/s12639-023-01610-w |